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pJOE5751.1

Plasmid

#135083

Purpose

pBR322-based L-rhamnose-inducible vector.

Depositor

Josef Altenbuchner

Article

Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

Insert

P-rhaP-BAD-His tag-eGFP

Expression

Bacterial

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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MTK3_020

Plasmid

#123735

Purpose

Encodes rapamycin and tamoxifen activated split-dCAS9 (C-terminal) as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

ERT2-fkbp-spdCa9(205-1368)

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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JN607: pMVP/SB-DEST

Plasmid

#121860

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IZ401: pMVP/Lenti/Neo-DEST

Plasmid

#121850

Purpose

pMVP destination vector, empty Lentivirus vector backbone w/ Neo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Lentiviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-SFXN2

Plasmid

#110636

Purpose

Retroviral expression of Flag-SFXN2 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

SFXN2 (SFXN2 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pPU-Empty-miR30-TRIM5

Plasmid

#132936

Purpose

All-in-one control rescue-TRIM5 shRNA knockdown vector

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

control shRNA

Use

Lentiviral and RNAi

Tags

HA

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-SFXN4

Plasmid

#110638

Purpose

Retroviral expression of Flag-SFXN4 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

SFXN4 (SFXN4 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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KTK_136

Plasmid

#180520

Purpose

Empty backbone for D1.1 cloning, contains sfGFP dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSK260

Plasmid

#129252

Purpose

dual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporter

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Sup35 NM yTRAP sensor reporting on mKate2

Expression

Yeast

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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HOXC8-pSG5

Plasmid

#21001

Depositor

Corey Largman

Article

Lawrence et al Cell Growth Differ. 1993 Aug . 4(8):665-

Insert

HOXC8 (HOXC8 Human)

Expression

Mammalian

Mutation

missing first 24 N-terminal amino acids of coding…

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pITGFP89

Plasmid

#83560

Purpose

Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF1 promoter -yEGFP-CLN2PEST

Expression

Yeast

Promoter

TEF1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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KK402: pMVP/Zeo-DEST

Plasmid

#121859

Purpose

pMVP destination vector, empty expression vector backbone w/ Zeo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pBad-CA TAG93

Plasmid

#105667

Purpose

expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 93 and C-terminal 6x his tag

Depositor

Ryan Mehl

Article

Mehl et al 2020

Insert

human carbonic anhydrase II thermostable variant (CA2 Human)

Tags

6x his tag

Expression

Bacterial

Mutation

TAG stop codon at site 93

Promoter

pBad/arabinose

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pKT3Ts-goldyTALEN

Plasmid

#80330

Purpose

Updated TALEN vector; compatible with the Golden Gate TALEN assembly protocol. More stable backbone with reduced recombination compared to pT3Ts-goldyTALEN due to the removal of a lacZ sequence

Depositor

Jeffrey Essner

Article

Welker et al Hum Gene Ther. 2016 Jun;27(6):423-4. doi

Type

Empty backbone

Use

TALEN

Expression

Bacterial

Promoter

T3

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pUHG10.3-TetO-Rs1

Plasmid

#16313

Depositor

Bruce Conklin

Article

Hsiao et al Proc Natl Acad Sci U S A. 2008 Jan 29. 1

Insert

h5HT4b Serotonin Receptor (Rs1 D100A RASSL) (RS1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Added FLAG tag; Changed Aspartic Acid 100 to Alan…

Available Since

Jan. 3, 2008

Availability

Academic Institutions and Nonprofits only

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GST-FOXO3a 1-506aa

Plasmid

#10826

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

GST-FOXO3a 1-506aa (FOXO3 Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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GST-FOXO3a TM 1-506aa

Plasmid

#8351

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FOXO3a 1-506aa (FOXO3 Human)

Tags

GST

Expression

Bacterial

Mutation

T32A, S253A, S315A

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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MTK3_001

Plasmid

#123952

Purpose

Encodes nuclear localized PHR domain of CRY2 fused to activating domain of p65 as Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

3XNLS::CRY2PHR::p65

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSK226

Plasmid

#129268

Purpose

dual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporter

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Rnq1

Expression

Yeast

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pONSY

Plasmid

#111873

Purpose

"Capsaspora owczarzaki" expression vector (backbone) modified from the pCR2.1. It bears the promoter and terminator regions from the endogenous Elongation Factor 1 alpha (EF1a) gene (CAOG_07807).

Depositor

Inaki Ruiz-Trillo

Article

Parra-Acero et al Development. 2018 May 11. pii: dev.16210

Type

Empty backbone

Use

Capsaspora owczarzaki

Promoter

Elongation Factor 1 alpha (EF1a) from Capsaspora

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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