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Addgene

We narrowed to 11,256 results for: NSI

Showing: 7201 - 7240 of 11256 results
  1. pLCKO2

    Plasmid
    #125518
    Purpose
    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. pC1-oROS-HT-CaaX

    Plasmid
    #216417
    Purpose
    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the intracellular site of cell membranes.
    Depositor
    Insert
    oROS-HT-CaaX
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pNOC_hfnCas12a_Nlux_(-HH)crRNA NR1

    Plasmid
    #176250
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1 without the hammerhead ribozym
    Depositor
    Insert
    humanized fnCas12a
    Use
    CRISPR and Synthetic Biology; Expression in micro…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. FLAG-LoxP-GFP-LoxP-SNAP-TERT

    Plasmid
    #71391
    Purpose
    Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes GFP marker flanked by LoxP sites for selection.
    Depositor
    Insert
    TERT (TERT Human)
    Use
    Hr donor
    Tags
    FLAG-SNAP
    Expression
    Mutation
    Promoter
    Endogenous TERT promotor
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. pDisplay-oROS-HT

    Plasmid
    #216416
    Purpose
    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the extracellular site of cell membranes.
    Depositor
    Insert
    pDisplay-oROS-HT
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pSLIK CA ROCK2

    Plasmid
    #84649
    Purpose
    Lentiviral expression of doxycycline-inducible constitutively active ROCK2 and co-expression of Venus
    Depositor
    Insert
    ROCK2 (ROCK2 Bovine)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Constitutive Active (please see depositor comment…
    Promoter
    Available since
    Dec. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
  7. pQ54-VC

    Plasmid
    #175060
    Purpose
    expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ54) in mammalian cells
    Depositor
    Insert
    polyQ54 (HTT Human)
    Use
    Tags
    mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pQ25-VC

    Plasmid
    #175062
    Purpose
    expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ25) in mammalian cells
    Depositor
    Insert
    polyQ25 (HTT Human)
    Use
    Tags
    mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. pQ39-VC

    Plasmid
    #175061
    Purpose
    expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ39) in mammalian cells
    Depositor
    Insert
    polyQ39 (HTT Human)
    Use
    Tags
    mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. MCP-MSN

    Plasmid
    #210704
    Purpose
    This plasmid express EF1a promoter driven MCP-MSN transactivation module
    Depositor
    Insert
    MCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
  11. pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS

    Plasmid
    #154774
    Purpose
    plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma PylRS for ochre suppression in transient or stable, piggybac-mediated, integration
    Depositor
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    in PylT: UUA anticodon and G10A, G14U, G16U, C20U…
    Promoter
    EF1
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
  12. MCP-eN3x9

    Plasmid
    #210706
    Purpose
    This plasmid express EF1a promoter driven MCP-eN3x9 transactivation module
    Depositor
    Insert
    MCP fused to transactivation domain comprising eNRF2 and 3x 9aa Transactivation domain
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 7201 - 7240 of 11256 results