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Plasmid#177574PurposeMammalian Expression Plasmid of anti-GABA(A)R, Alpha1 (Human). Derived from hybridoma N95/35.DepositorInsertanti-GABA(A)R, Alpha1 (Homo sapiens) recombinant mouse monoclonal antibody (GABRA1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pCEP4-FLAG-CCR5
Plasmid#98949PurposeMammalian expression plasmid for N-terminal FLAG-tagged human CCR5DepositorInsertCCR5 (CCR5 Human)
UseTagsFLAG (dykdddd) epitope tag and Signal/leader sequ…ExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE132
Plasmid#118486Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-dCas9-GFP
Plasmid#64104PurposeExpression of Sp dCas9-GFP in mammalian cellsDepositorInsertSp dCas9
UseCRISPR and LentiviralTagssfGFPExpressionMammalianMutationPromoterEFSAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
opto-RhoGEF-mCherry_pcDNA3.1
Plasmid#164473PurposeExpresses opto-RhoGEF (BcLOV4-GGGSx2-ARHGEF11dh-GGGSx2-mCherry) in a pcDNA3.1 backbone.DepositorInsertopto-RhoGEF
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTol2-nbt-mTagBFP2-P2A-mScarlet
Plasmid#196115Purposezebrafish expression of nbt-mTagBFP2-P2A-mScarletDepositorInsertnbt-mTagBFP2-P2A-mScarlet
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-RAPGEF2
Plasmid#110162PurposeExpression of human RAPGEF2 in mammalian cellsDepositorInsertRAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas-dCas12m
Plasmid#192275PurposeEncodes MmdCas12m (D485A) under a constitutive promoterDepositorInsertMmdCas12m
UseCRISPRTagsExpressionBacterialMutationD485APromoterPJ23108Available sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hDlx-Flex-GFP-Fishell_6 (AAV Retrograde)
Viral Prep#83895-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hDlx-Flex-GFP-Fishell_6 (#83895). In addition to the viral particles, you will also receive purified pAAV-hDlx-Flex-GFP-Fishell_6 plasmid DNA. mDlx-driven, Cre-dependent expression of GFP. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterhDlxTagsGFPAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
LIC TAL Effector Assembly Kit
Plasmid Kit#1000000023PurposeLIC (ligation-independent cloning) TALE repeat unit library of 2-mer fragments for assembly in an expression ready TALEN construct in 2 hierarchical assembly steps. Semiâautomated TALE generationDepositorAvailable sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag Rab12 T56N
Plasmid#86789Purposemammalian expression of Rab12 T56N, constitutively GDP-bound mutantDepositorInsertRab12 (RAB12 Human)
UseTagsFlagExpressionMammalianMutationT56N, constitutively GDP-bound mutantPromoterCMVAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_EGFP
Plasmid#25899DepositorInsertEGFP
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-Stat5
Plasmid#139832Purposedoxycycline-inducible expression of mouse Stat5 in mammalian cellsDepositorInsertStat5 (Stat5a Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE-mCMVAvailable sinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496) (AAV PHP.eB)
Viral Prep#214858-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496) (#214858). In addition to the viral particles, you will also receive purified AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496) plasmid DNA. minBG-driven expression of SYFP2 plus a separate nuclear FLAG epitope in striatal cholinergic neurons. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.PromoterTagsSYFP2Available sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-BBS2
Plasmid#218712PurposeExpresses N-terminally EGFP-tagged BBS2 in mammalian cellsDepositorInsertBBS2 (BBS2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2
Plasmid#218205PurposeZebrafish -2.1prox1a enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1(nsp8-nsp7)(nsp12)
Plasmid#165451PurposeExpresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia ColiDepositorInsertsnsp12
nsp8
nsp7
UseTags14Histidine-TEVExpressionBacterialMutationPromoterT7Available sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-hSOX10
Plasmid#24749DepositorInsert(sex determining region Y)-box 10 (SOX10 Human)
UseGateway donor vectorTagsExpressionMutationwildtype sequencePromoterAvailable sinceMay 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP
Plasmid#118156PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-EGFP
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only