We narrowed to 13,411 results for: BASE

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pMSCVII-E2Crimson

Plasmid

#162756

Purpose

Retroviral (MSCV) expression of E2-Crimson fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

E2-Crimson

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCO602

Plasmid

#139735

Purpose

individual component of Fungal Bioluminescent Pathway, MoClo level 0 plasmid, NnCPH

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

CPH

Use

Luciferase; Moclo level 0 component

Promoter

none

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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AEB-F12

Plasmid

#63849

Purpose

This pFTV1 vector contains a fluoride binding riboswitch, controlling the translation initaition rate of mRFP1 gene.

Depositor

Howard Salis

Article

Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

Insert

F12 fluoride riboswitch

Use

Synthetic Biology

Promoter

J23100

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pGKO2

Plasmid

#63617

Purpose

a binary Agrobacterium vector carrying a modified HSVtk gene as a negative selection marker against ectopic transformants.

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Fungal expression

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-M2T1L

Plasmid

#33290

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

M2 (M2 Orthoreovirus T1L)

Expression

Mammalian

Promoter

T7

Available Since

Jan. 12, 2012

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mStayGold-Blast

Plasmid

#227254

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_LaG17-mCherry-HOTag3

Plasmid

#190024

Purpose

Transiently expresses GFP-binding CluMPS (LaG17-mCh-HOTag3) in mammalian cells and lentiviral vector for constitutive expression.

Depositor

Lukasz Bugaj

Article

Mumford et al Cell Syst. 2024 Feb 21;15(2):166-179.e7.

Insert

LaG17 CluMPS

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR_Cas9_sp1

Plasmid

#154851

Purpose

Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome

Depositor

John van der Oost

Article

Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.

Inserts

Spcas9 gene
sgRNA scaffold

Use

CRISPR

Tags

6xHis

Mutation

Codon harmonized variant for expression in Rhodob…

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-BFP

Plasmid

#106282

Purpose

A version of the pHAGE vector with BFP and dsRed expressed separated by an IRES. Note that dsRed is not expressed.

Depositor

Jay Shendure

Article

Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

Insert

CMV-BFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-43Q

Plasmid

#84352

Purpose

Expression of the human Huntingtin Exon1 protein containing 43Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon 1 (HTT Human)

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ121-mTCBE

Plasmid

#218172

Purpose

Express plastid monomeric TALE-linked CBE, composed of TALE, a catalytically impaired, full length DddA variant (E1347A), a human APOBEC3A variant (hA3A-Y130F), and UGI

Depositor

Yiping Qi

Article

Wang et al Plant Biotechnol J. 2024 May 6. doi: 10.

Insert

TALE-Intron-hA3A-Y130F-DddA full (E1347A)-UGI

Use

Plastid base editing

Expression

Plant

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2215_Nme2-rAPOBEC-i7

Plasmid

#201525

Purpose

Mammalian expression of domain inlaid Nme2-rAPOBEC-i7

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-rAPOBEC-i7

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RLuc8C-FHA1-NES

Plasmid

#138210

Purpose

Encodes PAABD fragment of luminescence-based bimolecular kinase activity reporters (LumKARs); cytosol targeted.

Depositor

Jin Zhang

Article

Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

Insert

RLuc8C-FHA1-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pJH124

Plasmid

#48259

Purpose

Template plasmid for PCR-based transplant of Ashbya gossypii TEF1 promoter in yeast.

Depositor

Ronald Davis

Article

Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

Inserts

URA3 3' fragment
URA3 5' fragment
Ag TEF1 promoter

Use

Routine cloning vector

Mutation

-242 upstream to URA3 ORF +495 and URA3 ORF from …

Promoter

Ag TEF1 promoter

Available Since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pJRK136

Plasmid

#173744

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::CIBN-3xFLAG::MP::unc-54 3' UTR

Expression

Worm

Promoter

Prps-0

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-ADARcd-YTHmut

Plasmid

#194431

Purpose

ADAR1 catalytic domain E488Q fused to YTHmut

Depositor

Kate Meyer

Article

Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

Insert

ADARcd fused to YTHmut domain (ADAR Human)

Tags

ADARcd-YTHmut-HA

Expression

Mammalian

Mutation

m6A binding region of YTH domain is deleted

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pJRK249

Plasmid

#173747

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::CRY2(olig)::VHH(mCherry)::unc-54 3' UTR

Expression

Worm

Promoter

Prps-0

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-TRP1-PGAL1-3HA

Plasmid

#41609

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

3xHA, TRP1 reverse orientation, and yeast ADH1 te…

Available Since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-PGAL1-GST

Plasmid

#41611

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-GST-TRP1

Plasmid

#41603

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

TRP1 (TRP1 Budding Yeast)

Use

Yeast genomic targeting

Tags

GST and yeast ADH1 terminator

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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