We narrowed to 42,035 results for: Vit;
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Plasmid#74142PurposeMammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicatorDepositorInsertFlicR1
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
p189
Plasmid#65207PurposeMutant EGFP expression plasmid for the creation of a heteroduplex DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see notes in comments section.DepositorInsertEGFP
UseTagsExpressionBacterial and MammalianMutationPremature stop codon added to pGEM5Z(+)-EGFP (TGG…PromoterCMVAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
Talin-CS
Plasmid#83377PurposeControl FRET probe for measuring tension on Talin moleculeDepositorInsertTalin1 (Tln1 Mouse)
UseRetroviralTagsEGFP-F40-tagRFP (tension sensor module)ExpressionMammalianMutationTension sensor module is inserted at C-terminal o…PromoterCMVAvailable sinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)
Plasmid#149722PurposeLentiviral expression of crRNA with GFP and a neomycin resistance geneDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for crRNA expression and EFS promoter…Available sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCuo DN Rac1
Plasmid#84644Purposedominant negative Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.DepositorInsertRac1 (RAC1 Human)
UseLentiviralTagsExpressionMammalianMutationDominant NegativePromotercumate-inducible promoterAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
p206-Switch-ON
Plasmid#217885PurposeRetroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoRDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and RetroviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
p205-5'-Switch-ON
Plasmid#217884PurposeRetroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoRDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and RetroviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E-mpeg1.1
Plasmid#75023PurposeCan be used to drive expression in zebrafish microglia.DepositorInsertmacrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)
Use5' entry vector for multisite gateway three …TagsExpressionMutationPromotermpeg1.1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
VTN-bio-His
Plasmid#53429PurposeExpresses full-length Vitronectin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertVTN (VTN Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDARMO_HUWE1_3xFLAG
Plasmid#187155Purposemammalian cell expression of HUWE1-3xFLAGDepositorInsertHUWE1 (HUWE1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RAM-d2TTA::TRE-FLEX-MCS-WPRE-pA
Plasmid#84467PurposeEmpty pAAV-CRAM plasmid w. multiple cloning site (MCS)DepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterpRAM and TREAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_FL_WT
Plasmid#98651PurposeExpresses MBP-tagged full length FUSDepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadCBEd
Plasmid#193835PurposeExpress TadCBEd (with SpCas9) in mammalian cellsDepositorInsertTadA-CDd-SpCas9 D10A-2xUGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sniper2L
Plasmid#193856PurposeExpresses human codon-optimized Sniper2L and blasticidin resistance: EFS promoter-Sniper2L-NLS-FLAG-P2A-BSDDepositorInsertSniper2L
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationE1007LPromoterEFSAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBR1.cas9-NT
Plasmid#197871PurposeConstitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)DepositorInsertstrancCRNA
Cas9
crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i1 ONE-GO
Plasmid#189735PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1
Plasmid#187120Purposemammalian cell expression of FLAG-HUWE1DepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.GCaMP6s.WPRE.SV40
Plasmid#100843PurposeAAV expression of ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6s
UseAAVTagsExpressionMammalianMutationGCaMP3-K78H T302L R303P D380Y T381R S383T R392GPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSG5-HA-ERR3 S45A
Plasmid#37849DepositorInsertEstrogen-related receptor gamma (Esrrg Mouse)
UseTagsHAExpressionMammalianMutationchanged Serine 45 to Alanine (AGC to GCC)PromoterSV40Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
Watermelon backbone
Plasmid#155258PurposeCan be used to generate a Watermelon libraryDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-Hyg-Dest-mCherry-PAR-6B
Plasmid#194908PurposeTo generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.DepositorInsertPAR-6B (Pard6b Mouse)
UseMouse TargetingTagsmCherryExpressionMammalianMutation39 C to G, silent mutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEXtev-HMG54kd
Plasmid#26030DepositorInsert3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR Human)
UseTagsGSTExpressionBacterialMutationCatalytic domain (amino acids 426-488) Methion…PromoterAvailable sinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28-His-TEV-NQO1
Plasmid#119058PurposeFor expression in E.coli and affinity purification of N-terminally His tagged NQO1DepositorInsertNAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
UseTags6His, TEV siteExpressionBacterialMutationPromoterT7Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-MRE11-WT
Plasmid#82033PurposePlasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInsertMRE11 (MRE11 Human)
UseTagsHAExpressionMammalianMutationPromoterCMV-tetAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-q ONE-GO
Plasmid#189738PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-JNK1a1
Plasmid#47574PurposeBacterial expression plasmid for GST-3xFlag-tagged JNK1a1DepositorInsertJNK1a1 (MAPK8 Human)
UseTags3xFlag and GSTExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEF1α:flex-mcherry
Plasmid#222618PurposeCre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoterDepositorInsertCre-dependent mCherry
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.GCaMP6s.WPRE.SV40
Plasmid#100842PurposeAAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6s
UseAAVTagsExpressionMammalianMutationGCaMP3-K78H T302L R303P D380Y T381R S383T R392GPromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGKCMV)-nucTomato-hygro
Plasmid#208573PurposeLV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an PGK/CMV promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmnucTomato
d2nucEGFP
HygroR
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterPGK/CMV and minPAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Puro_siKD
Plasmid#86695PurposeTetracycline inducible expression of shRNA targeted to AAVS1 locusDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterH1 TOAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
OPRM1-DuET
Plasmid#213361PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertOPRM1 (OPRM1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CAG-42B3mutAC2(Ser2ph)-SNAP
Plasmid#174876PurposePiggyBac transposon vector for expression of RNAPII Ser2ph mintbodyDepositorInsertRNAPII Ser2ph mintbody-SNAP
UsePiggybac vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-GSDMD-Myc
Plasmid#201166PurposeExpression of GSDMD gene in mammalian cells by retroviral transductionDepositorInsertGasdermin D (Gsdmd Mouse)
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Hygro DEST ERKKTRmCerulean3
Plasmid#90229PurposeLentiviral vector to express ERK KTR mCerulean3 under PGK promoter (With Hygromycin Resistance)DepositorInsertERK Kinase Translocation Reporter
UseLentiviralTagsmCerulean3ExpressionMammalianMutationPromoterPGKAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadDE
Plasmid#193837PurposeExpress TadDE (with SpCas9) in mammalian cellsDepositorInsertTadA-DualEditor-Cas9D10A-2xUGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.GCaMP6f.WPRE.SV40
Plasmid#100837PurposeAAV expression of ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6f
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMART Tmem192-3X HA (targeting vector for genomic tagging)
Plasmid#175777PurposepSMART Tmem192-3X HA (targeting vector for genomic tagging)DepositorInsertTMEM192 (TMEM192 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a-C2-mKate
Plasmid#208763PurposeTo express C2-mKate in bacteriaDepositorInsertC2-mKate (Mfge8 Mouse)
UseTagsmKateExpressionBacterialMutationPromoterT7 promoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_DA2m
Plasmid#140553PurposeExpresses the 2nd generation medium-affinity DA sensor GRAB_DA2m in neuronsDepositorHas ServiceAAV9InsertThe 2nd generation DA sensor GRAB_DA2m (medium affinity)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
AnkyrinG-190-GFP
Plasmid#31059DepositorInsertAnkyrin G (Ank3 Rat)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2012AvailabilityAcademic Institutions and Nonprofits only