We narrowed to 2,819 results for: PSI-P

1. pHES945

   Plasmid

   #112643

   Purpose

   blue light inducible cryptochrome based optogenetic expression system

   Depositor

   Hana El-Samad

   Article

   Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

   Insert

   CRY2PHR-GAL4DBD
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti-CaMKIIa-hChR2(C128S)-EYFP-WPRE

   Plasmid

   #20294

   Purpose

   Lentiviral expression of ChR2 (C128S) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

   Depositor

   Karl Deisseroth

   Article

   Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   changed Cysteine 128 to Serine

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-CaMKIIa-hChR2(C128A)-EYFP-WPRE

   Plasmid

   #20293

   Purpose

   Lentiviral expression of ChR2 (C128A) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

   Depositor

   Karl Deisseroth

   Article

   Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   changed Cysteine 128 to Alanine

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-CaMKIIa-hChR2(C128T)-EYFP-WPRE

   Plasmid

   #20295

   Purpose

   Lentiviral expression of ChR2 (C128T) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

   Depositor

   Karl Deisseroth

   Article

   Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   changed Cysteine 128 to Threonine

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. APCS-bio-His

   Plasmid

   #53424

   Purpose

   Expresses full-length Serum amyloid P-component precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   APCS (APCS Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pICH86966::AtU6p::sgRNA:NbCysP6

   Plasmid

   #223217

   Purpose

   Expresses an sgRNA targeting the NbCysP6 gene in Nicotiana benthamiana with an Arabidopsis U6 promoter

   Depositor

   Tsepo Tsekoa

   Article

   Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

   Insert

   NbCysP6 sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   None

   Expression

   Plant

   Mutation

   Promoter

   Arabidopsis U6 promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-CamKII-GFP-P2A-bReaches-minWPRE

   Plasmid

   #118276

   Purpose

   Expresses EGFP and bReaChES under a minimal CamKII Promoter

   Depositor

   Dietmar Schmitz

   Article

   Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

   Inserts

   EGFP
   bReaCHES
   

   Use

   AAV

   Tags

   P2A

   Expression

   Mutation

   Promoter

   CamK II

   Available since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-flex-oChIEF-citrine

   Plasmid

   #50973

   Purpose

   flexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITR

   Depositor

   Roger Tsien

   Article

   Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

   Insert

   ChIEF-citrine
   

   Use

   AAV

   Tags

   citrine

   Expression

   Mutation

   inverted and inserted between two lox sites

   Promoter

   human synapsin promoter

   Available since

   Feb. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-oChD-citrine

   Plasmid

   #50976

   Purpose

   AAV transfer vector with mammalian codon-optimized ChD-citrine between AAV2 ITR

   Depositor

   Roger Tsien

   Article

   Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

   Insert

   oChD-citrine
   

   Use

   AAV

   Tags

   citrine

   Expression

   Mutation

   ChD gene is mammalian codon optimized

   Promoter

   human synapsin promoter

   Available since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-At_PRORP1-His

    Plasmid

    #67869

    Purpose

    bacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tag

    Depositor

    Walter Rossmanith

    Article

    Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

    Insert

    PRORP1 (PRORP1 Mustard Weed)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Residues 36-572 (see comments)

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTS2333_Tier3(Lenti_inverse)-YPet_Puro

    Plasmid

    #169664

    Purpose

    Tier-3 vector for stable integration via lentiviral transduction in inverted direction with polyA carrying a constitutive expression cassette for puromyine and Ypet (PCMV-5'LTR-Psi-gag-env-PRPBSA-YPet-p2A-PuroR-pA::A2-pA::A3-pA-WPRE-3'LTR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven YPet and PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV / RPBSA

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPY22-mNeonGreen-Hyg

    Plasmid

    #137082

    Purpose

    Codon-optimized mNeonGreen for expression in Arabidopsis thaliana and Physcomitrella patens

    Depositor

    Gohta Goshima

    Article

    Yi et al Curr Biol. 2020 Jul 20;30(14):2860-2868.

    Insert

    mNeonGreen-Flag
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLJM1-NtermRGS2-GFP-CIBN

    Plasmid

    #112258

    Purpose

    Expresses alpha-helix component of opto-RGS2 in mammalian cells

    Depositor

    Brian Chow

    Article

    Hannanta-Anan et al ACS Synth Biol. 2018 Jun 15;7(6):1488-14

    Insert

    NtermRGS2-GFP-CIBN
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ChR2-2A-Halo

    Plasmid

    #22047

    Purpose

    Expression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activity

    Depositor

    Edward Boyden

    Article

    Han et al Front Mol Neurosci. 2009 Aug 27:2:12. do

    Insert

    ChR2-GFP-2A-Halo-YFP
    

    Use

    Tags

    GFP and YFP

    Expression

    Mammalian

    Mutation

    Note that there is a S147R mut in YFP. This mutat…

    Promoter

    CMV

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFH6

    Plasmid

    #86555

    Purpose

    Subcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiency

    Depositor

    Andreas Weber

    Article

    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

    Insert

    U6-26p::sgRNA scaffold
    

    Use

    CRISPR; Subcloning

    Tags

    Expression

    Mutation

    Promoter

    Arabidopsis U6-26 promoter

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SMR3B-bio-His

    Plasmid

    #52183

    Purpose

    Expresses full-length Submaxillary gland androgen-regulated protein 3B precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SMR3B (SMR3B Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUB-Cas9-@GL1

    Plasmid

    #86779

    Purpose

    Disruption of GLABROUS1 gene in Arabidopsis using CRISPR/Cas9

    Depositor

    Andreas Weber

    Article

    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

    Insert

    sgRNA against GLABROUS1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Arabidopsis U6-26 promoter

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAGM38869

    Plasmid

    #153220

    Purpose

    Level 0 Arabidopsis U6-promoter vector

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Insert

    Arabidopsis U6 snRNA promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RhGC(BE)_pGEM

    Plasmid

    #85469

    Purpose

    The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling

    Depositor

    Peter Hegemann

    Article

    Scheib et al Sci Signal. 2015 Aug 11;8(389):rs8. doi:

    Insert

    rhodopsin-guanylyl cyclase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hSyn-somBiPOLES-mCerulean

    Plasmid

    #154945

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge