We narrowed to 6,241 results for: nls

1. pKI1.1

   Plasmid

   #85807

   Purpose

   CRISPR/Cas9 for Arabidopsis in pENTR/D-TOPO

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Promoter

   AtRPS5A

   Available Since

   May 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXS-IRES-BLAST co hPHGDH

   Plasmid

   #154915

   Purpose

   Express codon optimized human PHGDH

   Depositor

   Richard Possemato

   Article

   Richard et al Richard Possemato Lab plasmids

   Insert

   PHGDH, codon optimized (PHGDH Synthetic, Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Codon optimized for expression in human cells

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBSKΔB-TRE-dCas9-EGFP

   Plasmid

   #62347

   Purpose

   Plasmid for tet-inducible expression of dCas9-EGFP in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

   Insert

   dCas9-EGFP
   

   Use

   CRISPR

   Tags

   HA and SV40 NLS

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRETightBI-RY-HMGA2wt

   Plasmid

   #31469

   Depositor

   Phil Sharp

   Article

   Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

   Insert

   eYFP and mCherry
   

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pIR190

   Plasmid

   #64812

   Purpose

   TCP4 expression in yeast

   Depositor

   Detlef Weigel

   Article

   Rubio-Somoza et al PLoS Genet. 2013 Mar;9(3):e1003374. doi:

   Insert

   TCP4 (TCP4 Mustard Weed)
   

   Tags

   GAL4 Activation Domain and SV40 NLS

   Available Since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. BCJJ180

   Plasmid

   #117516

   Purpose

   Cas9_4 Golden Gate Level 0

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BsaI:AATG:Cas9_4:GCTT:BsaI
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Plant

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRETightBI-RY-4

   Plasmid

   #31465

   Depositor

   Phil Sharp

   Article

   Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

   Insert

   eYFP and mCherry
   

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pNXRVj-HACre

   Plasmid

   #78407

   Purpose

   constitutive Cre expression vector

   Depositor

   Kazunori Tomita

   Article

   Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

   Inserts

   3xHA-nls-Cre
   aur1R (aur1 Fission Yeast)
   

   Expression

   Yeast

   Mutation

   Gly 240 to Cys

   Available Since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. RHD_exon1_TALEN_R

   Plasmid

   #62240

   Purpose

   expression of TALEN targeting human RHD exon1

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Commun. 2015 Jun 16;6:7451. doi: 10.

   Insert

   human RHD_exon1_TALEN_Right
   

   Use

   TALEN

   Tags

   HA tag, NLS

   Promoter

   CMV

   Available Since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. RHD_exon1_TALEN_L

    Plasmid

    #62239

    Purpose

    expression of TALEN targeting human RHD exon1

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Commun. 2015 Jun 16;6:7451. doi: 10.

    Insert

    human RHD_exon1_TALEN_Left
    

    Use

    TALEN

    Tags

    HA tag, NLS

    Promoter

    CMV

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTRETightBI-RY-1

    Plasmid

    #31464

    Depositor

    Phil Sharp

    Article

    Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

    Insert

    eYFP and mCherry
    

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

12. 6xHis-MBP-huLb5Cas12a

    Plasmid

    #174684

    Purpose

    Expresses 6xHis-MBP tagged Lb5Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huLb5Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTSK368

    Plasmid

    #34551

    Depositor

    James McNally

    Article

    Karpova et al Science. 2008 Jan 25;319(5862):466-9.

    Insert

    MS2-CP-NLS
    

    Tags

    GFP

    Expression

    Yeast

    Promoter

    Met25

    Available Since

    Jan. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTRETightBI-RY-7

    Plasmid

    #31466

    Depositor

    Phil Sharp

    Article

    Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

    Insert

    eYFP and mCherry
    

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pTRETightBI-RY-1pf

    Plasmid

    #31467

    Depositor

    Phil Sharp

    Article

    Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

    Insert

    eYFP and mCherry
    

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pTRETightBI-RY-HMGA2mutant

    Plasmid

    #31470

    Depositor

    Phil Sharp

    Article

    Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

    Insert

    eYFP and mCherry
    

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pCMV-T7-SpG-P2A-mScarlet (MNW006)

    Plasmid

    #174136

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYZ675

    Plasmid

    #132954

    Purpose

    crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a Schizosaccharomyces pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

    Insert

    dFnCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    nucleoplasmin NLS

    Promoter

    adh1

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. 6xHis-MBP-huPcCas12a

    Plasmid

    #174681

    Purpose

    Expresses 6xHis-MBP tagged PcCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPcCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. 6xHis-MBP-huCMtCas12a

    Plasmid

    #174680

    Purpose

    Expresses 6xHis-MBP tagged CMtCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huCMtCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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