We narrowed to 12,310 results for: LEA
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Plasmid#187579PurposeExpress FLAG epitope and APEX2-tagged PSPC1 fusion protein in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPSPC1 (PSPC1 Human)
TagsAPEX2-FLAGExpressionMammalianPromoterCMVAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-IRES2-p75NTR-GFP
Plasmid#182947PurposeTo express the rat p75NTR neurotrophin receptor. The gene contains an HA tag at the N-TerminalDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertp75NTR-mGFP
TagsHA tag and mEGFPExpressionMammalianMutationThe leader sequence is from preprotrypinPromoterCAGAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST-SmB'
Plasmid#1842DepositorAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
SND1 (SND1A-c003)
Plasmid#98242PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSND1A (SND1 Human)
TagsHis-6-TEVExpressionBacterialMutationpfam00567:TUDOR 677-799PromoterT7Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3.2mar-GFP
Plasmid#63694Purpose3.2 kb marine armor plate enhancer driving GFP reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3.2 kb marine stickleback armor plate enhancer
TagsEGFPExpressionMammalianPromoterhsp70Available sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEMS1611
Plasmid#29276PurposePleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPle139 (NR2E1 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsIntron-LacZ-NLSAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS1612
Plasmid#29277PurposePleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPle140 (NR2E1 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsIntron-LacZ-NLSAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBJG1 T7-8xHis-OGT D554N
Plasmid#154279PurposeBacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
Tags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationAspartate 554 changed to Asparagine- bacterial co…Available sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8m-p10
Plasmid#218862PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8m
Tags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationA25G F286YPromoterhsp70Available sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pbZIP9:GFP-GUS
Plasmid#172483PurposeGFP-GUS driven by the bZIP9 promoterDepositorAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SpdCas9-LBiT_Strep-Tag-II
Plasmid#198751PurposeExpresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferaseDepositorInsertSpdCas9 - LargeBiT
TagsStrep-tag IIExpressionBacterialMutationD10A, H840A (nuclease inactivating mutations in &…PromoterT7Available sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LYRa11-RBD-8h
Plasmid#161822PurposeMammalian expression plasmid for RBD of bat coronavirus isolate LYRa11DepositorInsertReceptor-binding domain (RBD) of spike protein S
TagsGSG linker and 8his tag and HA leader sequenceExpressionMammalianMutationHuman codon optimized RBD (a.a. T324-D522)PromoterCMVAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
His-ZZ-TEV-SNAPf-DYNC2H1
Plasmid#132524PurposeFull length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragmentsDepositorInsertDYNC2H1 (DYNC2H1 Human)
Tags8x His, Linker-TEV-linker-TEV, Precission cleavag…ExpressionInsectMutationCodon optimised for Sf9 expressionAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3A-L-dcas9-NLS
Plasmid#112214PurposeTargeted DNA methylationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAUseCRISPRTagsmCherryMutationD10A,H840A,N863AAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-CCR5
Plasmid#98949PurposeMammalian expression plasmid for N-terminal FLAG-tagged human CCR5DepositorInsertCCR5 (CCR5 Human)
TagsFLAG (dykdddd) epitope tag and Signal/leader sequ…ExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-YOD1
Plasmid#85663PurposeExpression of human YOD1 with N-terminal mCherry tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertYOD1 (YOD1 Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75236PurposeCRISPR/Cas9 plasmid against human NFATc1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA against NFATc1 (NFATC1 Human)
UseCRISPRAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-shCasp2-IRES-GFP
Plasmid#52061Purposestable knockdown of Caspase-2 in mammalian cellsDepositorAvailable sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFIN-EF1a-GFP:2A:CHER(HA)-WPRE
Plasmid#44597DepositorInsertEF1a-GFP:2A:CHER(HA)
UseLentiviralPromoterhuman elongation factor -1a (pos 2528-3971)Available sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWay21-MKP-1L16A/L17A
Plasmid#13478DepositorInsertMKP-1L16A/L17A (Dusp1 Mouse)
TagsEGFPExpressionMammalianMutationChanged Leucine 16 to Alanine; changed Leucine 1…Available sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only