Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 6,592 results for: nls

Clear keywords
Showing: 3641 - 3660 of 6592 results

  1. ipUSEPR-sg-Hs-PHGDH-int3

    Plasmid
    #188683
    Purpose
    control sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgPHGDH intron control (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Mutation
    WT
    Available Since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188683-plasmid-map-sequence-id-375016

    Enlarge

  2. pSaCas9_GFP

    Plasmid
    #64709
    Purpose
    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells
    Depositor
    Kiran Musunuru
    Insert
    SaCas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP and 3xFLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    64709-plasmid-map-sequence-id-322473

    Enlarge

  3. Protein2PAM_NmeN4C.1

    Plasmid
    #250218
    Purpose
    Express Protein2PAM_NmeN4C.1 nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeN4C.1
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 E954C/Q981G/F1020Y/A1021S/C1023L/H1024D/…
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250218-plasmid-map-sequence-id-501771

    Enlarge

  4. Protein2PAM_NmeN4G.1

    Plasmid
    #250224
    Purpose
    Express Protein2PAM_NmeN4G.1 nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeN4G.1
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 D957G/V979I/V980K/Q981A/Q989T/N996E/S100…
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250224-plasmid-map-sequence-id-501770

    Enlarge

  5. pCMV-ABE-P48R

    Plasmid
    #161816
    Purpose
    Expresses ABE-P48R in mammalian cells
    Depositor
    Sangsu Bae
    Insert
    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available Since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    161816-plasmid-map-sequence-id-320046

    Enlarge

  6. HaloTag-LacI

    Plasmid
    #232636
    Purpose
    lac repressor (LacI) which binds lacO sites; labeled with HaloTag
    Depositor
    Karsten Rippe
    Insert
    HaloTag-LacI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232636-plasmid-map-sequence-id-462868

    Enlarge

  7. pSBtet-RB-PE2

    Plasmid
    #173903
    Purpose
    SB-transposon with inducible expression of SpCas9 PE2
    Depositor
    Ronald Cohn
    Insert
    PE2
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    173903-plasmid-map-sequence-id-346343

    Enlarge

  8. Protein2PAM_NmeN4CNNT.1

    Plasmid
    #250222
    Purpose
    Express Protein2PAM_NmeN4CNNT.1 nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeN4CNNT.1
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 P1003S/A1014N/A1021V/C1023L/H1024D/G1026…
    Promoter
    CMV
    Available Since
    April 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    250222-plasmid-map-sequence-id-508436

    Enlarge

  9. Protein2PAM_NmeN4G.2

    Plasmid
    #250225
    Purpose
    Express Protein2PAM_NmeN4G.2 nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeN4G.2
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 Y960H/V979I/V980I/Q981A/E1008K/M1016R/N1…
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250225-plasmid-map-sequence-id-501785

    Enlarge

  10. Protein2PAM_NmeN4C.3

    Plasmid
    #250220
    Purpose
    Express Protein2PAM_NmeN4C.3 nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeN4C.3
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 E954C/D957G/Q981G/F1020Y/A1021S/C1023L/H…
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250220-plasmid-map-sequence-id-501769

    Enlarge

  11. Protein2PAM_NmeN4C.2

    Plasmid
    #250219
    Purpose
    Express Protein2PAM_NmeN4C.2 nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeN4C.2
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 E954C/Q981G/F1020Y/A1021S/C1023L/H1024D/…
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250219-plasmid-map-sequence-id-501762

    Enlarge

  12. pSBbi-RB-PE2

    Plasmid
    #173902
    Purpose
    SB-transposon with constitutive expression of SpCas9 PE2
    Depositor
    Ronald Cohn
    Insert
    PE2
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    173902-plasmid-map-sequence-id-346342

    Enlarge

  13. Ubiquiton Yeast Kit

    Plasmid Kit
    #1000000243
    Purpose
    Plasmids for inducible, linkage-specific polyubiquitylation of a protein of interest in yeast.
    Depositor
    Helle Ulrich
    Application
    Gene Expression and Labeling
    Vector Type
    Yeast Expression
    Available Since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only

  17. PX458-3xHA-en31FnCas9

    Plasmid
    #201949
    Purpose
    Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Article
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    3xHA-NLS-en31FnCas9-T2A-EGFP
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    G1243T, E1369R, E1603H on FnCas9
    Promoter
    Cbh
    Available Since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201949-plasmid-map-sequence-id-405303

    Enlarge

  18. pFGC-I2Cas9

    Plasmid
    #173158
    Purpose
    Expresses Cas9 in dividing Arabidopsis cells. Contains dsRED and Basta for fast transformant selection.
    Depositor
    Piotr Ziolkowski
    Article
    Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:
    Inserts
    hSpCas9
    pNAP:dsRED:tNOS
    pMAS:BAR:tMAS
    ICU2 upstream regulatory region
    Tags
    3xFLAG-NLS and NLS
    Expression
    Plant
    Mutation
    Contains a D169N mutation with no visible effect …
    Available Since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    173158-plasmid-map-sequence-id-358594

    Enlarge

Showing: 3641 - 3660 of 6592 results