We narrowed to 14,617 results for: Cre
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Plasmid#122508PurposeHomology repair template for in frame (first exon) clover knock-in of human LMNA geneDepositorInsertHomology Repair Template for Human LMNA (N-Terminal Clover Tag) (LMNA Human)
UseHomology repair template plasmid (donor plasmid)TagsCloverAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDH_Str-KDEL_ST-SBP-EGFP
Plasmid#65267Purposesynchronize trafficking of ST from the ER (RUSH system)DepositorInsertStr-KDEL and truncated sialyltransferase (ST6GAL1 Human)
UseLentiviralTagsEGFP and Streptavidin Binding Protein (SBP)Mutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+CMV-Ins-GLuc
Plasmid#89929PurposeExpresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman insulin-Gaussia-Luciferase (INS Synthetic, Human)
ExpressionMammalianMutationDNA sequence for Gaussia luciferase was humanized…PromoterCMVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3 RARE-d2EGFP
Plasmid#183055PurposeDestabilized EGFP fluorescent reporter for Retinoic Acid activityDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertd2EGFP
PromoterRARE, SV40Available sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-16
Plasmid#172746PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-16
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCold TDP43 Q331K
Plasmid#27454DepositorInsertTDP-43 (TARDBP Human)
TagsFactor Xa and His6ExpressionBacterialMutationGlutamine 331 mutated to Lysine (Q331K)Available sinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
CMV-H2B-mScarlet-I
Plasmid#182297PurposeLocalization of mScarlet-I fluorescent protein to histone 2BDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmScarlet-I
TagsmScarlet-IExpressionMammalianAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-Wnt11
Plasmid#35922DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertWnt11 (wnt11f2 Human)
ExpressionMammalianMutationNonePromoterCMVAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR
Plasmid#172982Purposecontrol & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusionDepositorInsertcontrol sgRNA
UseCRISPR and LentiviralAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1neo-14-3-3-CLuc
Plasmid#107611Purposemeasure LATS activity by Luciferase AssayDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpCDNA3.1hygro-BamHI-CLuc1433-NotI
ExpressionMammalianAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRS416 Gal M337V YFP
Plasmid#27449DepositorInsertTDP-43 (TARDBP Human)
TagsYFPExpressionYeastMutationMethionine 337 mutated to Valine (M337V)Available sinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMX Vav1 Y3F uGFP
Plasmid#14558DepositorInsertVav1 (VAV1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationY3F (Y142, Y160, Y174) in DH domainAvailable sinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLT 653
Plasmid#59996PurposeExpresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/klp-16 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progenyDepositorExpressionBacterialMutationpartial genomicAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-GST-eIF4E K119A
Plasmid#112818PurposeExpresses N-terminally GST-tagged mouse Eif4e K119A in bacterial cellsDepositorInsertEif4e (Eif4e Mouse)
TagsGSTExpressionBacterialMutationK119 mutated to A, increasing affinity for cap st…PromoterT7Available sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDH_TNF-SBP-EGFP
Plasmid#65282PurposeTNF reporter only (no hook) (RUSH system)DepositorAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hHNF4alpha
Plasmid#206878PurposeThe ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the Bam HI/XbaI sites of the pcDNA3.1(+), the mammalian expression vector.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHuman hepatocyte nuclear factor 4 alpha (HNF4A Human)
ExpressionMammalianPromoterCMVAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-P3-Alb-mScarlet
Plasmid#183461PurposeTo produce AAV with the P3 promoter to express Alb(mouse)-mScarlet fusion proteinDepositorAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Ii-Str_neomycin
Plasmid#65308PurposeLuminal ER hook only (RUSH system)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertInvariant chain (p33)
ExpressionMammalianMutationStreptavidin in its lumenal partPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TDP-REGv2(mScarlet-FLAG)#9 in pTwist-CMV backbone
Plasmid#216153PurposeExpresses mScarlet in cells with TDP-43 loss-of-function. Has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells (brighter than #7). It uses a single intron. [Code 'B11']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor CommentsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmScarlet with cryptic splice site and C-terminal FLAG tag
ExpressionMammalianAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE55
Plasmid#90400PurposeC-REL - NFkB gene reporterDepositorInsertFirefly luciferase
UseLentiviralPromoterCRELAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only