164,782 results

1. pHR_PGK_SNIPR_CD8alpha variant 4

   Plasmid

   #188364

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant4-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_CD8alpha-variant4-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-nEF-Con/Fon-ChRmine-oScarlet (AAV8)

   Viral Prep

   #137159-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-ChRmine-oScarlet (#137159). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-ChRmine-oScarlet plasmid DNA. nEF-driven, Cre and Flp-dependent expression of ChRmine-oScarlet. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   nEF

   Tags

   oScarlet (Cre and Flp-dependent)

   Available since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

3. pYPQ133A2.0

   Plasmid

   #99891

   Purpose

   Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-UbC-rtTA-Ngn2:2A:EGFP

   Plasmid

   #127288

   Purpose

   all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)

   Depositor

   Fred Gage

   Article

   Schafer et al Nat Neurosci. 2019 Feb;22(2):243-255. do

   Insert

   Ngn2:2A:Ascl1 (NEUROG2 Human)
   

   Use

   Lentiviral

   Tags

   Ngn2 and EGFP linked via 2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR_PGK_SNIPR_CD8alpha variant 2, G318A

   Plasmid

   #188365

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD_G318A, a Notch1-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_G318A_Notch1-JMD_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCPmacroH2A.1-GS3

   Plasmid

   #204759

   Purpose

   Expression of Cas9 and human macroH2A.1

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   macroH2A1 (MACROH2A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-GFP (AAV1 trial size)

   Viral Prep

   #37825-AAV1.T

   Purpose

   Ready-to-use AAV1 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Promoter

   CAG

   Tags

   GFP

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-flex-taCasp3-TEVp

   Plasmid

   #45580

   Depositor

   Nirao Shah, Jim Wells

   Article

   Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

   Has Service

   AAV1 and AAV5

   Inserts

   Caspase 3 (CASP3 Human, Synthetic)
   Tobacco Etch Virus protease
   

   Use

   AAV; Adeno associated viral vector

   Tags

   Expression

   Mutation

   Linker replaced with a TEV protease cleavage site

   Promoter

   EF1a

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. cacna2d1 pCAGGS

   Plasmid

   #206104

   Purpose

   expression of rat alpha2delta-1 calcium channel auxillary subunit

   Depositor

   Annette Dolphin

   Insert

   cacna2d1 (Cacna2d1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/B-actin

   Available since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PRE-Hsp70BbCas9_1.3

    Plasmid

    #190797

    Purpose

    aka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final product

    Depositor

    Omar Akbari

    Article

    Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

    Insert

    2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-XBP1 mNeonGreen NLS

    Plasmid

    #115968

    Purpose

    ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen

    Depositor

    David Andrews

    Article

    Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

    Insert

    X-box binding protein 1 (XBP1 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    HA tag, c-myc NLS, and mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF0139

    Plasmid

    #141533

    Purpose

    Lentiviral vector for overexpressing the UBTF transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    UBTF (UBTF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEAK10-His-Myc-Kal9

    Plasmid

    #25441

    Depositor

    Betty Eipper

    Article

    Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

    Insert

    Kal9
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pEAK10-His-Myc-Kal5

    Plasmid

    #25440

    Depositor

    Betty Eipper

    Article

    Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

    Insert

    Kal5
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

15. B-SpCas9-HF1

    Plasmid

    #126762

    Purpose

    Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-SpCas9-HF1
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A; R661A; Q695A; Q926A; amino acids 1005-1013…

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAVLINK-EF1alpha-5' IQGAP3

    Plasmid

    #236704

    Purpose

    5' AAVLINK plasmid for IQGAP3 expression

    Depositor

    Zhonghua Lu

    Insert

    IQGAP3 (IQGAP3 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    Promoter

    EF1alpha

    Available since

    Aug. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. hSyn1 HRP-TM

    Plasmid

    #231500

    Purpose

    Expressing HRP on the cell surface of neurons

    Depositor

    Baljit Khakh

    Article

    Wu et al Neuron. 2025 Jun 5:S0896-6273(25)00366-6

    Insert

    HRP
    

    Use

    AAV

    Tags

    Igk, HA, Myc, PDGFR-beta transmembrane domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC19_CDC45_dTAG

    Plasmid

    #232733

    Purpose

    Generates CDC45 dTAG C ternimal knock-in cell lines as donor

    Depositor

    Junjie Chen

    Insert

    CDC45 (CDC45 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Synonymous mutation

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX459_MCM2_sgRNA

    Plasmid

    #232730

    Purpose

    Generates MCM2 dTAG C ternimal knock-in cell lines

    Depositor

    Junjie Chen

    Insert

    MCM2 (MCM2 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    NO

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX459_GINS4_gRNA

    Plasmid

    #232734

    Purpose

    Generates GINS4 dTAG C ternimal knock-in cell lines

    Depositor

    Junjie Chen

    Insert

    GINS4 (GINS4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    NO

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge