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actc1b-mKate2-ARF1

Plasmid

#109535

Purpose

Muscle specific zebrafish expression of mKate2 fused to ARF1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ARF1 (arf1 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAPL1 no-stop

Plasmid

#123210

Purpose

Gateway entry clone encoding human GABARAPL1 lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Use

Gateway entry vector / entry clone

Mutation

Stop codon removed

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 R159K

Plasmid

#123626

Purpose

Expresses GgKDELR2 with Arg159Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Arg159Lys

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 H12A

Plasmid

#123619

Purpose

Expresses GgKDELR2 with His12Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Tags

TEV-GFP-His

Expression

Yeast

Mutation

His12Ala

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 R47A

Plasmid

#123620

Purpose

Expresses GgKDELR2 with Arg47Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Arg47Ala

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 R47K

Plasmid

#123621

Purpose

Expresses GgKDELR2 with Arg47Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Arg47Lys

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 E127A

Plasmid

#123622

Purpose

Expresses GgKDELR2 with Glu127Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Glu127Ala

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 E127Q

Plasmid

#123623

Purpose

Expresses GgKDELR2 with Glu127Gln mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Glu127Gln

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 Y158F

Plasmid

#123624

Purpose

Expresses GgKDELR2 with Tyr158Phe mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Tyr158Phe

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 R159A

Plasmid

#123625

Purpose

Expresses GgKDELR2 with Arg159Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Arg159Ala

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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gh105

Plasmid

#106775

Purpose

expression of gRNA targeting B3GALT1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B3GALT1 (B3GALT1 Human)

Expression

Mammalian

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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PD-L1-miSFIT-9-G, 15-C

Plasmid

#124688

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-L1 (CD274 Human)

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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gh2

Plasmid

#106686

Purpose

expression of gRNA targeting GALNT2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT2 (GALNT2 Human)

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR148

Plasmid

#106048

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR148; Opto-GPR148; E2)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR148 (GPR148 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.26 EC10-Fc-His

Plasmid

#72189

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.26

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.23 EC10-Fc-His

Plasmid

#72187

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.23 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.23

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.26 EC10-AP-His

Plasmid

#72063

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.26

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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gh263

Plasmid

#106862

Purpose

expression of gRNA targeting ALG10

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ALG10 (ALG10 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mCherry-CHMP2B-siRNAres

Plasmid

#115333

Purpose

expresses siRNA-resistant mCherry-CHMP2B in mammalian cells

Depositor

Sanford Simon

Article

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

CHMP2B (CHMP2B Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

mutated to be resistant to siRNA knockdown

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DLG1-1/1

Plasmid

#91448

Purpose

Protein expression and purification of human SH3 domain construct DLG1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DLG1-1/1 (DLG1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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