We narrowed to 15,090 results for: pET

1. YIp128-CUP1-His6-CUbo(K63R)-GFP

   Plasmid

   #212794

   Purpose

   Copper-inducible expression of the artificial test substrate His6-CUbo(K63R)-GFP in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   His6-CUbo(K63R)-GFP
   

   Use

   Integrative vector

   Tags

   Expression

   Yeast

   Mutation

   CUb(K63R)

   Promoter

   CUP1

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. YIp211-ADH-NUbo-E3(48) aa24-150-VSV

   Plasmid

   #212747

   Purpose

   Constitutive expression of NUbo-E3(48)ΔU7BD-VSV in budding yeast, inactive E3(48)

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(48) aa24-150
   

   Use

   Integrative vector

   Tags

   NUbo and VSV

   Expression

   Yeast

   Mutation

   Cue1 ΔU7BD

   Promoter

   ADH1

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. YIp211-ADH-myc-E3(1) C885A-CUbo-VSV

   Plasmid

   #212738

   Purpose

   Consitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1)

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(1)
   

   Use

   Integrative vector

   Tags

   CUbo-NLS-VSV and myc

   Expression

   Yeast

   Mutation

   HOIP(C885A)

   Promoter

   ADH1

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

   Plasmid

   #212805

   Purpose

   Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. H6GB1tevP53(304-393)

   Plasmid

   #200313

   Purpose

   bacterial expression of p53 TET-CTD

   Depositor

   Peter Wright

   Article

   Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

   Insert

   p53(304-393) (TP53 Human)
   

   Use

   Tags

   His6-GB1-tev

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. H6GB1tevP53(363-393)

   Plasmid

   #200314

   Purpose

   bacterial expression of P53 CTD

   Depositor

   Peter Wright

   Article

   Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

   Insert

   p53(363-393) (TP53 Human)
   

   Use

   Tags

   His6-GB1-tev

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pnEA-NpM-DmeIF4G_617-675-K633PQ638P-GB1_AD

   Plasmid

   #148510

   Purpose

   Bacterial Expression of DmeIF4G_617-675-K633PQ638P

   Depositor

   Elisa Izaurralde

   Article

   Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

   Insert

   DmeIF4G_617-675-K633PQ638P (eIF4G1 Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pnEA-NpM-DmeIF4Ga_617-630-DmThor_64-83-GB1_AD

   Plasmid

   #148513

   Purpose

   Bacterial Expression of DmeIF4Ga_617-630-DmThor_64-83

   Depositor

   Elisa Izaurralde

   Article

   Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

   Insert

   DmeIF4Ga_617-630-DmThor_64-83 (eIF4G1 Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pnEA-NpM-DmThor_50-83-Y54AM59A-GB1_AA

   Plasmid

   #148284

   Purpose

   Bacterial Expression of DmThor_50-83-Y54AM59A

   Depositor

   Elisa Izaurralde

   Article

   Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

   Insert

   DmThor_50-83-Y54AM59A (Thor Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pnEA-NvM-DmThor_Z

    Plasmid

    #148188

    Purpose

    Bacterial Expression of DmThor

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmThor (Thor Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pnEA-NvM-DmThor-CLL78AAA_Z

    Plasmid

    #148189

    Purpose

    Bacterial Expression of DmThor-CLL78AAA

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmThor-CLL78AAA (Thor Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pnEA-NvM-DmThor-GB1_Z

    Plasmid

    #148192

    Purpose

    Bacterial Expression of DmThor

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmThor (Thor Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pnEA-NvM-DmThor-Y54AM59A_Z

    Plasmid

    #148193

    Purpose

    Bacterial Expression of DmThor-Y54AM59A

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmThor-Y54AM59A (Thor Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pnEA-NvM-DmThor-YMCLLtoAAAAA_Z

    Plasmid

    #148194

    Purpose

    Bacterial Expression of DmThor-YMCLLtoAAAAA

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmThor-YMCLLtoAAAAA (Thor Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pnEA-NvG-DmThor_Z

    Plasmid

    #148184

    Purpose

    Bacterial Expression of DmThor

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmThor (Thor Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc5.1B-lambdaN-HA-DmThor-YMCLLtoAAAAA_Z

    Plasmid

    #148153

    Purpose

    Insect Expression of DmThor-YMCLLtoAAAAA

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmThor-YMCLLtoAAAAA (Thor Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc5.1B-EGFP-DmPAN2-F1103A-dsRNAres_Y

