We narrowed to 11,256 results for: NSI

1. pHH0103_ARHGAP12-1/1

   Plasmid

   #91282

   Purpose

   Protein expression and purification of human SH3 domain construct ARHGAP12-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   ARHGAP12-1/1 (ARHGAP12 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHH0103_AHI1-1/1

   Plasmid

   #91269

   Purpose

   Protein expression and purification of human SH3 domain construct AHI1-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   AHI1-1/1 (AHI1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CiDr-VSP L223F/C302S-mCherry pcDNA3.1 (eVSP CSmutant)

   Plasmid

   #140893

   Purpose

   Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cel

   Depositor

   Yasushi Okamura

   Article

   Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1

   Insert

   CiDr-VSP L223F/C302S-mCherry (vsp, tpte Ciona intestinalis, Zebrafish)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   changed 223L to F in Dr-VSP (zebrafish TPTE), cha…

   Promoter

   Available since

   May 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAG8Ha-SERBP1(149-400)

   Plasmid

   #176516

   Purpose

   Expresses the human C-terminal SERBP1 149-400 construct.

   Depositor

   David Libich

   Article

   Baudin et al Front Mol Biosci. 2021 Sep 24;8:744707.

   Insert

   SERBP1 149-400 (SERBP1 Human)
   

   Use

   Tags

   His8 tag, linker, TEV site

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-148 and 401-408

   Promoter

   T7

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGL3-1574/+120

   Plasmid

   #35539

   Depositor

   Greg Goodall

   Article

   Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

   Insert

   promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   Promoter

   none

   Available since

   June 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHH0103_ABL1-1/1

   Plasmid

   #91521

   Purpose

   Protein expression and purification of human SH3 domain construct ABL1-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   ABL1-1/1 (ABL1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHH0103_ARHGEF9-1/1

   Plasmid

   #91417

   Purpose

   Protein expression and purification of human SH3 domain construct ARHGEF9-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   ARHGEF9-1/1 (ARHGEF9 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN- Incenp-GFP

   Plasmid

   #108484

   Purpose

   inducible expression of vsv-CenpB-1-166-dCEN-INCENP-EGFP

   Depositor

   Susanne Lens

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   CenpB-dCEN-INCENP (INCENP Human)
   

   Use

   Tags

   EGFP and VSV

   Expression

   Mammalian

   Mutation

   CEN box replaced by CenpB 1-166

   Promoter

   CMV

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTRIP-SFFV-mtagBFP-2A RIGI K270A

   Plasmid

   #167290

   Purpose

   Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving catalytically dead (K270A) RLR sensor RIG-I

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   DDX58 (RIGI Human)
   

   Use

   Lentiviral

   Tags

   tagBFP

   Expression

   Mammalian

   Mutation

   K270A

   Promoter

   SFFV

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mCherry-hPMCA2xb

    Plasmid

    #47751

    Purpose

    mammalian expression of mCherry tagged hPMCA2, xb splice variant

    Depositor

    Emanuel Strehler

    Article

    Paszty et al Sci Signal. 2015 Feb 17;8(364):ra19. doi

    Insert

    PMCA2x/b (ATP2B2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    xb splice variant

    Promoter

    CMV

    Available since

    Nov. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1 Flag RdgBbeta (long form) splice variant 1

    Plasmid

    #58275

    Purpose

    mammalian expression of human RdgBbeta splice variant 1

    Depositor

    Shamshad Cockcroft

    Article

    Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

    Insert

    RdgBbeta (PITPNC1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pDisplay-GRAB_CRF0.5

    Plasmid

    #208654

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDEST40-2XHA-Src-K298M-Y419F-Y530F

    Plasmid

    #140320

    Purpose

    Expresses HA-tagged human Src-K298M-Y419F-Y530F in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    K298M-Y419F-Y530F

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PCRII apoEb

    Plasmid

    #121901

    Purpose

    Plasmid for making zebrafish apoEb in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    Apoeb (apoeb Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR221 - myc ULK1 wt

    Plasmid

    #27623

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    mouse myc ULK1 (Myc Mouse)
    

    Use

    Gateway donr vector

    Tags

    myc

    Expression

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Promoter

    Available since

    Feb. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pGL3-SV40P-Tet2 enhancer H1

    Plasmid

    #63883

    Purpose

    Luciferase reporter plasmid used to study transcriptional control of murine Tet2

    Depositor

    Kian Peng Koh

    Article

    Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

    Insert

    Tet2 enhancer fragment H1
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28-MRPP3-His

    Plasmid

    #67866

    Purpose

    bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tag

    Depositor

    Walter Rossmanith

    Article

    Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

    Insert

    MRPP3 (KIAA0391 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino acid 46 of recombinant MRPP3 is preceded by…

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL-p21UTRm1&2

    Plasmid

    #20880

    Depositor

    Robert Blelloch

    Article

    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

    Insert

    p21 3'UTR site 1 and 2 mutation (Cdkn1a Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Predicted miRNA binding site 1 (Position ~420) GC…

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pYPQ239-RR

    Plasmid

    #108862

    Purpose

    FnCpf1 Gateway entry plasmid with N623R and K687R mutations

    Depositor

    Yiping Qi

    Article

    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

    Insert

    FnCpf1-RR (FnCpf1 with N623R and K687R mutations)
    

    Use

    CRISPR; Gateway compatible cpf1 entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    N623R and K687R, Cpf1 is rice codon optimized

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21b-LOV-ipaA L514K L531E

    Plasmid

    #40253

    Depositor

    Brian Kuhlman

    Article

    Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.

