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pCMV-MBP-yATOM_mCherry

Plasmid

#209707

Purpose

Mammalian expression of y-ATOM biosensor for mCherry

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_mCherry

Tags

MBP

Expression

Mammalian

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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PSICORhp-mp21(v2)

Plasmid

#26272

Depositor

Rudolf Jaenisch

Article

Hanna et al Nature. 2009 Dec 3. 462(7273):595-601.

Insert

p21 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only
C122-E53: mClover3

Plasmid

#162898

Purpose

Standard Gateway entry clone encoding mClover fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Clover3 fluorescent protein

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMOD4 GFP

Plasmid

#19181

Depositor

Al Fisher

Article

Zhang et al BMC Dev Biol. 2008 Dec 30;8(1):119.

Insert

Green fluorescent protein

Tags

FLAG and daf-12 N-term 328 amino acids

Expression

Worm

Mutation

S65C, contains synthetic introns for C. elegans

Available Since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only

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PSICORhp-mp21(v1)

Plasmid

#26271

Depositor

Rudolf Jaenisch

Article

Hanna et al Nature. 2009 Dec 3. 462(7273):595-601.

Insert

p21 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-MBP-yATOM_Ras

Plasmid

#209709

Purpose

Mammalian expression of y-ATOM biosensor for HRas

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_Ras

Tags

MBP

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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ploxP unc-119

Plasmid

#19185

Depositor

Al Fisher

Article

Zhang et al BMC Dev Biol. 2008 Dec 30;8(1):119.

Insert

unc-119 (unc-119 Nematode)

Use

Recombineering

Mutation

contains loxP site just 5' to ApaI site

Available Since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

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C122-E16: CyPet

Plasmid

#162896

Purpose

Standard Gateway entry clone encoding CyPet fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

CyPet fluorescent protein

Use

Synthetic Biology

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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HuEV-A

Plasmid

#68342

Purpose

Gateway compatible human expression vector that adds a "flexible" 3XFLAG,V5,YFP N terminus tag

Depositor

Jef Boeke

Article

Mita et al Biol Proced Online. 2016 Aug 15;18:16. d

Type

Empty backbone

Use

Cre/Lox

Tags

3xFLAG, V5 and YFP

Expression

Mammalian

Promoter

TET-CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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C122-E17: mPlum

Plasmid

#162897

Purpose

Standard Gateway entry clone encoding mPlum fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Plum fluorescent protein

Use

Synthetic Biology

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pJOE4905.1

Plasmid

#135085

Purpose

rhamnose-inducible expression vector

Depositor

Josef Altenbuchner

Article

Motejadded et al Biotechnol Lett. 2009 Apr;31(4):543-9. d

Insert

P-rhaP-BAD-malE-His tag-smt3-eGFP

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Rab30

Plasmid

#31743

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab30 (Rab30 Fly)

Tags

GFP

Expression

Insect

Promoter

Actin

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pAS2-1-Esf1

Plasmid

#99987

Purpose

Express yeast Esf1 with Y2H binding domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Esf1 (ESF1 Budding Yeast)

Tags

Y2H binding domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pUC57-FASTKD4

Plasmid

#73620

Purpose

Cloning vector containing FASTKD4

Depositor

Vamsi Mootha

Article

Wolf et al Cell Rep. 2014 May 8;7(3):918-31. doi: 1

Insert

FASTKD4 (TBRG4 Human)

Mutation

FASTKD4 has been codon optimized for expression i…

Promoter

lac

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only
U371-GFP

Plasmid

#24476

Depositor

Ron Vale

Article

Tomishige et al Science. 2002 Sep 27. 297(5590):2263-7.

Insert

Unc104 (unc-104 Nematode)

Tags

GFP

Expression

Bacterial

Mutation

Contains only the kinesin's motor head fused…

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only
U371-HTR

Plasmid

#24469

Depositor

Ron Vale

Article

Tomishige et al Science. 2002 Sep 27. 297(5590):2263-7.

Insert

Unc104 (unc-104 Nematode)

Tags

His

Expression

Bacterial

Mutation

Contains only the kinesin's motor head domai…

Available Since

June 30, 2010

Availability

Academic Institutions and Nonprofits only
CJ24 (let-7 minigene)

Plasmid

#17764

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

dme-let-7 minigene (let-7 Fly)

Use

Drosophila metallothionein gene promoter drives e…

Expression

Insect

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
GFP-Rab23

Plasmid

#31742

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab23 (Rab23 Fly)

Tags

GFP

Expression

Insect

Promoter

Actin

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
U653-GFP ILtoEK

Plasmid

#24473

Depositor

Ron Vale

Article

Tomishige et al Science. 2002 Sep 27. 297(5590):2263-7.

Insert

Unc104 (unc-104 Nematode)

Tags

GFP

Expression

Bacterial

Mutation

I362E/L365K (neck destabilization mutant)

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-RBP-WDR5

Plasmid

#209702

Purpose

Mammalian expression of WDR5 with N terminal RBP fusion

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

RBP-WDR5

Tags

RBP

Expression

Mammalian

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Cox4-Clover

Plasmid

#209721

Purpose

Mammalian expression of clover localized to mitochondria

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

Cox4-Clover

Tags

Cos4

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_WDR5

Plasmid

#209706

Purpose

Mammalian expression of y-ATOM biosensor for WDR5

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_WDR5

Tags

MBP

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_SH2

Plasmid

#209708

Purpose

Mammalian expression of y-ATOM biosensor for SH2

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_SH2

Tags

MBP

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Cox4-SH2

Plasmid

#209717

Purpose

Mammalian expression of SH2 localized to mitochondria

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

Cox4-SH2

Tags

Cos4

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Cox4-NbGFP_miRFP670nano3

Plasmid

#209719

Purpose

Mammalian expression of NbGFP-miRFP670nano3 localized to mitochondria

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

Cox4-NbGFP_miRFP670nano3

Tags

Cos4

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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NODAL-matDYK

Plasmid

#115258

Purpose

For mammalian expression of the human NODAL open reading frame with an internal DYK tag

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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NODAL-T7TS

Plasmid

#115250

Purpose

For in vitro transcription of human NODAL open reading frame RNA

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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A0A0A1ZY49

Plasmid

#163186

Purpose

Inducible expression of UniProt identifier A0A0A1ZY49

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A0A1ZY49

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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A2CCV2

Plasmid

#163202

Purpose

Inducible expression of UniProt identifier A2CCV2

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A2CCV2

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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C122-E15: mOrange

Plasmid

#162895

Purpose

Standard Gateway entry clone encoding mOrange fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Orange fluorescent protein

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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