    Plasmid

    #148044

    Purpose

    Insect Expression of DmPAN2-F1103A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN2-F1103A-dsRNAres (PAN2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc5.1B-lambdaN-HA-DmPAN2-del130-133G-T97RH102G-dsRNAres_Y

    Plasmid

    #148064

    Purpose

    Insect Expression of DmPAN2-del130-133G-T97RH102G-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN2-del130-133G-T97RH102G-dsRNAres (PAN2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-lambdaN-HA-DmPAN2-del246-249-dsRNAres_Y

    Plasmid

    #148065

    Purpose

    Insect Expression of DmPAN2-del246-249-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN2-del246-249-dsRNAres (PAN2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAc5.1B-EGFP-DmPAN2-del130-133G-T97RH102G-dsRNAres_Y

    Plasmid

    #148033

    Purpose

    Insect Expression of DmPAN2-del130-133G-T97RH102G-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN2-del130-133G-T97RH102G-dsRNAres (PAN2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAc5.1B-EGFP-DmPAN2-del246-249-dsRNAres_Y

    Plasmid

    #148034

    Purpose

    Insect Expression of DmPAN2-del246-249-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN2-del246-249-dsRNAres (PAN2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAc5.1B-EGFP-DmPAN2-del358-467-T97RH102G-dsRNAres_Y

    Plasmid

    #148036

    Purpose

    Insect Expression of DmPAN2-del358-467-T97RH102G-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN2-del358-467-T97RH102G-dsRNAres (PAN2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAc5.1B-EGFP-DmCYFIP1_T

    Plasmid

    #147578

    Purpose

    Insect Expression of DmCYFIP1

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmCYFIP1 (Sra-1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    5 silent and one non silent A146V mutations compa…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAc5.1B-lambdaN-HA-DmCYFIP1_T

    Plasmid

    #147585

    Purpose

    Insect Expression of DmCYFIP1

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmCYFIP1 (Sra-1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    5 silent and one non silent A146V mutations compa…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAc5.1B-lambdaN-HA-DmCYFIP1-4xmut_T

    Plasmid

    #147586

    Purpose

    Insect Expression of DmCYFIP1-4xmut

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmCYFIP1-4xmut (Sra-1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    5 silent and one non silent A146V mutations compa…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAc5.1B-lambdaN-HA-DmCYFIP1-K743E_T

    Plasmid

    #147587

    Purpose

    Insect Expression of DmCYFIP1-K743E

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmCYFIP1-K743E (Sra-1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    5 silent and one non silent A146V mutations compa…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAc5.1B-lambdaN-HA-DmeIF4Ga_578-650-CG32016-E_T

    Plasmid

    #147588

    Purpose

    Insect Expression of DmeIF4Ga_578-650-CG32016-E

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmeIF4Ga_578-650-CG32016-E (eIF4G1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    CG32016-E 5 silent and three no silent mutations …

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAc5.1B-lambdaN-HA-DmThor-V5His6_G

    Plasmid

    #146398

    Purpose

    Insect Expression of DmThor

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmThor (Thor Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pnEA-NpM-HseIF4G_592-653_AG

    Plasmid

    #148746

    Purpose

    Bacterial Expression of HseIF4G_592-653

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    HseIF4G_592-653 (EIF4G1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pnYC-CvH-HseIF4E_24-217_AF

    Plasmid

    #148668

    Purpose

    Bacterial Expression of HseIF4E_24-217

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    HseIF4E_24-217 (EIF4E Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pT7-EGFP-C1-HseIF4G1_615-654_AD

    Plasmid

    #148518

    Purpose

    Mammalian Expression of HseIF4G1_615-654

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    HseIF4G1_615-654 (EIF4G1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. TTR C10S E63C

    Plasmid

    #190009

    Purpose

    Expression of human TTR in E coli

    Depositor

    Peter Wright

    Article

    Sun et al J Biol Chem. 2022 Jun 18;298(8):102162.