    Insert

    LOV-ipaA L514K L531E
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L514K; L531E; Jα helix region hybridized (AsLOV2 …

    Promoter

    T7

    Available since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

21. Nsp14D-mCherry

    Plasmid

    #165139

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp14D (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Nsp4 deltaC-EGFP

    Plasmid

    #165126

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp4 deltaC (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEGB 35S:dCas9:Tnos (GB1191)

    Plasmid

    #68223

    Purpose

    Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed; human codon optimis…

    Promoter

    35S

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMS04 [myo-3p::bPGC::SL2::mCherry]

    Plasmid

    #168172

    Purpose

    Expression of bPGC in BWMs of C. elegans

    Depositor

    Alexander Gottschalk

    Article

    Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

    Insert

    bPGC
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1(-)-V5.hOr47a

    Plasmid

    #126474

    Purpose

    Human codon-optimized version of D. melanogaster Or47a (hOr47a) without N-terminal tags for improved intracellular trafficking in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Insert

    Or47a (Or47a Fly)
    

    Use

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens

    Promoter

    CMV

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. RCASBP(A)-3xFlag-mHoxd13+7Ala

    Plasmid

    #145289

    Purpose

    Avian Expression of murine SPDH (+7Ala) Hoxd13 with N-terminal 3xFlag tag

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    Hoxd13(+7Ala) (Hoxd13 Mouse)
    

    Use

    Viral avian expression

    Tags

    3xFlag

    Expression

    Mutation

    A-repeat expanded

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGL3-SV40P-Tet1 enhancer E

    Plasmid

    #63880

    Purpose

    Luciferase reporter plasmid used to study transcriptional control of murine Tet1

    Depositor

    Kian Peng Koh

    Article

    Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

    Insert

    Tet1 enhancer fragment E
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHH0103_ARHGEF6-1/1

    Plasmid

    #91515

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGEF6-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGEF6-1/1 (ARHGEF6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDEST27-GST-ATXN1 FL(92Q)-S776D

    Plasmid

    #21757

    Depositor

    Huda Zoghbi

    Article

    Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 92 Q(Glutamines) and…

    Promoter

    CMV

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pHR-HOXD13-IDR-YFP-NLS

    Plasmid

    #145277

    Purpose

    Mammalian Expression of Nuclear HOXD13 IDR-YFP with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13-IDR (HOXD13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. GFP-AuroraB L154G/H250Y

    Plasmid

    #108492

    Purpose

    expression of EGFP-AuroraB Analog sensitive (L154G/H250Y)

    Depositor

    Susanne Lens

    Article

    Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

    Insert

    AuroraB (AURKB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Analog sensitive L154G/H250Y

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMXs-hGLIS1GR

    Plasmid

    #59312

    Purpose

    Forced expression of human GLIS1GR

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

    Insert

    GLIS1 (GLIS1 Human)
    

    Use

    Retroviral

    Tags

    cGR - hormone binding domain of glucocorticoid re…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDEST40-2XHA-Src-S32

    Plasmid

    #140308

    Purpose

    Expresses HA-tagged human Src-S32 in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    contains only the SH3 and SH2 domains

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHH0103_CRK-2/2-SV1/2

    Plasmid

    #91319

    Purpose

    Protein expression and purification of human SH3 domain construct CRK-2/2-SV1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    CRK-2/2-SV1/2 (CRK Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSS02:cyt-Peredox-mCherry

    Plasmid

    #161743

    Purpose

    cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

    Depositor

    Markus Schwarzländer

    Article

    Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

    Insert

    cyt-Peredox-mCherry
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Ubiquitin 10

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEGFP-N3/hArf5(WT)-EGFP

    Plasmid

    #79534

    Purpose

    Expresses C-terminally EGFP-tagged Arf5(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf5 (ARF5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHH0103_SRC-1/1-IS2/2

    Plasmid

    #91251

    Purpose

    Protein expression and purification of human SH3 domain construct SRC-1/1-IS2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SRC-1/1-IS2/2 (SRC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAR69_STRI_m(KS_MAT)_H8_pET22b

    Plasmid

    #122849

    Purpose

    Expresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    m(KS_MAT) (Fasn Mouse)
    

    Use

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Nsp13 deltaN1-EGFP

    Plasmid

    #165127

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp13 deltaN1 (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Nsp13 deltaN2-EGFP

    Plasmid

    #165128

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp13 deltaN2 (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge