We narrowed to 4,247 results for: Pet2

1. pJK040 (pET-PurQ-A128T)

   Plasmid

   #73840

   Purpose

   Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)

   Depositor

   JoAnne Stubbe, T. J. Kappock

   Article

   Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

   Insert

   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A128T mutant

   Promoter

   T7

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJK044 (pET-PurQ-wt)

   Plasmid

   #73839

   Purpose

   Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)

   Depositor

   JoAnne Stubbe, T. J. Kappock

   Article

   Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

   Insert

   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJK598

   Plasmid

   #72523

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with C-terminal His6 tag, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurL)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   synthetic gene

   Promoter

   T7 and n/a

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJK560

   Plasmid

   #71585

   Purpose

   Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with Arg232>His mutant (YfdE-R232H)

   Depositor

   T. J. Kappock

   Article

   Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

   Insert

   acetyl-CoA:oxalate CoA-transferase (yfdE )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed arginine-232 to histidine

   Promoter

   T7 promoter

   Available since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJK504

   Plasmid

   #71584

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC) with C-terminal His6 tag and Cys357>Tyr mutant (AarCH6-C357Y)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Acetic Acid Bacteria. 2(s1):e3 (2013)

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes cysteine-357 to tyrosine

   Promoter

   T7

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJK612

   Plasmid

   #72533

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurL)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   residues 1-69 only; C-terminal domain removed and…

   Promoter

   T7 and n/a

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJK613

   Plasmid

   #72534

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with His6 tag; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurLH6)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   residues 1-69 only; C-terminal domain removed and…

   Promoter

   T7 and n/a

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJK619

   Plasmid

   #72536

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His-tag; hypothet. protein; glutaminase subunit; large subunit (H6AaPurS, AaOrfY, AaPurQ, AaPurL)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   synthetic gene

   Promoter

   T7 and n/a

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pfMDH_K381A

   Plasmid

   #67732

   Purpose

   Expresses K381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Article

   Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

   Insert

   Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
   

   Use

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   Changed lysine 381 to alanine

   Promoter

   T7

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pfMDH_N300D

    Plasmid

    #67728

    Purpose

    Expresses N300D mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed asparagine 300 to aspartate

    Promoter

    T7

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pfMDH_R373A

    Plasmid

    #67731

    Purpose

    Expresses R373A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed arginine 373 to alanine

    Promoter

    T7

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pfMDH_N300S

    Plasmid

    #67729

    Purpose

    Expresses N300S mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed asparagine 300 to serine

    Promoter

    T7

    Available since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

13. 4QL1

    Plasmid

    #61942

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    WDR5:APC041-G05:C36415 (WDR5 Human)
    

    Use

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. GMIP

    Plasmid

    #32046

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    GMIP (GMIP Human)
    

    Use

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    amino acids 80-357 only

    Promoter

    Available since

    Jan. 13, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

15. sankE3_5

    Plasmid

    #24893

    Depositor

    David Baker

    Article

    Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

    Insert

    sank E3_5
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pSKB2-TRexR90D

    Plasmid

    #31350

    Depositor

    Clara Kielkopf

    Article

    McLaughlin et al Mol Cell. 2010 May 28. 38(4):563-75.

    Insert

    Thermus aquaticus Redox Sensing Repressor (R90D)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Arginine 90 to Aspartic Acid

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

17. PrbC5

    Plasmid

    #24787

    Depositor

    David Baker

    Article

    Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

    Insert

    Prb
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Asp11Gly, Asp83Asn, Val92Ala, Glu119Gly, and Glu1…

    Promoter

    Available since

    July 11, 2011

    Availability

    Academic Institutions and Nonprofits only

18. phtsResA

    Plasmid

    #25914

    Depositor

    Johann Deisenhofer

    Article

    Colbert et al Proc Natl Acad Sci U S A. 2006 Mar 21. 1

    Insert

    His-tagged soluble ResA (resA Bacillus subtilis)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Start at Serine 37 to remove n-terminal transmemb…

    Promoter

    Available since

    Dec. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

19. ARL5:H14-K177 (2H16)

    Plasmid

    #25526

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ARL5:H14-K177 (ARL5A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids H14-K177

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

20. GNE

    Plasmid

    #25255

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    GNE:T406-R720 (GNE Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids T406-R720

    Promoter

    Available since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits