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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,824 results for: nls

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Showing: 3641 - 3660 of 18824 results

  5. CAGGS-EGFP-NLS-3XFLAG-V5

    Plasmid
    #196090
    Purpose
    Expresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag
    Depositor
    Mauro Calabrese
    Publication
    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP
    Tags
    C-terminal 3xFLAG and C-terminal V5
    Expression
    Bacterial and Mammalian
    Promoter
    CAGGs
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    196090-plasmid-map-sequence-id-384636

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  7. pGEX-4T1 NLS-GFP

    Plasmid
    #129441
    Purpose
    bacterial expression of GFP
    Depositor
    Oskar Laur
    Insert
    GFP
    Expression
    Bacterial
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129441-plasmid-map-sequence-id-256454

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  8. XLone-Puro SARS-CoV2 N P2A eGFP-NLS

    Plasmid
    #154399
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and nuclear eGFP
    Depositor
    Xiaoping Bao
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV2 N Protein (N SARS-CoV2)
    Use
    Piggybac
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    154399-plasmid-map-sequence-id-304590

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  10. pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

    Plasmid
    #101102
    Purpose
    Encodes dCas9(C)-VP64 with SAM components fused to transmembrane tether
    Depositor
    Tudor Fulga
    Publication
    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TMt-dCas9(C)-VP64
    Use
    CRISPR and Synthetic Biology
    Tags
    HA and Myc
    Expression
    Mammalian
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    101102-plasmid-map-sequence-id-197199

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  12. pDR111_mannose_Bgal NLS

    Plasmid
    #188398
    Purpose
    Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.
    Depositor
    Christopher Contag
    Publication
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Insert
    lacZ
    Tags
    PhoD secretion peptide and SV40 NLS
    Expression
    Bacterial
    Promoter
    D-mannose
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188398-plasmid-map-sequence-id-370473

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  13. pcDNA3-P4:gs:nls:FKBP

    Plasmid
    #135984
    Purpose
    Expresses the P4 coiled coil fused to the FKBP rapamycin-inducible domain in mammalian cells
    Depositor
    Roman Jerala
    Publication
    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    P4 coiled-coil fused to the FKBP domain
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    135984-plasmid-map-sequence-id-270610

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  14. pcDNA3-His:FRB:nls:VPR

    Plasmid
    #135987
    Purpose
    Expresses the FRB rapamycin-inducible domain fused to the VPR transcriptional activation domain in mammalian cells
    Depositor
    Roman Jerala
    Publication
    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FRB domain fused to the VPR activation domain
    Tags
    His
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    135987-plasmid-map-sequence-id-268598

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  15. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA

    Plasmid
    #134925
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.
    Depositor
    Tomoji Mashimo
    Publication
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134925-plasmid-map-sequence-id-267605

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  16. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA

    Plasmid
    #134922
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.
    Depositor
    Tomoji Mashimo
    Publication
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134922-plasmid-map-sequence-id-267588

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  17. pPV-Dual_promoter-EF1α-2xNLS-Cascade+Cas3-P (RD)

    Plasmid
    #204619
    Purpose
    Expression vector of Cascade and Cas3. Puromycin selectable.
    Depositor
    Akitsu Hotta
    Publication
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas7, Cas5, Cas8, Cas11, Cas6, and Cas3
    Use
    CRISPR
    Tags
    Each protein has C- and N-terminal SV40 NLS
    Promoter
    EF1a
    Available since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    204619-plasmid-map-sequence-id-404756

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  19. TV_EMP1-ires-mNeon-nls-p2a-icasp9-wpre_pgk-puro

    Plasmid
    #251175
    Purpose
    Targeting vector for C-terminal knockin at EMP1 locus to enable reporting of expression by mNeonGreen and depletion of positive cells with iCasp9
    Depositor
    Hugo Snippert
    Insert
    ires-mNeon-nls-p2a-icasp9-wpre_pgk-puro flanked by homology arms for C-terminal targeting of human EMP1
    Use
    CRISPR
    Tags
    ires-mNeon-nls-p2a-icasp9
    Available since
    Feb. 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    251175-plasmid-map-sequence-id-500692

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Showing: 3641 - 3660 of 18824 results