We narrowed to 25,030 results for: Nes

1. EC64422

   Plasmid

   #191768

   Purpose

   CRISPR mutagenesis in Medicago truncatula

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al Methods Mol Biol. 2023;2653:221-252. doi

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET17_K560_GFP_His

   Plasmid

   #15219

   Depositor

   Ron Vale

   Article

   Woehlke et al Cell. 1997 Jul 25. 90(2):207-16.

   Insert

   Human neuronal kinesin heavy chain (KIF5B Human)
   

   Use

   Tags

   GFP and His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-Tag2B-NCre

   Plasmid

   #106368

   Purpose

   N-terminal part of the split-Cre System

   Depositor

   Johannes Hirrlinger

   Article

   Hirrlinger et al PLoS One. 2009;4(1):e4286. doi: 10.1371/

   Insert

   NCre
   

   Use

   Cre/Lox

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEC3105 (pSpy1C-PxylS2_ffluc)

   Plasmid

   #218512

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   deletion of Plac_mrfp cassette

   Promoter

   PxylS2

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMaCTag-Z07

   Plasmid

   #124790

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeonGreen

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mNeonGreen
   

   Use

   Pcr template

   Tags

   mNeonGreen

   Expression

   Mutation

   Promoter

   None

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMaCTag-Z13

   Plasmid

   #120056

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mCherry

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mCherry
   

   Use

   Pcr template

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLIC-SM-HA

   Plasmid

   #111188

   Purpose

   High affinity HA Tagging of weakly expressed genes (optimized for Toxoplasma gondii)

   Depositor

   Silvia Moreno

   Article

   Hortua Triana et al J Eukaryot Microbiol. 2018 Apr 19. doi:

   Type

   Empty backbone

   Use

   Toxoplasma gondii expression

   Tags

   Spaghetti Monster HA Tag

   Expression

   Mutation

   Promoter

   Endogenous

   Available Since

   Aug. 2, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pSLIK_hygro_BirA

   Plasmid

   #136537

   Purpose

   Lentiviral expression vector for an inducible BirA

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   BirA
   

   Use

   Lentiviral; Destinatioin vector for gateway cloni…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV hUbC-dCas9 KRAB-T2A-GFP

   Plasmid

   #67620

   Purpose

   Co-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized dead Cas9 KRAB T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mutation

   D10A and H840A

   Promoter

   Available Since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-Tag3B-CCre

    Plasmid

    #106369

    Purpose

    C-terminal part of the split-Cre System

    Depositor

    Johannes Hirrlinger

    Article

    Hirrlinger et al PLoS One. 2009;4(1):e4286. doi: 10.1371/

    Insert

    CCre
    

    Use

    Cre/Lox

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMaCTag-P29

    Plasmid

    #120040

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-II

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Strep-II
    

    Use

    Pcr template

    Tags

    Strep-II

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUPD2

    Plasmid

    #68161

    Purpose

    Universal domesticator plasmid for level 0 parts

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV hUbC-dCas9 VP64-T2A-GFP

    Plasmid

    #53192

    Purpose

    Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 VP64 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMC-6-Ruby-9

    Plasmid

    #197740

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    Ruby reporter tricistronic CDS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMaCTag-P21

    Plasmid

    #120032

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 24xSunTag

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    24xSunTag
    

    Use

    Pcr template

    Tags

    24xSunTag

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMaCTag-P20

    Plasmid

    #120031

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 10xSuntag

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    10xSuntag
    

    Use

    Pcr template

    Tags

    10xSuntag

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a_T7-ARG2

    Plasmid

    #106474

    Purpose

    Expresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coli

    Depositor

    Mikhail Shapiro

    Article

    Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

    Insert

    ARG2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. 4xHRE_RE:RedF

    Plasmid

    #178314

    Purpose

    HRE RedFirefly luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    HRE RedFirefly reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRANS_231

    Plasmid

    #91120

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: BeYDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLIC-SM-Myc

    Plasmid

    #111189

    Purpose

    High affinity Myc tagging of weakly expressed genes (optimized for Toxoplasma gondii)

    Depositor

    Silvia Moreno

    Article

    Hortua Triana et al J Eukaryot Microbiol. 2018 Apr 19. doi:

    Type

    Empty backbone

    Use

    Toxoplasma gondii expression

    Tags

    Spaghetti Monster Myc Tag

    Expression

    Mutation

    Promoter

    Endogenous

    Available Since

    Aug. 2, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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