We narrowed to 25,321 results for: ina
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Plasmid#53767Purposemammalian expression of flag-tagged ATR N-terminal fragmentDepositorInsertATR (ATR Human)
TagsFlagExpressionMammalianMutationN-terminal domain, aa 1-1409PromoterCMVAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223-ACVR2B
Plasmid#23401DepositorAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-LATS2
Plasmid#23485DepositorAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pCDH-EF1-FHC-POLQ-DY2230AA
Plasmid#64878Purposelentiviral expression of human POLQ -DY2230AA mutant (a polymerase domain mutant)DepositorInsertPOLQ (POLQ Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationD2330A,Y2331A, in the DNA polymerase domain (POL)…PromoterEF1alphaAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEF-Bos-FLAG MDA5 MIII
Plasmid#52876PurposeExpresses MDA5 with a mutation to motif III in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIFIH1 (IFIH1 Human)
TagsFLAGExpressionMammalianMutationT488A S490AAvailable sinceMay 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
PKG I beta WT
Plasmid#16394DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPKG1 beta (PRKG1 Human)
TagsHAExpressionMammalianAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEBTet_NAD-Snifit-cyto
Plasmid#113914PurposePlasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNAD-Snifit
ExpressionMammalianPromoterCMV TetO2Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLIK DN RhoA
Plasmid#84646PurposeLentiviral expression of doxycycline-inducible dominant negative RhoA and co-expression of VenusDepositorAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.tgoGAP1
Plasmid#78737PurposeExpression of Ca2+ sensor GAP1 in the trans Golgi apparatusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttgoGAP1
TagsN terminal 81 amino acids of human galactosyltran…ExpressionMammalianMutationD117A/D119A/D163A in aequorin moietyPromoterCMVAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p413TEF-PKM1
Plasmid#34607DepositorAvailable sinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJFRC151-3XUAS-IVS-Flp2::PEST
Plasmid#32133DepositorPublicationInsertFlippase2::PEST
TagsPESTExpressionInsectMutationGlycine at Amino Acid 5Available sinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
sgRNA with U6 promoter
Plasmid#48962Purposeto drive the sgRNA expression under a U6 promoterDepositorPublicationTypeEmpty backboneUseCRISPRExpressionWormPromoterU6Available sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pM-CBE
Plasmid#196293Purpose35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GPDepositorInsertFull length TSWV M antigenome encoding CBE in place of viral GP
UseCRISPRTags3×flagExpressionPlantPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGH020_sgRNA_G418-GFP
Plasmid#85405Purposehu6 driven sgRNA vector with G418 and GFP selectable markersDepositorInsertG418 resistance and GFP
UseCRISPR and LentiviralExpressionMammalianAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LentiRCas9-CUG
Plasmid#104183PurposeLentiviral transfer vector that carries U6-driven sgRNA targeting CUG repeats using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)DepositorInsertU6-CUGsgRNA, EFS-PIN-dCas9
UseCRISPR and LentiviralAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDW464
Plasmid#8845DepositorTypeEmpty backboneTagsbiotin acceptor peptideExpressionInsectAvailable sinceNov. 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAT253
Plasmid#40943DepositorInsertGFP-LacI**
TagsGFP(S65T, V163A) and SV40 NLSExpressionYeastMutationLacI**/LacI2G(4 mutations) (see comment below); l…PromoterHis3 promoter fragmentAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-CMV-Flag-CDK12_IDG-K
Plasmid#135281PurposeGateway destination clone of CDK12 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-CDK12 (CDK12 Human)
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianPromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJEP317-pAAV-U6SaCas9gRNA(SapI)-EFS-GFP- KASH-pA
Plasmid#113694PurposeU6 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV and CRISPRTagsKASHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by