We narrowed to 569 results for: chat

1. pAAV-ITR-PytR(A2.1)-mCherry

   Plasmid

   #204869

   Purpose

   Expresses pyrrolysyl tRNA variant "A2.1" and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

   Insert

   M. mazei pyrrolysyl tRNA mutant "A2.1"
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   U25C; A3G, A4G, T65C, T66C, T67T

   Promoter

   U6

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-ITR-EcYtR(NGS-6)-mCherry

   Plasmid

   #217363

   Purpose

   Expresses E. coli tyrosine tRNA variant "NGS-6" and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

   Insert

   E. coli tyrosine tRNA mutant, "NGS-6"
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTET-IUPi

   Plasmid

   #122019

   Purpose

   Expresses IUP pathway genes (ChK, IPK, idi) under inducible promoter. aTC inducible. Kan. resist.

   Depositor

   Gregory Stephanopoulos

   Article

   Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

   Inserts

   Choline kinase (CKI1 Budding Yeast)
   Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
   isopentenyl-diphosphate Delta-isomerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E. coli

   Promoter

   pTET

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIDTSmart-CMV-PLRS1

   Plasmid

   #217365

   Purpose

   Expression of previously developed E. coli leucyl aminoacyl-tRNA synthesis variant that is "polyspecific"

   Depositor

   Abhishek Chatterjee

   Article

   Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2

   Insert

   leucyl-tRNA synthetase polyspecific mutant
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M40I, T252A, Y499I, Y527A, H529G

   Promoter

   CMV

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. PC1575

   Plasmid

   #232088

   Purpose

   Mammalian expression plasmid for ubiquibody (uAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and CHIPΔTPR CDS and a C-terminal IRES-mCherry cassette.

   Depositor

   Pranam Chatterjee

   Article

   Bhat et al Sci Adv. 2025 Jan 24;11(4):eadr8638. doi

   Insert

   uAb with cloning Esp3I restriction site
   

   Use

   Tags

   IRES-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAcBac1-EcTyr-TAG

   Plasmid

   #228782

   Purpose

   EcTyr aaRS/tRNA system for mammalian ncAA incorporation

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcTyrRS
   EcYtR
   BstR
   

   Use

   Baculoviral

   Tags

   Expression

   Mammalian

   Mutation

   TAG suppressor

   Promoter

   Available since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAcBac2.tR4-OMeYRS/GFP*

   Plasmid

   #50831

   Purpose

   baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells

   Depositor

   Peter Schultz

   Article

   Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

   Inserts

   two-copy Tyr tRNA cassette
   EGFP*
   OMeYRS
   two copy Tyr tRNA cassette
   

   Use

   Baculoviral

   Tags

   His, Myc-6xHis, and WPRE

   Expression

   Insect

   Mutation

   Y39TAG and able to charge various unnatural amino…

   Promoter

   CAG, CMV, and U6 and H1

   Available since

   June 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-ITR-PytR(PyOtR)-mCherry

   Plasmid

   #204870

   Purpose

   Expresses pyrrolysyl tRNA variant "PyOtR" and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

   Insert

   M. mazei pyrrolysyl tRNA mutant "PyOtR"
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   U25C; A3G, A4C, A5G, C6G, G63C, T65G, T66C

   Promoter

   U6

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-ITR-CMV-mCherry-1xU6-LtR-wt(TAG)

   Plasmid

   #217366

   Purpose

   Expresses E. coli leucine tRNA and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2

   Insert

   E. coli leucine tRNA for TAG suppression
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAcBac3-EcLeu TAG-EGFP**

    Plasmid

    #228785

    Purpose

    EcLeu aaRS/tRNA system for TAG suppression with EGFP containing two stop codons for dual suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcLeuRS
    EcLtR
    EGFP**
    

    Use

    Baculoviral

    Tags

    Expression

    Mammalian

    Mutation

    TAG suppressor

    Promoter

    Available since

    May 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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