167,601 results

1. pGP-AAV-syn-jGCaMP8f-WPRE (AAV Retrograde)

   Viral Prep

   #162376-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-jGCaMP8f-WPRE (#162376). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8f-WPRE plasmid DNA. Syn-driven expression of ultrafast calcium sensor GCaMP8f. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   Syn

   Available Since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

2. YE1-BE4max

   Plasmid

   #138155

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   rAPOBEC1(YE1)-nSpCas9-UGI-UGI
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Within rAPOBEC1 W90Y + R126E; within Cas9 D10A

   Promoter

   CMV

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. EB3-mScarlet-I

   Plasmid

   #98826

   Purpose

   In vivo visualization of microtuble plus ends (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   EB3
   

   Tags

   mScarlet-I

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFRT_TO_DESTFLAGHA

   Plasmid

   #26361

   Depositor

   Thomas Tuschl

   Article

   Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

   Type

   Empty backbone

   Tags

   FLAG/HA

   Expression

   Mammalian

   Available Since

   Sept. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pAM1579

   Plasmid

   #40240

   Depositor

   Susan Golden

   Article

   Andersson et al Methods Enzymol. 2000;305:527-42.

   Insert

   Km Resistance, neutral site II integration platform for Synechococcus
   

   Use

   Cyanobacteria cloning vector

   Promoter

   n/a

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. dCas9-ddMSK1

   Plasmid

   #165603

   Purpose

   Expresses Sp dCas9 fused to truncated inactive human MSK1 (42-802, D195A, D565A)

   Depositor

   Isaac Hilton

   Article

   Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

   Insert

   S. Pyogenes dCas9 with c-terminal truncated inactive human Mitogen- and stress-activated protein kinase-1 (42-802, D195A, D565A) (RPS6KA5 S. Pyogenes, Synthetic, Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTW2082

   Plasmid

   #236576

   Purpose

   ISYmu1 PDS3 gRNA12 TRV2 Architecture_B

   Depositor

   Steven Jacobsen

   Article

   Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

   Insert

   ISYmu1 TnpB
   

   Expression

   Plant

   Available Since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHCMV_DENV3_PrME

   Plasmid

   #207265

   Purpose

   Mammalian expression of Dengue virus 3 PrME protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   DENV3_PrME (POLY Dengue virus 3)
   

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Str-KDEL_SBP-EGFP-LAMP2

   Plasmid

   #222321

   Purpose

   Synchronize the trafficking of LAMP2 from the ER.

   Depositor

   Franck Perez

   Article

   Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

   Insert

   Streptavidin-KDEL and LAMP2 fused to SBP-EGFP (LAMP2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFCIV-CEACAM6

    Plasmid

    #149303

    Purpose

    lentiviral packaging vector for CEACAM6 expression.

    Depositor

    James Fleckenstein

    Article

    Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

    Insert

    CEACAM6 (CEACAM6 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    ubiquitin C

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmVen-DHX9

    Plasmid

    #211409

    Purpose

    mammalian expression of human DHX9 N-terminally fused to mVenus

    Depositor

    Helmut Pospiech

    Insert

    DHX9 (DHX9 Human)
    

    Tags

    mVenus

    Expression

    Mammalian

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAUC40

    Plasmid

    #71298

    Purpose

    Suicide Vector, Gateway attR-Chloramphenicol resistance cassette cloned in pKNG101

    Depositor

    Susanne von Bodman

    Article

    Carlier et al Mol Microbiol. 2009 Nov;74(4):903-13. do

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAVS1-NDi-CRISPRi (Gen2)

    Plasmid

    #73498

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCJ1021

    Plasmid

    #228761

    Purpose

    Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.

    Depositor

    Bradley Yoder

    Insert

    Ift88 gRNAs (Ift88 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. K785R Smarca4-sfGFP

    Plasmid

    #107057

    Purpose

    Expression of dominant-negative K785R Smarca4 (Brg1) with C-terminal super-folder GFP fusion.

    Depositor

    Jerry Crabtree, Courtney Hodges

    Article

    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    

    Use

    Lentiviral

    Tags

    Super-folder GFP (sfGFP)

    Expression

    Mammalian

    Mutation

    K785R

    Promoter

    CAG

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICSL11024 (pICH47732::NOSp-NPTII-OCST)

    Plasmid

    #51144

    Purpose

    Confers KAN resistance in planta

    Depositor

    Jonathan D Jones

    Insert

    NOSp-NPTII-OCST
    

    Available Since

    Feb. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. Str-KDEL_CD9-SBP-mCherry

    Plasmid

    #222329

    Purpose

    Synchronize the trafficking of CD9 from the ER.

    Depositor

    Franck Perez

    Article

    Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

    Insert

    Streptavidin-KDEL and CD9 fused to SBP-mCherry (CD9 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pR-mAmetrine

    Plasmid

    #228598

    Purpose

    Mammalian expression of mAmetrine, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mAmetrine
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. dCAS9-VP64_GFP (Concentrated Lentiviral Prep)

    Viral Prep

    #61422-LVC

    Purpose

    Ready-to-use Concentrated Lentiviral Prep particles produced from dCAS9-VP64_GFP (#61422). In addition to the viral particles, you will also receive purified dCAS9-VP64_GFP plasmid DNA.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Promoter

    EF1a

    Available Since

    Aug. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pET His6 Thioredoxin TEV co-transformation cloning vector (13K-T)

    Plasmid

    #48322

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6 and Thioredoxin

    Expression

    Bacterial

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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