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dPspCas13b-wtFTO

Plasmid

#223696

Purpose

Expressing fusion protein of dPspCas13b and wild type human FTO

Depositor

Chuan He

Article

Wei et al Science. 2022 May 27;376(6596):968-973.

Insert

dPspCas13b-GGS-wtFTO (FTO Human)

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pST_140_LVL2 cam

Plasmid

#179334

Purpose

NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout

Use

CRISPR

Mutation

Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC30A7_STOP

Plasmid

#161314

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC30A7 (SLC30A7 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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TRE:mCherry-p2a- bReaChES

Plasmid

#163037

Purpose

FLiCRE reporter gene for neuronal expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

mCherry-p2a-bReaChES

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-TfR-mApple

Plasmid

#179384

Purpose

Lentiviral vector for expression of Human Transferrin receptor-1 fused mApple

Depositor

Angus Johnston

Article

Rennick et al Nat Commun. 2022 Oct 12;13(1):6023. doi:

Insert

Transferrin receptor 1 - mApple (TFRC Synthetic, Human)

Use

Lentiviral

Tags

mApple

Expression

Mammalian

Promoter

EF1

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II

Plasmid

#52092

Purpose

Retroviral expression of all four WT CD3 subunits, co-expressed with EGFP

Depositor

Dario Vignali

Article

Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

Inserts

CD3 delta (Cd3d Mouse)
CDC gamma (Cd3g Mouse)
CD3 epsilon (Cd3e Mouse)
CD3 zeta (Cd247 Mouse)

Use

Retroviral

Tags

F2A, IRES-EGFP, P2A, and T2A

Expression

Mammalian

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV_CK8e_Cas9_miniPA

Plasmid

#226141

Purpose

AAV construct for HITI insert with CK8e promoter and SpCas9

Depositor

Christopher Nelson

Article

Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

Insert

Cas9

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CK8e

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV

Plasmid

#24828

Depositor

Lin He

Article

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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AdEasier-1 cells (strain)

Bacterial Strain

#16399

Depositor

Bert Vogelstein

Article

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Bacterial Resistance

Ampicillin and Streptomycin

Species

Escherichia coli

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3/er-(n2)oxStayGold(c4)v2.0

Plasmid

#186296

Purpose

A new version of er-(n2)oxStayGold(c4), which gives a higher expression level. The StayGold gene has codon optimization for mammalian expression. For brighter ER imaging if permitted.

Depositor

Atsushi Miyawaki

Article

Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

Insert

er-(n2)oxStayGold(c4)v2.0

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLXSN18E6E7

Plasmid

#53459

Purpose

The plasmid, pLXSN18E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV18 E6 and E7 genes.

Depositor

Denise Galloway

Article

Halbert et al J Virol. 1991 Jan;65(1):473-8.

Insert

HPV18E6E7

Use

Retroviral

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only

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pFB2E

Plasmid

#195288

Purpose

Bicistronic HSV-TK-EGFP fusion and Puromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK EGFP 2A Puro

Expression

Mammalian

Promoter

EFS

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pET28 His6-mEGFP-D4H

Plasmid

#226373

Purpose

Production of recombinant mEGFP-D4H, a fluorescent probe binding cholesterol

Depositor

Catherine Tomasetto

Article

Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

Insert

D4H (pfoA Clostridium perfringens)

Tags

His6 and monomeric EGFP

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pPSU20-40-60-80

Plasmid

#173839

Purpose

Coexpresses 20, 40, 60 and 80 kD Penn State ladder proteins

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABPACCBPv3

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet-I3

Plasmid

#189757

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-I3 red fluorescent protein

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I3

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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NM9-SpCas9-NLS3

Plasmid

#128177

Purpose

Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

Depositor

Huimin Zhao

Article

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

SpCas9-NLS3

Use

CRISPR

Expression

Yeast

Promoter

pPGK1

Available Since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-pBAD

Plasmid

#54572

Purpose

Localization: Protein Expression Vector , Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

6xHis, T7, and mTagBFP2

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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V5-GFP-TurboID-KDEL_pLX208

Plasmid

#202020

Purpose

expresses GFP-TurboID in the mammalian ER lumen, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-TurboID-KDEL

Use

Lentiviral

Tags

GFP and V5

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-CaM-uTEVp

Plasmid

#163032

Purpose

FLiCRE protease for neuronal expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

GFP-CaM-uTEVp

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAG415GAL-ccdB

Plasmid

#14145

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Expression

Yeast

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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