167,601 results

1. pLenti6.3-SlugCas9-BlastR

   Plasmid

   #220965

   Purpose

   Expresses human codon-optimized SlugCas9

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   SlugCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMA3790

   Plasmid

   #70110

   Purpose

   mtDNA elimination/production of rho-0 cells

   Depositor

   Mikhail Alexeyev

   Article

   Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

   Inserts

   EGFP
   hUNG1 Y147A
   Neo
   

   Tags

   None, human OTC mitochondrial targeting sequence,…

   Expression

   Mammalian

   Mutation

   Y147A, aa's 1-77 removed

   Promoter

   CMV, EF1a, and SV40 and native

   Available Since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH-EF1-FHC-POLQ

   Plasmid

   #64875

   Purpose

   lentiviral expression of human POLQ

   Depositor

   Richard Wood

   Article

   Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

   Insert

   POLQ (POLQ Human)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Aug. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-cT3G-cERP2-NR5A1

   Plasmid

   #192915

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of NR5A1

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   NR5A1 (NR5A1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTW2154

   Plasmid

   #236578

   Purpose

   ISYmu1 CHLl1 gRNA4 TRV2 Architecture_B

   Depositor

   Steven Jacobsen

   Article

   Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

   Insert

   ISYmu1 TnpB
   

   Expression

   Plant

   Available Since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-C1-ER alpha

   Plasmid

   #28230

   Purpose

   Mammalian expression of Estrogen Receptor alpha fused to EGFP

   Depositor

   Michael Mancini

   Article

   Stenoien et al Mol Endocrinol. 2000 Apr . 14(4):518-34.

   Insert

   Estrogen Receptor alpha (ESR1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   April 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-LC3

   Plasmid

   #21073

   Purpose

   Mammalian expression of rat LC3 fused to EGFP

   Depositor

   Tamotsu Yoshimori

   Article

   Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   First ATG is not included.

   Promoter

   CMV

   Available Since

   May 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40 (AAV1)

   Viral Prep

   #100850-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40 (#100850). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent NES-jRCaMP1b calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Promoter

   Syn

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pCAG G-CEPIA1er

   Plasmid

   #105012

   Purpose

   ER calcium sensor

   Depositor

   Franck Polleux

   Article

   Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

   Insert

   G-CEPIA1er
   

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLminP_Luc2P_RE27

    Plasmid

    #90369

    Purpose

    Type II Interferon - STAT1/STAT1 gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    STAT1

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. mARGAna (cassette 1, transient)

    Plasmid

    #197588

    Purpose

    Second-generation mammalian acoustic reporter gene derived from Anabaena

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    mARGAna (cassette 1)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. mARGAna (cassette 2, transient)

    Plasmid

    #197589

    Purpose

    Second-generation mammalian acoustic reporter gene derived from Anabaena

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    mARGAna (cassette 2)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLJM1-Empty

    Plasmid

    #91980

    Purpose

    Empty backbone

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET29-bdSUMO-Anti-ALFA nanobody

    Plasmid

    #212311

    Purpose

    Expression of anti-ALFA nanobody

    Depositor

    Arthur Laganowsky

    Article

    Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

    Insert

    anti-ALFA nanobody
    

    Tags

    bdSUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF2546

    Plasmid

    #144209

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CEBPA (CEBPA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTubb3-MC

    Plasmid

    #87112

    Purpose

    minicircle parental backbone plasmid including GFP knock-in donor targeting Tubb3 gene and one cutting site for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    GFP
    

    Use

    CRISPR

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-µLight

    Plasmid

    #201225

    Purpose

    Express muLight biosensor in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

    Insert

    µLight
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJRH-1362 scFv entry plasmid

    Plasmid

    #201912

    Purpose

    Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. ND6-DdCBE-left side TALE

    Plasmid

    #157842

    Purpose

    expresses left-side TALE–DddAtox half of ND6-DdCBE in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    SOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–SOD2 3'UTR
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. nuclear NAD+ biosensor

    Plasmid

    #186789

    Purpose

    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleus

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    nuclear NAD+ biosensor
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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