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pFA6a-kanMX6-P3nmt1-mYFP

Plasmid

#105138

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P3nmt1-mYFP

Tags

3nmt1-mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

3nmt1

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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YCe2030 HC_Kan_bGHpA_p16

Plasmid

#100688

Purpose

part designed to occupy position 16 of EMMA. Functional category: polyadenylation signal sequence

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

bGHpA

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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env8 16nt J1/13

Plasmid

#99832

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 16nt J1/13

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 with 16 nucleotides

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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env8 19nt J1/13

Plasmid

#99834

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 19nt J1/13

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 with 19 nucleotides

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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env8 17nt J1/13

Plasmid

#99833

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 17nt J1/13

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 with 17 nucleotides

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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env8 24nt J1/13

Plasmid

#99835

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 24nt J1/13

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 with 24 nucleotides

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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env8 7U J1/13

Plasmid

#99829

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 7U J1/13

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 converted to 7 U's

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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env8 AC rep J1/13

Plasmid

#99830

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 AC rep J1/13

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 converted to repeating AC's

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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env8 25nt J1/13

Plasmid

#99836

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 25nt J1/13

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 with 25 nucleotides

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pBP-HexHis

Plasmid

#72983

Purpose

Hexahistidine N-terminal tag (5' TAAA/3' CATA fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Polyhistidine (HHHHHH) N-terminal tag

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 RRN3

Plasmid

#67112

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

RRN3 (RRN3 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
CS2-GFP-nopo

Plasmid

#47964

Purpose

Expression of Fly nopo

Depositor

Laura Lee

Article

Merkle et al Development. 2009 Feb;136(3):449-59. doi

Insert

no poles (nopo Fly)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
pST39

Plasmid

#64009

Purpose

original polycistronic cloning vector for plasmid suite (generation I)

Depositor

Song Tan

Article

Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pST44

Plasmid

#64007

Purpose

polycistronic cloning vector for pST44 plasmid suite

Depositor

Song Tan

Article

Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-ncpGCaMP6s

Plasmid

#113674

Purpose

A topological variant of GCaMP6s

Depositor

Robert Campbell

Article

Qian et al Chembiochem. 2018 Jun 22. doi: 10.1002/c

Insert

ncpGCaMP6s

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG mito-GCaMP5G

Plasmid

#105009

Purpose

mitochondrial calcium sensor

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

GCaMP5G

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCITF-KCC2

Plasmid

#61404

Purpose

Mammalian expression vector for human KCC2 under CAG promoter. Coexpression of TdTomato with IRES sequence

Depositor

Franck Polleux

Article

Bortone et al Neuron. 2009 Apr 16;62(1):53-71. doi: 10

Insert

KCC2 (SLC12A5 Human)

Tags

IRES-tdTomato-Flag

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pET29a-YS14

Plasmid

#66890

Purpose

Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)

Depositor

Jung-Hyun Min

Article

Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2.

Inserts

His6-Thrombin-Xenopus laevis histones H2A
Xenopus laevis histones H2B
Xenopus laevis histones H3
Xenopus laevis histones H4-Thrombin

Tags

H2A, H2B, H3, H4, His6 tag, and Thrombin

Expression

Bacterial

Promoter

T7

Available Since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only

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PKD1-F (pCI-PKD1-Flag)

Plasmid

#21369

Depositor

Gregory Germino

Article

Hanaoka et al Nature. . 408(6815):990-4.

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

wild-type sequence*

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pPB_mU6_enh-gRNA_Puro-T2A-BFP

Plasmid

#235571

Purpose

Enhanced guide RNA expression & genomic integration. Target gRNA sequences are cloned in via the BstXI and BlpI sites.

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

sgRNA backbone

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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