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pZA58

Plasmid

#174771

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-D481V-4xMyc, Kmr plant selection marker

Tags

4xMyc

Expression

Plant

Mutation

D481V

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pZA52

Plasmid

#174765

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E/D481V with STOP codon

Use

Unspecified

Mutation

L17E/D481V + STOP codon

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pZA50

Plasmid

#174763

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-D481V with STOP codon

Use

Unspecified

Mutation

D481V + STOP codon

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pZA51

Plasmid

#174764

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E with STOP codon

Use

Unspecified

Mutation

L17E + STOP codon

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pZA49

Plasmid

#174762

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_synthesized NbZAR1 (NbZAR1syn) with STOP codon

Use

Unspecified

Mutation

WT + STOP codon

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pZA63

Plasmid

#174776

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E-6xHA, Kmr plant selection marker

Tags

6xHA

Expression

Plant

Mutation

L17E

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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Vimentin-DiB-RM

Plasmid

#168783

Purpose

Vimentin - DiB-RM fusion protein

Depositor

Jens Meiler

Article

Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

Insert

Vimentin-DiB-RM

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc318

Plasmid

#161941

Purpose

Carries Golden Gate substitution insert for ER-LBD domain-insertion mapping

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB1A3-BbsI-G-ER-LBD-S-SapI

Expression

Bacterial

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI743 dual-barcoded gRNA parent vector

Plasmid

#164915

Purpose

CEN/ARS Parent vector for constructing barcoded-gRNA CiBER-seq plasmid libraries

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI733 pRPR1(TetO)-RPC31-sgRNA

Plasmid

#164913

Purpose

For yeast genomic integration of sgRNA against RPC31

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

pRPR1(TetO)-RPC31-sgRNA

Use

CRISPR

Expression

Yeast

Promoter

RPR1

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI732 pRPR1(TetO)-HTS1-sgRNA

Plasmid

#164912

Purpose

For yeast genomic integration of sgRNA against HTS1

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

pRPR1(TetO)-HTS1-sgRNA

Use

CRISPR

Expression

Yeast

Promoter

RPR1

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-HypaCas9-D1135E

Plasmid

#154930

Purpose

aTc inducible HypaCas9 with D1135E mutation

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

HypaCas9-D1135E

Use

CRISPR and Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

N692A/M694A/Q695A/H698A/D1135E

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9HF1-D1135E

Plasmid

#154924

Purpose

aTc inducible Cas9HF1 with D1135E mutation

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

Cas9HF1-D1135E

Use

CRISPR and Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A/D1135E and stop codon on …

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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Integrase triple controller

Plasmid

#126540

Purpose

Pben-int5; Ptet-bxb1; Pbad-tp901: colE1 Chlo+

Depositor

Jerome Bonnet

Article

Zuniga et al Nat Commun. 2020 Sep 21;11(1):4758. doi:

Insert

BenR_pBEN-int5_pTet-bxb1_AraC_pBAD-tp901-1

Expression

Bacterial

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr_2pabPAC

Plasmid

#158971

Purpose

2P-activatable bPAC for lentivirus

Depositor

Michiyuki Matsuda

Article

Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

Insert

2pabPAC

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pZA22

Plasmid

#158500

Purpose

In planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Expression

Plant

Mutation

D481V

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pZA45

Plasmid

#158523

Purpose

In planta gene expression of Golden gate compatible N. benthamiana ZAR1-eGFP. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-eGFP

Tags

eGFP

Expression

Plant

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pZA37

Plasmid

#158515

Purpose

In planta gene expression of Golden gate compatible N. benthamiana ZAR1-6xHA. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-6xHA

Tags

6xHA

Expression

Plant

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pZA21

Plasmid

#158499

Purpose

In planta gene expression of Golden gate compatible N. benthamiana ZAR1. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Expression

Plant

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pZA29

Plasmid

#158507

Purpose

In planta gene expression of Golden gate compatible N. benthamiana ZAR1-4xMyc. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-4xMyc

Tags

4xMyc

Expression

Plant

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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