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pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2)

Plasmid

#169161

Purpose

To express nsp10-nsp14 fusion protein in E. coli

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

14His-SUMO-nsp10-GGSGGS-nsp14 (ORF1ab Synthetic, SARS-CoV-2)

Tags

14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pIG-804_HA-GD2-28z_CAR_BATF-TFAP4

Plasmid

#207491

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

BATF-TFAP4, HA-GD2-28z_CAR (BATF Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS SARS-CoV-2 Delta Spike

Plasmid

#185593

Purpose

Expresses codon-optimized full length SARS-CoV-2 Delta Spike

Depositor

Marceline Côté

Article

Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

Insert

SARS-CoV-2 Delta Spike (S )

Expression

Mammalian

Mutation

Contains the following mutations: T19R, Δ156-157,…

Promoter

chicken β-actin promoter, CMV enhancer

Available Since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pIG-704_CD19-28z_CAR_tNGFR

Plasmid

#207486

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

tNGFR, CD19-28z_CAR (NGFR Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-702_CD19-BBz_CAR_tNGFR

Plasmid

#207485

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

tNGFR, CD19-BBz_CAR (NGFR Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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Spike Display_6P-D614G

Plasmid

#172734

Purpose

Expression vector for SARS-CoV-2 (HexaPro-D614G) spike used in the Spike Display system

Depositor

IIya Finkelstein

Article

Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

Insert

SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)

Tags

3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

Expression

Mammalian

Mutation

Ectodomain only (AAs 1-1208); 682-685 (furin site…

Promoter

CMV

Available Since

Aug. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pIG-727_HA-GD2-28z_CAR_RFP-tNGFR

Plasmid

#207487

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

RFP-tNGFR, HA-GD2-28z_CAR (NGFR Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-874_HA-GD2-28z_CAR_IL2RA

Plasmid

#207504

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

IL2RA, HA-GD2-28z_CAR (IL2RA Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-671_HA-GD2-28z_CAR_tNGFR

Plasmid

#207484

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

tNGFR, HA-GD2-28z_CAR (NGFR Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-TadCBEd-SpCas9-P2A-EGFP (BKS327)

Plasmid

#223123

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd-SpCas9 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadCBEd-SpCas9-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpCas9=D10A

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR207 SARS-CoV-2 NSP1 Delta RC

Plasmid

#164522

Purpose

Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions K164A/H165A)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Insert

NSP1 Delta RC (ORF1ab )

Expression

Mammalian

Mutation

Insert of NSP1 gene's CDS from SARS-CoV-2 is…

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS/XBB.1.16-RBD

Plasmid

#213070

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.16 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/XBB.1.16-RBD (S )

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLY57_TRAC-LHA-pAAV-EFS-CD22BBz-PRODH2-TRAC-RHA(3166mut)

Plasmid

#192188

Purpose

CD22 CAR AAV vector PRODH2 KI (pLY057)

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

CD22 CAR AAV vector PRODH2 KI (pLY057) (CD22 Synthetic)

Use

AAV; Mammalian expression

Mutation

NA

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pK27Sumo_His-SUMO-nsp14-nsp10 fusion (SARS-CoV-2)

Plasmid

#169160

Purpose

To express nsp14-nsp10 fusion protein in E. coli

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

14His-SUMO-nsp14-GGSGGS-nsp10 (ORF1ab Synthetic, SARS-CoV-2)

Tags

14His-SUMO and Fusion protein of SARS-CoV-2 nsp14…

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR207 SARS-CoV-2 NSP1 124A/125A

Plasmid

#164523

Purpose

Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions R124A/K125A)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Insert

NSP1 124A/125A (ORF1ab )

Expression

Mammalian

Mutation

Insert of NSP1 gene's CDS from SARS-CoV-2 is…

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)

Plasmid

#169164

Purpose

Baculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cells

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

nsp14/nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)

Tags

6His-3xFlag (nsp10 C-terminus)

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-10c)

Plasmid

#241036

Purpose

Expresses SARS-CoV-2 spike (B.1-10c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-10c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T76R,K113T,K147N,S155R,N439R,T478K,E484V,S494R,K5…

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-10b)

Plasmid

#241035

Purpose

Expresses SARS-CoV-2 spike (B.1-10b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-10b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K113T,K147N,S155R,Y248D,T478K,E484V,F490R,S494R,K…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-10d)

Plasmid

#241037

Purpose

Expresses SARS-CoV-2 spike (B.1-10d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-10d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

H69G,Y248D,R403T,N439R,T478K,E484V,F486E,F490R,S4…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-10a)

Plasmid

#241034

Purpose

Expresses SARS-CoV-2 spike (B.1-10a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-10a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

H69G,K113T,K147N,S155R,T478K,E484V,F490R,S494R,K5…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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