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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 20,297 results for: Sele

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Showing: 3621 - 3640 of 20297 results

  3. pSCAR_Cas9-hygro_GFP

    Plasmid
    #162075
    Purpose
    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance
    Depositor
    Robert Manguso
    Publication
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Inserts
    Cas9-2a-hygromycin B phosphotransferase
    EGFP
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EF1a and sv40
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162075-plasmid-map-sequence-id-325304

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  7. PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B

    Plasmid
    #106309
    Purpose
    The plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.
    Depositor
    Zhe J. Liu
    Publication
    Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    6xflag-RT3-3xHaloTag(MtoL)-H2B (H2BC3 Human)
    Tags
    6xflag-3xHaloTag(MtoL)
    Expression
    Mammalian
    Promoter
    EF1
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    106309-plasmid-map-sequence-id-204684

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  12. mEmerald-mDia2-C-14

    Plasmid
    #54158
    Purpose
    Localization: Cytoskeleton, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mDia2
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    54158-plasmid-map-sequence-id-473139

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  15. Epac-S-H188

    Plasmid
    #170349
    Purpose
    mT2Del_EPACdDEPCD_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mT2Del_EPACdDEPCD_tdcp173Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    170349-plasmid-map-sequence-id-344647

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  16. pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A

    Plasmid
    #154773
    Purpose
    plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Publication
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Y125A in G1PylS, hybrid PylT with A41AA and C55A …
    Promoter
    EF1
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    154773-plasmid-map-sequence-id-310428

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  17. pDEST-CMV 3xFLAG-LC3B-GFP

    Plasmid
    #123107
    Purpose
    Expresses 3xFLAG-LC3B-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B
    Depositor
    Robin Ketteler
    Publication
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    Tags
    3xFLAG and EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    123107-plasmid-map-sequence-id-244008

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Showing: 3621 - 3640 of 20297 results