We narrowed to 26,655 results for: vit;

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POC1473

Plasmid

#213761

Purpose

BpiI-associated switch methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated switch methylase M2.NmeMC58II

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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POC1463

Plasmid

#213141

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M2.Eco31I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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Leu2 2u

Plasmid

#220622

Purpose

empty backbone for shuttle vector construction

Depositor

Marvin Wickens

Article

Preston et al Nat Methods. 2019 May;16(5):437-445. doi

Insert

empty vector (LEU2 Budding Yeast)

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:GFP-CAAX-he1.1:YFP

Plasmid

#184816

Purpose

Used to generate the Tg(QUAS:GFP-CAAX; he1.1:YFP)c631 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:GFP-CAAX-pA; he1.1:CFP

Use

Zebrafish tol2 transgenesis

Tags

GFP-CAAX

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDR274-gsc2-sgRNA

Plasmid

#184818

Purpose

Used to synthesize gRNA targeting exon 2 of the gsc2 gene

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

gsc2-sgRNA

Use

CRISPR; Template for sgrna synthesis

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:NLS-mApple-he1.1:CFP

Plasmid

#184814

Purpose

Used to generate the Tg(QUAS:NLS-mApple; he1.1:CFP)c718 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:NLS-mApple-pA; he1.1:CFP

Use

Zebrafish tol2 transgenesis

Tags

NLS-mApple

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:NLS-GFP-he1.1:CFP

Plasmid

#184815

Purpose

Used to generate the Tg(QUAS:NLS-GFP; he1.1:CFP)c682 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:NLS-GFP-pA; he1.1:CFP

Use

Zebrafish tol2 transgenesis

Tags

NLS-GFP

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-SFFVp-gI-BlastR

Plasmid

#207179

Purpose

Gateway compatible Lentiviral Expression Plasmid SFFVp-IRES-BlastR

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

SFFVp

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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β2AR-PPI-CALPon1.0 (TEVp)

Plasmid

#213407

Purpose

Peroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 2)

Depositor

Wenjing Wang

Article

Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

Insert

HA-arrestin-TEVp

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBJ016_MTB_CoChR_KGC_eGFP_ER2

Plasmid

#209277

Purpose

Ubiquitously expresses CoChR in zebrafish via tol2 transgenesis or in vitro transcription of mRNA

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

CoChR_KGC_eGFP_ER2

Use

Zebrafish expression, in vitro transcription

Promoter

actb2

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBJ035_MTB_gtACR2_KGC_eGFP_ER2

Plasmid

#209278

Purpose

Ubiquitously expresses gtACR2 in zebrafish via tol2 transgenesis or in vitro transcription of mRNA

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

gtACR2_KGC_eGFP_ER2

Use

Zebrafish expression, in vitro transcription

Promoter

actb2

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBJ037_MTB_FR-GECO1c

Plasmid

#209279

Purpose

Ubiquitously expresses FR-GECO1c in zebrafish via tol2 transgenesis or in vitro transcription of mRNA

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

FR-GECO1c

Use

Zebrafish expression, in vitro transcription

Promoter

actb2

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRSF- RPA4 (MSW#716)

Plasmid

#208077

Purpose

Expression of human Replication Protein A 4 in E.coli

Depositor

Marc Wold

Article

Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

Insert

RPA4 (RPA4 Human)

Expression

Bacterial

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pJV0016

Plasmid

#205133

Purpose

Expression of 2xPX-GFP in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

tandem 2xPX domain of SnxA

Use

Dictyostelium expression

Tags

EGFP

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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PB-Blast-Dest-LifeAct-mRuby

Plasmid

#194907

Purpose

To generate LifeAct-mRuby expressing stable cell lines with piggybac transposase co-expression and blastocidin selections.

Depositor

Clare Buckley

Article

Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

Insert

LifeAct

Use

Mouse Targeting

Tags

mRuby

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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UBE2G2 shRNA

Plasmid

#201813

Purpose

siRNA

Depositor

Allan Weissman

Article

Chen et al Proc Natl Acad Sci U S A. 2006 Jan 10. 1

Insert

UBE2G2 (UBE2G2 Human)

Use

RNAi

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT(EF1a no GFP)

Plasmid

#172339

Purpose

mnucTomato

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

monomeric nuclear Tomato
HygR

Use

Lentiviral and Synthetic Biology

Tags

3x SV40 NLS

Expression

Mammalian

Promoter

EF1alpha

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT(EF1a)TetR

Plasmid

#172352

Purpose

Tet Repressor reporter

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

TetR
d2eGFP
HygR

Use

Lentiviral and Synthetic Biology

Tags

3x SV40 NLS

Expression

Mammalian

Promoter

EF1alpha

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pL8N_RB610-HBB_X1

Plasmid

#191521

Purpose

Expression of nuclear ZF-DdCBE in mammalian cells (N-terminal DddAC, Right)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

ZF-DdCBE RB610-HBB X1

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK-EGFP

Plasmid

#196606

Purpose

Dual-Luciferase Reporter vector carrying an EGFP direct sequence

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

GFP

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pB7_9-ND51_v8_X1

Plasmid

#191511

Purpose

Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Left)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

v8 ZF-DdCBE 9-ND51 X1

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pT7PI-Sce ESARC D326A

Plasmid

#195609

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D326A active site mutation

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

Insert

PI-SceI

Expression

Bacterial

Mutation

T3593C, A3601C, T3602G

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCZGY2748

Plasmid

#193331

Purpose

Site specific CRISPR/Cas9 editing of C. elegans Chr I

Depositor

Yishi Jin

Article

Blazie et al Elife. 2021 Jul 29;10. pii: 68336. doi:

Insert

sgRNA for ttTi4348

Tags

None

Expression

Worm

Promoter

U6

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pL6_3xG34-DCAF8L2_X1

Plasmid

#191518

Purpose

Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Right)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

ZF-DdCBE 3xG34-DCAF8L2 X1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pL5N_LT32-HBB_X1

Plasmid

#191520

Purpose

Expression of nuclear ZF-DdCBE in mammalian cells (N-terminal DddAN, Left)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

ZF-DdCBE LT32-HBB X1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pL7_3xG35-EMILIN2_X1

Plasmid

#191517

Purpose

Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Left)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

ZF-DdCBE 3xG35-EMILIN2 X1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pB6_R13-ND51_v8_X1

Plasmid

#191512

Purpose

Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Right)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

v8 ZF-DdCBE R13-ND51 X1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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HySp5-420

Plasmid

#192993

Purpose

Plasmid that encodes for Hydra Sp5

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5

Tags

HA

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE32

Plasmid

#90374

Purpose

SP1 - constitutive enhancer gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

SP1

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE11

Plasmid

#90345

Purpose

SRF - Serum response factor gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

SRF

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge