We narrowed to 18,730 results for: IRE
-
Plasmid#83238PurposeThe genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.DepositorInsertroGFP2-Tsa2dCr
UseTagsroGFP2 fusion with Tsa2dCrExpressionYeastMutationPromoterTEFAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO mTRPM7
Plasmid#45482DepositorInsertTRPM7 (Trpm7 Mouse)
UseTetracycline inducibleTagsFlagExpressionMammalianMutationPromoterTetracycline-controlled CMVAvailable sinceMay 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYCN_P2A_Hygro_Barcode
Plasmid#120463PurposeBarcoded lentiviral vector to express MYCN in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYCN (MYCN Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
8522-M01-663
Plasmid#225660PurposeLentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (green configuration)DepositorInsertdsCopGFP
UseLentiviralTagsExpressionMutationPromoterSORE6-mCMVpAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 K720A
Plasmid#19939DepositorInsertcullin 1 K720A (CUL1 Human)
UseTagsHA2ExpressionMammalianMutationCUL1 with a NEDD8 modification mutantPromoterAvailable sinceApril 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
VCP(DK0)-EGFP
Plasmid#23974DepositorInsertValosin containing protein (VCP Human)
UseTags6xHis, EGFP, and HAExpressionMammalianMutationE305QE578QPromoterAvailable sinceMarch 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMX-eSOX17FNV
Plasmid#118838PurposeRetroviral expression of a mutant of Sox17 that improves efficiency of iPSC generationDepositorInserteSOX17FNV (Sox17 Mouse)
UseRetroviralTagsExpressionMammalianMutationFNV: L46F, V53N, E57VPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-Hsp70-Cas9
Plasmid#46294PurposeA codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.DepositorInsertcodon optimized Cas9
UseCRISPRTags3x FlagExpressionInsectMutationSilent change from C to A at bp 834 of insertPromoterHsp70Available sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lenti-dual-CRISPR-U6-H1(pBP43)
Plasmid#218930PurposeThe backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhuman U6 and H1Available sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2
Plasmid#221712PurposeExpression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2 (ago2 Zebrafish)
UseFish expressionTags3xFLAGExpressionMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 Y42A/M43A
Plasmid#19940DepositorInsertcullin 1 Y42A/M43A (CUL1 Human)
UseTagsHA2ExpressionMammalianMutationCUL1 with a SKP1 binding mutantPromoterAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 delta 610-615
Plasmid#19941DepositorInsertcullin 1 delta 610-615 (CUL1 Human)
UseTagsHAExpressionMammalianMutationCUL1 with a ROC1 binding mutantPromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 delta C22
Plasmid#19942DepositorInsertcullin 1 delta C22 (CUL1 Human)
UseTagsHA2ExpressionMammalianMutationMutation of 22 amino acids from the C terminusPromoterAvailable sinceApril 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEBB Flag GCN5
Plasmid#74784Purposeexpress of GCN5 in mammalian cellsDepositorInsertGCN5 (KAT2A Human)
UseTagsFlagExpressionMammalianMutationPromoterEF-1alpha promoterAvailable sinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-H3.3-polyA
Plasmid#59780PurposeExpress Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertH3F3B (H3-3B Human)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
AKT-translocation-mStrawberry reporter
Plasmid#158685PurposeThis plasmid is a AKT pathway reporter. It has 1EF1a promoter driving the expression of truncated FoxO1 fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-CAG::Mitbow
Plasmid#158994PurposeMulticolor mitochondrial-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing mito-tethered mEYFP, mCherry or mCerulean upon Cre recombination)DepositorInsertMitbow
UseTagsThe default expressed EBFP2 is fused to H2B; oth…ExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-ATG8(416)/GFP-AUT7(416)
Plasmid#49425PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.DepositorInsertautophagy-related 8 (ATG8 Budding Yeast)
UseTagsGFPExpressionBacterial and YeastMutationPromoterATG8Available sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRG/III-hs-MBP-CB2-HF
Plasmid#121682PurposeBacterial expression of cannabinoid receptor CB2 fusion proteinDepositorInsertperipheral cannabinoid receptor (CNR2 Human)
UseTagsHis6 Flag tagExpressionBacterialMutationPromoterlacAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry
Plasmid#159655PurposeEmpty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Vamp2-pHluorin
Plasmid#190151PurposeExpresses (pH-sensitive) super ecliptic pHluorin-tagged mouse/rat Vamp2 for imaging exocytosisDepositorInsertVamp2 (Vamp2 Mouse)
UseAAVTagssuperecliptic pHluorinExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
EF1a_SOX10_P2A_Hygro_Barcode
Plasmid#120479PurposeBarcoded lentiviral vector to express SOX10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertSOX10 (SOX10 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
5xNF-kb_RE::RedF
Plasmid#124530PurposeNFKB RedFirefly luciferase reporterDepositorInsertNF-κβ RedF reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
