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pEN_TmiR_G13-L_G12-E

Plasmid

#25763

Purpose

Entry vector with TRE promoter driving both mouse G alpha 12 and G alpha 13 miR30-based shRNAs.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 13 and 12 miR-shRNA

Use

Gateway Cloning

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pSLIK-Venus-TmiR-G12

Plasmid

#25740

Purpose

Lentiviral vector with Tet-based inducible expression of mouse G alpha 12 miR30-based shRNA, constitutive Venus fluorescent protein coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 12 miR-shRNA (Gna12 Mouse)

Use

Lentiviral and RNAi

Tags

Venus

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pSLIK-Venus-TmiR-G13

Plasmid

#25741

Purpose

Lentiviral vector with Tet-based inducible expression of mouse G alpha 13 miR30-based shRNA, constitutive Venus fluorescent protein coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 13 miR-shRNA

Use

Lentiviral and RNAi

Tags

Venus

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pSLIK-Neo-TmiR-Gb2

Plasmid

#25744

Purpose

Control lentiviral vector with Tet-based inducible expression of mouse G beta 2 miR30-based shRNA, constitutive Neomycin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 (Gnb2 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pEN_hU6miR-G13-L

Plasmid

#25758

Purpose

Entry vector with human U6 promoter driving mouse G alpha 13 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 13 miR-shRNA

Use

Gateway Cloning

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pDL560

Plasmid

#231161

Purpose

T-DNA encoding TRV2 with ipt and mobile gRNA targeting SlPDS

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Insert

ipt and mobile gRNA targeting SlPDS

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDL691

Plasmid

#231166

Purpose

T-DNA encoding TRV2 with ipt and mobile gRNAs targeting SlUGT75C1 and SlHAIRLESS

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Insert

ipt and mobile gRNAs targeting SlUGT75C1 and SlHAIRLESS

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-ArgiNLS-mKate2

Plasmid

#220607

Purpose

Flp-dependent expression of a single-cell discriminating version of mKate2 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

mKate2

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-mScarlet-SARS-CoV-2-SL1 alone

Plasmid

#191485

Purpose

Lentiviral expression of SARS-CoV-2 5_ UTR SL1 alone

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

SARS-CoV-2 5' UTR SL1 alone (NEWENTRY Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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SETD2(SET)-Cas9

Plasmid

#186700

Purpose

Plasmid encoding SETD2-Cas9 fusion under CMV promoter

Depositor

Jennifer Doudna

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

Insert

SETD2(SET)-Cas9

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18

Plasmid

#172316

Purpose

CRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAs

Depositor

Steven Jacobsen

Article

Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pGC(2.1)-U6.3

Plasmid

#170516

Purpose

PCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFP

Depositor

Chun Han

Article

Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

Insert

gRNAcore(2.1)-pU6.3

Use

Pcr template vector for amplifying grnacore(2.1)-…

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CAG-NanoLuc-VChR1-EYFP

Plasmid

#114112

Purpose

fusion protein of NanoLuc, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

NanoLuc; Volvox channelrhodopsin 1; EYFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ERBB2 G309E

Plasmid

#40990

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

G309E

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
pACT2-HsMCU-Flag

Plasmid

#253496

Purpose

Flag tagged HsMCU in pACT2 vector

Depositor

Vamsi Mootha

Article

Kovacs-Bogdan et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

HsMCU CD (MCU Human)

Tags

Flag

Expression

Bacterial and Yeast

Available Since

May 18, 2026

Availability

Academic Institutions and Nonprofits only

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co LuxDEC(KZK)

Plasmid

#253533

Purpose

Express luciferin biosynthesis of human codon-optimized LuxDEC in mammalian cells

Depositor

Takeharu Nagai

Article

Kusuma et al Proc Natl Acad Sci U S A. 2024 Oct 8;121

Insert

co LuxD-P2A-co LuxE-T2A-co LuxC

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2026

Availability

Academic Institutions and Nonprofits only

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co LuxB-Frp (G4S)

Plasmid

#253532

Purpose

Express human codon-optimized of LuxB-G4S-Frp in mammalian cells

Depositor

Takeharu Nagai

Article

Kusuma et al Proc Natl Acad Sci U S A. 2024 Oct 8;121

Insert

co LuxB-Frp(G4S)

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2026

Availability

Academic Institutions and Nonprofits only

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pRsetB-TEV-PiTq

Plasmid

#248768

Purpose

Bacterial expression vector for PiTq

Depositor

Gary Yellen

Article

Rosen et al Proc Natl Acad Sci U S A. 2026 Mar 31;12

Insert

PiTq

Expression

Bacterial

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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pPT24-mtArg8m-V5

Plasmid

#251979

Purpose

For biolistic transformation to express Arg8m from mtDNA

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

mtArg8m

Tags

V5

Expression

Yeast

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pPT24-mtArg8m*-V5

Plasmid

#251978

Purpose

For biolistic transformation to express Arg8m* from mtDNA

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

mtArg8m*

Tags

V5

Expression

Yeast

Mutation

premature stop CAA->TAA, Q79*

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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