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pIG-806_HA-GD2-28z_CAR_RFP-TFAP4

Plasmid

#207493

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

RFP-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-755_HA-GD2-28z_CAR_RFP-JUN

Plasmid

#207494

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

RFP-JUN, HA-GD2-28z_CAR (JUN Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-851_HA-GD2-28z_HA-TFAP4

Plasmid

#207501

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

HA-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-852_HA-GD2-28z_TFAP4-HA

Plasmid

#207502

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4-HA, HA-GD2-28z_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-858_CD19-BBz_CAR_TFAP4

Plasmid

#207503

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4, CD19-BBz_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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anti-NPY/Neuropeptide Y scFv [L115/13] with sortase tag

Plasmid

#204426

Purpose

Mammalian Expression of anti-Neuropeptide Y scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L115/13.

Depositor

Jeff Lichtman

Article

Han et al Nat Commun. 2024 Aug 5;15(1):6648. doi:

Insert

Recombinant mouse scFv targeting NPY/Neuropeptide Y (Homo sapiens)

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pTR-454 tNGFR-P2A-CARD11

Plasmid

#186081

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CARD11 (CARD11 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pNL-CEF-IgG1

Plasmid

#187010

Purpose

Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgG1

Depositor

Hermann Eibel

Article

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgG1

Use

Lentiviral

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMV-Tc1-2_ST-10 kb

Plasmid

#218314

Purpose

Donor plasmid of Tc1-2_ST Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-8B_DR-10 kb

Plasmid

#218318

Purpose

Donor plasmid of Tc1-8B_DR Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Tc1-1_PM-10 kb

Plasmid

#218319

Purpose

Donor plasmid of Tc1-1_PM Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-piggyBac-1_AMi-10 kb

Plasmid

#218320

Purpose

Donor plasmid of piggyBac-1_AMi Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-1_Xt-10 kb

Plasmid

#218316

Purpose

Donor plasmid of Tc1-1_Xt Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-3_FR-10 kb

Plasmid

#218317

Purpose

Donor plasmid of Tc1-3_FR Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pICOZ-MAG[EF1α-CD19 4-1BB]

Plasmid

#218329

Purpose

Donor plasmid of MAG Transposon with CD19 CAR mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

CD19 CAR mammalian expression cassette

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pICOZ-MAG[EF1α-HER1 4-1BB]

Plasmid

#218331

Purpose

Donor plasmid of MAG Transposon with HER1 CAR mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

HER1 CAR mammalian expression cassette

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pICOZ-SB100X[EF1α-CD19 CD28Z]

Plasmid

#218332

Purpose

Donor plasmid of SB100X Transposon with CD19 CAR mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

CD19 CAR mammalian expression cassette

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pICOZ-MAG[EF1α-CD19 CD28Z]

Plasmid

#218333

Purpose

Donor plasmid of MAG Transposon with CD19 CAR mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

CD19 CAR mammalian expression cassette

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Tc1-2_ST-5 kb

Plasmid

#218304

Purpose

Donor plasmid of Tc1-2_ST Transposon with 5 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

5 kb cargo gene

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-1_Xt-5 kb

Plasmid

#218306

Purpose

Donor plasmid of Tc1-1_Xt Transposon with 5 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

5 kb cargo gene

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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