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pED17x11

Plasmid

#134814

Purpose

sfGFP expression plasmid for characterizing qtRNA charging

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

sfGFP-151-TAGA

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

151-TAGA

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pED14xS-4

Plasmid

#134802

Purpose

IPTG inducible expression of S-TAGA-Evo3 qtRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

S-TAGA-Evo3 qtRNA

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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AlphaC

Plasmid

#124525

Purpose

pColE1_AlphaC

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial, Insect, and Mamm…

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pWS3867

Plasmid

#185744

Purpose

Sub-array backbone 3/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_mVenus

Plasmid

#118861

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

mVenus

Tags

His-tag

Expression

Bacterial

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pDmVasa

Plasmid

#165783

Purpose

Drosohphila vasa germline promoter and 5'UTR

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vasa promoter

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFC31

Plasmid

#165845

Purpose

_C31 integrase CDS

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

_C31 integrase

Expression

Insect

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pDmAlfaTub84b

Plasmid

#165781

Purpose

Drosophila Alpha Tubulin promoter

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

AlfaTub84b

Expression

Insect

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCRA03

Plasmid

#103141

Purpose

Triple gRNA expression separated with 28nt Csy4 motif

Depositor

Jens Nielsen

Article

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

gRNAs

Expression

Yeast

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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AlphaB

Plasmid

#124524

Purpose

pColE1_AlphaB

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial, Insect, and Mamm…

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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AlphaD

Plasmid

#124526

Purpose

pColE1_AlphaD

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial, Insect, and Mamm…

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pT7goldRNA

Plasmid

#47930

Purpose

In vitro transcription of golden gRNA

Depositor

Wenbiao Chen

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

golden gRNA (slc24a5 Zebrafish)

Use

CRISPR

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pED14xS-1

Plasmid

#134800

Purpose

IPTG inducible expression of S-TAGA qtRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

S-TAGA qtRNA

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pED14xS-WT

Plasmid

#134804

Purpose

IPTG inducible expression of WT S tRNA scaffold tRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

Wildtype S tRNA (decodes TCG)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pWS3868

Plasmid

#185745

Purpose

Sub-array backbone 4/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDmPolyUbiq

Plasmid

#165786

Purpose

Drosophila Polyubiquitin promoter, strongest promoter in the library

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

PolyUbiq

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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phlFd-mLin

Plasmid

#140793

Purpose

CMV-phlFo2-phlFR-2A-mCherry mammalian synthetic gene circuit

Depositor

Gabor Balazsi

Article

Szenk et al ACS Synth Biol. 2020 Apr 17;9(4):930-939

Insert

phlFd-mLinearizer

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pED14xS-11

Plasmid

#134803

Purpose

IPTG inducible expression of S-TAGA-Evo1 qtRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

Wildtype S tRNA (decodes TCG)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pUS

Plasmid

#153416

Purpose

Backbone for cloning downstream of UCOE-SFFV

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pED14xY-3

Plasmid

#134808

Purpose

IPTG inducible expression of Y-TAGA-Evo1 qtRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

Y-TAGA-Evo1 qtRNA

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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