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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,562 results for: nls

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Showing: 3641 - 3660 of 6562 results

  1. pcDNA3.1-CibN-dCas9

    Plasmid
    #60551
    Purpose
    Expresses CibN-dCas9 in mammalian cells
    Depositor
    Charles Gersbach
    Publication
    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CibN-dCas9
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    inactivating Cas9 mutations D10A and H840A
    Promoter
    CMV
    Available since
    Feb. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    60551-plasmid-map-sequence-id-97050

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  5. pDD3248 pCS2-mtagBFP2-p2a-NES-arrb2a-(ENLYFQ/S)x3-SV40NLS-sfCherry3C(1-10)

    Plasmid
    #251770
    Purpose
    Blue fluorescent protein-tagged TEVp substrate (three-peat motif) for fluorescent protein reconstitution assay
    Depositor
    Dave Dingal
    Publication
    Lim Suan et al Nat Commun 17, 3257 (2026).
    Insert
    arrestin, beta 2a (arrb2a Zebrafish)
    Tags
    NLS (SV40), sfCherry3C large fragment and mTagBFP…
    Expression
    Mammalian
    Promoter
    CMV IE94
    Available since
    April 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    251770-plasmid-map-sequence-id-504794

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  6. pGfa2-nLac

    Plasmid
    #53126
    Purpose
    Expression of LacZ driven by the human Gfa2 promoter. LacZ can also be replaced with the gene of interest for targeting transgene expression to astrocytes in mice.
    Depositor
    Michael Brenner
    Publication
    Brenner et al J Neurosci. 1994 Mar;14(3 Pt 1):1030-7.
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    LacZ, NLS, and mP1
    Expression
    Mammalian
    Mutation
    contains -2163 to +47 (the human gfa2 segment), w…
    Promoter
    human GFAP
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    53126-plasmid-map-sequence-id-82755

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  7. pHS1517

    Plasmid
    #205980
    Purpose
    FLAG-SV40 NLS-Cas5-HNH from MWEE01000013 Cas5-HNH in pcDNA3.1(+)
    Depositor
    Feng Zhang
    Publication
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas5-HNH
    Tags
    FLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205980-plasmid-map-sequence-id-416244

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  8. pHS1862

    Plasmid
    #205975
    Purpose
    FLAG-SV40 NLS-Cas5 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)
    Depositor
    Feng Zhang
    Publication
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas5 from JAGBLH010000220, Cas8-HNH
    Tags
    FLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205975-plasmid-map-sequence-id-416239

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  9. pHS1859

    Plasmid
    #205972
    Purpose
    FLAG-SV40 NLS-Csy3/Cas7 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)
    Depositor
    Feng Zhang
    Publication
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Csy3/Cas7 from JAGBLH010000220, Cas8-HNH
    Tags
    FLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205972-plasmid-map-sequence-id-416242

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  10. pHS1860

    Plasmid
    #205973
    Purpose
    FLAG-SV40 NLS-Cas6/Csy4 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)
    Depositor
    Feng Zhang
    Publication
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas6/Csy4 from JAGBLH010000220, Cas8-HNH
    Tags
    FLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205973-plasmid-map-sequence-id-416241

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  15. pNSEN-d2

    Plasmid
    #59763
    Purpose
    Expresses nuclear EGFP and Tomato in mammalian cells
    Depositor
    Jerry Crabtree
    Publication
    Yoo et al Nature. 2009 Jul 30;460(7255):642-6. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    d2-EGFP
    Tomato
    Tags
    3X NLS and PEST
    Expression
    Mammalian
    Promoter
    CMV promoter and SV40 promoter
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    59763-plasmid-map-sequence-id-94069

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  16. pNuc-TrxRFP1

    Plasmid
    #98998
    Purpose
    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, nuclear localization
    Depositor
    Huiwang Ai
    Publication
    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TrxRFP1 and nuclear localization sequence
    Tags
    His-tag and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    98998-plasmid-map-sequence-id-190366

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  17. INS-GFP-CD19-EPT

    Plasmid
    #210469
    Purpose
    Donor plasmid for knock-in GFP-CD19 into the human INS locus
    Depositor
    Kai Wang
    Publication
    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    INS homologous recombination arms with a GFP-CD19 and EF1alpha-drived puromycin-NLS-tdTomato selection cassette (INS Human)
    Use
    CRISPR
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    210469-plasmid-map-sequence-id-419052

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  19. CAG-xFNLS-T2A-EGFP-ires-puro

    Plasmid
    #121171
    Purpose
    CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    xFNLS-T2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121171-plasmid-map-sequence-id-235381

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  20. pB-EF1a-FastFUCCI-IRES-3xnls-mTagBFP2 (JDW 1511)

    Plasmid
    #242599
    Purpose
    A PiggyBac expression vector containing the human EF1a promoter driving FastFUCCI to label cell cycle state followed by an IRES-3xnls-mTagBFP2.
    Depositor
    Joshua Wythe
    Publication
    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043
    Insert
    mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110) (EEF1A1 Human)
    Expression
    Mammalian
    Promoter
    human EF1a
    Available since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242599-plasmid-map-sequence-id-495380

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Showing: 3641 - 3660 of 6562 results