    Insert

    TTR (TTR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C10S, E63C

    Promoter

    T7

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. 2xNLS-NLP-cMyc AspCas12a

    Plasmid

    #182120

    Purpose

    pET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    2xNLS-NLP-cMyc AspCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. H2B-mNeonGreen_FRB(DmrC)_IRESpuro2

    Plasmid

    #182918

    Purpose

    Expesses H2B-mNeonGreen_FRB(DmrC). Can be used to target FKBP(DmrA) tagged proteins to H2B

    Depositor

    Daniel Gerlich

    Article

    Cuylen-Haering et al Nature. 2020 Nov;587(7833):285-290. doi:

    Insert

    pH2B (H2BC16P Human)
    

    Use

    Tags

    mNeonGreen-FRB(DmrC)

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV.U6gT28.4.hSyn.flex.H2B.RFP

    Plasmid

    #170386

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Cre-inducible expression of nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    Cre inducible H2B RFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV.U6gT28.3.hSyn.flex.H2B.RFP

    Plasmid

    #170374

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Cre-inducible expression of nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    Cre inducible H2B RFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV.U6gT28.13.hSyn.flex.H2B.RFP

    Plasmid

    #170376

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Cre-inducible expression of nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    Cre inducible H2B RFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLV-U6-UL29-U3-UL8

    Plasmid

    #166685

    Purpose

    To express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (non-EGFP version).

    Depositor

    Yujia Cai

    Article

    Yin et al Nat Biotechnol. 2021 Jan 11. pii: 10.103

    Insert

    UL8 and UL29 gRNA (UL29, UL8 HSV-1)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3HA-AMOT p130∆PPxY1(L106P)

    Plasmid

    #166714

    Purpose

    expresses AMOT(L106P) in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    Angiomotin p130 L106P (AMOT Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    changed leucine 106 to proline

    Promoter

    CMV promoter

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3HA-AMOT p130∆PPxY1(A112K)

    Plasmid

    #166715

    Purpose

    expresses AMOT(A112K) in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    Angiomotin p130 A112K (AMOT Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    changed alanine 112 to lysine

    Promoter

    CMV promoter

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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41. pDY153: Flag(SUZ12) WT HDR plasmid

    Plasmid

    #170793

    Purpose

    WT homology directed repair plasmid for performing genome-editing on the SUZ12 Locus

    Depositor

    Thomas Cech

    Article

    Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

    Insert

    SUZ12 (SUZ12 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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42. pRS313_ATP3G919C

    Plasmid

    #120255

    Purpose

    Expression of ATP3-7 in yeast

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

    Insert

    ATP3 (ATP3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    G919 changed to C; Alanine 307 changed to Proline

    Promoter

    ATP3

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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43. pRS313_ATP3mut

    Plasmid

    #120256

    Purpose

    Expression of ATP3-67 in yeast

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

    Insert

    ATP3 (ATP3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Isoleucine 304 changed to Asparagine; Alanine 307…

    Promoter

    ATP3

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. CMV-10xMS2 no wild type

    Plasmid

    #158204

    Purpose

    Cassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted form the oligo pool experiment. It does not contain the MS2-wt sequence, therefore QCP cannot bind it.

    Depositor

    Roee Amit

    Article

    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

    Insert

    10 binding sites of MS2 without the WT sequence from oligo library experiment
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. CMV-10xQb no wild type

    Plasmid

    #158205

    Purpose

    Cassette of 10 different binding sites with high affinity to Qb phage coat protein, as extracted form the oligo pool experiment. It does not contain the Qb-wt sequence, therefore MCP cannot bind it.

    Depositor

    Roee Amit

    Article

    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

    Insert

    10 binding sites of QB coat protein without the WT sequence based on the oligo library experiment
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pJT140_GalL_cCDA1-VRERBE3

    Plasmid

    #145103

    Purpose

    Expressing base editor cCDA1-VRERBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    cCDA1-VRERBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A/D1135V/G1218R/R1335E/T1337R)

    Promoter

    GalL

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. pJT122_GalL_cCDA1-VQRBE3

    Plasmid

    #145087

    Purpose

    Expressing base editor cCDA1-VQRBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    cCDA1-VQRBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A, D1135V, R1335Q, T1337R)

    Promoter

    GalL

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pJT113_GalL_cCDA1-xBE3

    Plasmid

    #145079

    Purpose

    Expressing base editor cCDA1-xBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    cCDA1-xBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

    Promoter

    GalL

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pRC/CMV-TYK2VF/PA-VSV

    Plasmid

    #139351

    Purpose

    Expression of the double mutant of TYK2-V678F-P1104A

    Depositor

    Sandra Pellegrini

    Article

    Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

    Insert

    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    V362F-V678F-P1104A

    Promoter

    CMV

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. pcDNA-HisA-HOXC6-2

    Plasmid

    #110365

    Purpose

    Mammalian expression of HisA-tagged HOXC6-2

    Depositor

    Carlos Moreno

    Article

    Ramachandran et al Oncogene. 2005 Jan 6;24(1):188-98. doi:

    Insert

    HOXC6 (HOXC6 Human)
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    short isoform

    Promoter

    Available Since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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