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PJ14

Plasmid

#25912

Depositor

Kenneth Marcu

Publication

Lang et al Nucleic Acids Res. 1982 Jan 22. 10(2):61

Insert

Ig germline mu switch region

Expression

Bacterial

Available since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pBlue-hFX-HA

Plasmid

#14987

Depositor

Michael Ristow

Publication

Shoichet et al Hum Mol Genet. 2002 Apr 1. 11(7):815-21.

Insert

human frataxin cDNA (FXN Human)

Tags

AU1 and HA

Expression

Bacterial

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pPS1358

Plasmid

#8918

Depositor

Pamela Silver

Publication

Kessler et al Genes Dev. 1997 Oct 1. 11(19):2545-56.

Insert

HRP1 (HRP1 Budding Yeast)

Tags

GFP

Expression

Yeast

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only

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pPROBE'-gfp[LVA]

Plasmid

#40171

Depositor

Steven Lindow

Publication

Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

Promotor probe

Available since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-pCALM1-sFLEx-HA-SpCas9-miniU6-sgRNAShank3

Plasmid

#213969

Purpose

AAV vector for encoding SpCas9 driven by pCALM1 promoter targeting Shank3 locus in the presence of Cre recombinase

Depositor

Zhonghua Lu

Publication

Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

Insert

Shank3 sgRNA

Use

AAV and CRISPR

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SF-iGluSnFR.A184V

Plasmid

#106193

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Mutation

GltI: A184V

Promoter

GFAP

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-fd [Chronos-GFP]

Plasmid

#84482

Purpose

AAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal.

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

human synapsin promoter

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-Syn-FLEX-rc [Chronos-tdTomato]

Plasmid

#84481

Purpose

AAV-mediated expression of Chronos-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Promoter

human synapsin promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCSIIpuro-NRG1-ScNeo

Plasmid

#209900

Purpose

To monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Publication

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

NRG1-ScNeo (NRG1 Human)

Use

Lentiviral

Tags

mNeonGreen and mScarlet

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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iRFP713-N2-Frankenbody-Flag

Plasmid

#175291

Purpose

Visualize endogeneous loci coupled with ZF probes harboring 10xFLAG tag

Depositor

Tim Stasevich

Publication

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with iRFP713

Tags

iRFP713

Expression

Mammalian

Available since

Dec. 3, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-CaMKII-GRAB_eCB2.0

Plasmid

#164608

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in CaMKII positive neurons

Depositor

Yulong Li

Publication

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Use

AAV

Mutation

S383T

Promoter

CaMKII

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-Hygro-sgSIK3

Plasmid

#138699

Purpose

Expresses a human SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Publication

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 human (SIK3 Human)

Use

Lentiviral

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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JPF0419d

Plasmid

#124020

Purpose

Encodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pCAG-Lyn-iRFP713-P2A-NES-mAzamiGreen-P2A-p38KTR-mCerulean-P2A-H2B::mScarlet

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_032

Plasmid

#123995

Purpose

Encodes the spCas9 sgRNA1 hAAVS1 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA1 -hAAVS1

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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shCCL20

Plasmid

#65096

Purpose

retroviral expression of CCL20 shRNA

Depositor

Pau Sancho-Bru

Publication

Affo et al Gut. 2014 Nov;63(11):1782-92. doi: 10.11

Insert

Ccl20 shRNA (Ccl20 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

U6

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pGST2-yVps23-UEV_C133A

Plasmid

#45924

Depositor

James Hurley

Publication

Ren et al EMBO J. 2011 Jun 1;30(11):2130-9. doi: 1

Insert

yeast Vps23-UEV(1-160) (STP22 Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

C133A

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pCAG-postASAP

Plasmid

#178792

Purpose

Expresses voltage sensor targeted to dendrites and spines

Depositor

Rafael Yuste

Publication

Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

Insert

FingR.PSD95 and ASAP2f

Expression

Mammalian

Mutation

mutations in amino acids L146G, S147T N149R, S150…

Promoter

CAG

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pαH-S-RRAR-OMICRON

Plasmid

#180424

Purpose

Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side intact

Depositor

Priyamvada Acharya

Publication

Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

Insert

Spike (S-RRAR-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)

Tags

HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPROBE-gfp[ASV]

Plasmid

#40166

Depositor

Steven Lindow

Publication

Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

Promotor probe

Available since

Dec. 6, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pADH99Cau

Plasmid

#244807

Purpose

Modified plasmid from pADH99 to perform HIS-FLP CRISPR in Candidozyma auris

Depositor

Patrick Van Dijck

Publication

Sofras et al bioRxiv [Preprint]. 2025 Jan 11:2025.01.

Insert

CauHIS1_US

Use

CRISPR

Tags

C. albicans MAL2 promoter, C. albicans codon opti…

Expression

Yeast

Available since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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