413 pSG5L HA RB
Plasmid#10720DepositorInsertRB (RB1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
RAR-TRE-mStrawberry reporter
Plasmid#158683PurposeThis plasmid is a RAR pathway reporter. It has a Retinoic Acid Receptor Response Element promoter driving the expression of mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
p38-KTR-mStrawberry reporter
Plasmid#158687PurposeThis plasmid is a p38 pathway reporter. It has CMV promoter driving the expression of p38-KTR fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
ERK-KTR-mStrawberry reporter
Plasmid#158686PurposeThis plasmid is a ERK pathway reporter. It has CMV promoter driving the expression of ERK-KTR fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
AP-1 mu1-GFP
Plasmid#173432PurposeExpression of AP-1 Mu1 subunit with a terminal GFPDepositorInsertAP1M1 (Ap1m1 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW57-RFP-P2A-MCS (Neo)
Plasmid#89182PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.DepositorTypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
Mucolipin1-pHcRed C1
Plasmid#62959Purposefull length human Mucolipin-1 cloned into pHcRed1 C1DepositorInsertMucolipin-1 (MCOLN1 Human)
UseTagsHcRed1ExpressionMammalianMutationMucolipin-1 has 1 silent mutation compared to the…PromoterCMVAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP TMEM41B-GFP
Plasmid#117414PurposeExpresses TMEM41B tagged with GFP in mammalian cellsDepositorInsertTMEM41B (TMEM41B Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10/RSV_SF3B3-miniXon_Luciferase
Plasmid#174660PurposePlasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.DepositorInsertminiXon cassette
UseLuciferaseTagsExpressionMammalianMutationKozak ATG initiation sequence in middle exonPromoterRSVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nrf1-Myc pcDNA3.1(+)-C-Myc
Plasmid#169125Purposeexpression of proteinDepositorInserthNrf1 (NFE2L1 Human)
UseTagsExpressionMammalianMutationwtPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYCL_P2A_Hygro_Barcode
Plasmid#120462PurposeBarcoded lentiviral vector to express MYCL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYCL (MYCL Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_LIN28A
Plasmid#26819DepositorInsert5'UTR-LIN28A-3'UTR (LIN28A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
T7-Elongin C-pcDNA3
Plasmid#19998DepositorInsertElongin C (ELOC Human)
UseTagsT7-tagExpressionMammalianMutationPromoterAvailable sinceApril 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
drebrin-YFP
Plasmid#40359DepositorInsertdrebrin E2 (DBN1 Human)
UseChick expressionTagseYFPExpressionMutationPromoterchick B-actin promoter with CMV enhancerAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
PKA-KTR-mStrawberry reporter
Plasmid#158688PurposeThis plasmid is a PKA pathway reporter. It has CMV promoter driving the expression of PKA-KTR fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-EPLIN beta
Plasmid#40929DepositorInsertEPLIN beta (LIMA1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
JNK-KTR-mStrawberry reporter
Plasmid#158689PurposeThis plasmid is a JNK pathway reporter. It has CMV promoter driving the expression of JNK-KTR fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-ATP6V0D1
Plasmid#87931PurposeFLAG-tagged overexpressionDepositorInsertATP6Vod1 (ATP6V0D1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-HOIP-Strep-HA
Plasmid#113494PurposeExpression of human HOIP with C-terminal strep-HA tagDepositorInsertHOIP (RNF31 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAuroraA-GFP-AURKA
Plasmid#99877PurposeExpression of GFP-AURKA in mammalian cellsDepositorInsertAURKA (AURKA Human)
UseTagsGFPExpressionMammalianMutationPromoterAURKA minimalAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR
Plasmid#135500PurposeMammalian expression of FLAG-tagged TAPBPRDepositorInsertTAPBPR (TAPBPL Human)
UseTagsLuminal FLAG epitope tag and Signal peptide from …ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
LAMP1-SEP-mRuby3
Plasmid#200939Purposeendolysosome localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3DepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
C1-mCitrine-FKBP-EZRabd(t567D)
Plasmid#155227PurposeAcute increase in MPA when used with FRB-FKBP dimerizaton system upon addition with RapamycinDepositorInsertmCitrine fused to FKBP and the actin BD of Ezrin (T567D), aa552-586 for use with FRB and rapamycin induced dimerization system
UseTagsmCitrineExpressionMammalianMutationChanged Thr 567 to Asp in original EzrinPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
VCP(R155H)-EGFP
Plasmid#23972DepositorInsertValosin containing protein (VCP Human)
UseTags6xHis, EGFP, and HAExpressionMammalianMutationR155HPromoterAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
TRE reporter backbone
Plasmid#158678PurposeThis plasmid serves as the backbone for the TRE reporters described in our paper. It's a promoterless-mStrawberry-pGK-BSD backbone to which we insert a pathway specific promoter before the mStrawberryDepositorTypeEmpty backboneUseLentiviralTagsmStrawberryExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only