We narrowed to 26,389 results for: pET

1. pET28a-gp32-H6

   Plasmid

   #163912

   Purpose

   Expresses T4 gp32 for bacterial expression and affinity purification

   Depositor

   Paul Freemont

   Article

   Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

   Insert

   gp32
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET30-2-GAPDH

   Plasmid

   #83910

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   glyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)
   

   Tags

   6x His tags

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETDuet-LahT150

   Plasmid

   #166785

   Purpose

   Cleavage of double glycine motif in peptides to remove the leader sequence

   Depositor

   Wilfred van der Donk

   Article

   Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

   Insert

   LahT150
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET23b-NTF2

   Plasmid

   #108918

   Purpose

   Expresses NTF2

   Depositor

   Larry Gerace

   Article

   Cassany et al Methods Mol Biol. 2009;464:181-205. doi:

   Insert

   NTF2
   

   Expression

   Bacterial

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-uvsX-H6

   Plasmid

   #163913

   Purpose

   Expresses uvsX for bacterial expression and affinity purification

   Depositor

   Paul Freemont

   Article

   Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

   Insert

   uvsX
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pet30b-5M SrtA

   Plasmid

   #51140

   Purpose

   expression plasmid for C-terminal his tagged S. aureus SrtA with enhanced catalytic activity

   Depositor

   Hidde Ploegh

   Article

   Shi et al Proc Natl Acad Sci U S A. 2014 Jul 15;11

   Insert

   S.aureus SrtA 5M
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   P94R, D160N, D165A, K190E, K196T

   Promoter

   T7

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-19b_MMLV-RT

   Plasmid

   #153312

   Purpose

   Bacterial expression of MMLV reverse transcriptase

   Depositor

   Philippa Marrack, Drew Endy

   Insert

   MMLV Reverse Transcriptase
   

   Tags

   10x His

   Expression

   Bacterial

   Available Since

   June 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. eSrtA(2A-9) pET29b

   Plasmid

   #75145

   Purpose

   reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity

   Depositor

   David Liu

   Article

   Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

   Insert

   reprogrammed sortase A variant
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   S102C A104H E105D K138P K152I N160K K162H T164N K…

   Promoter

   T7

   Available Since

   July 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-MHL

   Plasmid

   #26096

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Aug. 20, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pET22-HT/plsA

    Plasmid

    #73625

    Purpose

    Produces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tag

    Depositor

    James Williamson, T. J. Kappock

    Article

    Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

    Insert

    adenylate kinase (adk Escherichia coli str. K-12 substr. MG1655)
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEThT-RvLEAM

    Plasmid

    #90033

    Purpose

    To express His-tagged RvLEAM in bacteria

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

    Insert

    RvLEAM
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET31b-T7-Spinach2-Ct

    Plasmid

    #79158

    Purpose

    Expresses the Ct biosensor under a T7 promoter

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Insert

    T7-tRNA-Spinach2-Ct
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET31b-T7-Spinach2-Dp

    Plasmid

    #79159

    Purpose

    Expresses the Dp biosensor under a T7 promoter

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Insert

    T7-tRNA-Spinach2-Dp
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET23a-mUbc9

    Plasmid

    #53137

    Purpose

    E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    Ubc9 (Ube2i Mouse)
    

    Tags

    untagged

    Expression

    Bacterial

    Mutation

    protein sequence is identical to human

    Promoter

    T7

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET15b-RvLEAMshort

    Plasmid

    #218122

    Purpose

    Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

    Depositor

    Ci Ji Lim

    Article

    Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

    Insert

    RvLEAMshort
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Truncated form (58-181aa)

    Promoter

    T7

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET30-2-GPD1

    Plasmid

    #83911

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    glycerol-3-phosphate dehydrogenase 1 (GPD1 Human)
    

    Tags

    6x His tags

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-SnoopCatcher

    Plasmid

    #72322

    Purpose

    Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a-SnoopCatcher
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed G842T and D848G of S. pneumoniae adhesin …

    Promoter

    T7

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET24b-APEX2

    Plasmid

    #111702

    Purpose

    For IPTG-inducible expression of APEX2 in E. coli

    Depositor

    Jessica Seeliger

    Article

    Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

    Insert

    APEX2
    

    Tags

    6xHis and V5

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET_RTX-6xHis

    Plasmid

    #145029

    Purpose

    pET vector for IPTG-inducible, T7-based expression of RTX bearing a C-terminal 6xHis tag

    Depositor

    Andrew Ellington

    Article

    Bhadra et al BioProtocol 2021

    Insert

    RTX
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET_RTX_(exo-)

    Plasmid

    #102786

    Purpose

    Expresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)

    Depositor

    Andrew Ellington

    Article

    Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

    Insert

    reverse transcription xenopolymerase with 3'-5' deficient exonuclease
    

    Expression

    Bacterial

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET28 MBP_PrA/G-H6

    Plasmid

    #176077

    Purpose

    Express His6 tagged MBP_PrA/G in Escherichia coli

    Depositor

    Tom Rapoport

    Article

    Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    MBP PrA/G-H6
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. miniTurbo-His6_pET21a

    Plasmid

    #107178

    Purpose

    expresses His6-tagged miniTurbo in bacteria

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Expression

    Bacterial

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET24_NusA-Tus

    Plasmid

    #165959

    Purpose

    Expression of soluble E. coli Tus protein

    Depositor

    Keith Pardee

    Article

    Norouzi et al ACS Synth Biol. 2021 Jun 23. doi: 10.102

    Insert

    Tus
    

    Tags

    His-NusA-Tev

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET28a MDHC_YEAST

    Plasmid

    #204232

    Purpose

    Bacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDHC_YEAST (MDH2 Budding Yeast)
    

    Tags

    6X His

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28-mE1

    Plasmid

    #32534

    Depositor

    Jorge Eduardo Azevedo

    Article

    Carvalho et al Mol Biotechnol. 2011 Oct 20.

    Insert

    E1, ubiquitin-activating enzyme (Uba1 Mouse)
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET22b RNase A

    Plasmid

    #58903

    Purpose

    Bacterial expression of bovine pancreatic ribonuclease A

    Depositor

    Ronald Raines

    Article

    delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

    Insert

    RNase A (RNASE1 Bovine)
    

    Tags

    pelB sequence

    Expression

    Bacterial

    Mutation

    See Depositor Comments below

    Promoter

    T7

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a MDHM_YEAST

    Plasmid

    #204231

    Purpose

    Bacterial expression of yeast mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDHM_YEAST (MDH1 Budding Yeast)
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Does not include transit peptide and includes a M…

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET12a-PAPI

    Plasmid

    #19961

    Depositor

    Jonathan Tait

    Article

    Wood et al Blood. 1996 Sep 1. 88(5):1873-80.

    Insert

    Annexin V (ANXA5 Human)
    

    Expression

    Bacterial

    Available Since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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29. pET20b- hTPI

    Plasmid

    #50723

    Purpose

    overexpression of human TPI1 in E.coli

    Depositor

    Markus Ralser

    Article

    Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

    Insert

    Triosephosphate isomerase 1 (TPI1 Human)
    

    Tags

    6xHIS tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET30a-HS-Tn5

    Plasmid

    #127916

    Purpose

    Prokaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.

    Depositor

    Silin Zhong

    Article

    Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

    Insert

    Hyperstable Tn5
    

    Tags

    E coli elongation factor Ts and Mxe intein - Chit…

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    T7

    Available Since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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