We narrowed to 31,228 results for: PLE;

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iRFP-FRB-Rab5

Plasmid

#51612

Purpose

Recruiter to Rab5-positive endosomes

Depositor

Tamas Balla

Article

Hammond et al J Cell Biol. 2014 Apr 7.

Inserts

FRB
Rab5

Tags

iRFP

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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isce rfp/cfp

Plasmid

#83323

Purpose

fluorescent Isce joint substrate

Depositor

Katheryn Meek

Article

Neal et al J Immunol. 2016 Apr 1;196(7):3032-42. do

Insert

RFP +CFP joining substrate

Use

Synthetic Biology

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pTrig-CopA

Plasmid

#104578

Purpose

Copy number inducible plasmid. Contains mini-F origin for maintenance and P1 lytic replication machinery under CopA promoter for copy increase.

Depositor

Harris Wang

Article

Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

Insert

PCopA-RiboJ-B0034-repL

Use

Synthetic Biology

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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290 RFP/CFP

Plasmid

#83322

Purpose

fluorescent coding joint substrate

Depositor

Katheryn Meek

Article

Neal et al J Immunol. 2016 Apr 1;196(7):3032-42. do

Insert

RFP +CFP joining substrate

Use

Synthetic Biology

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pGC4593

Plasmid

#110206

Purpose

Accessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)

Depositor

Adam Arkin, Guillaume Cambray

Article

Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

Inserts

TEV protease
aaRS

Expression

Bacterial

Promoter

Pbad and Ptet

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pSA101

Plasmid

#59787

Purpose

drives ZIF-1 protein in C. elegans neurons

Depositor

Jeremy Nance

Article

Armenti et al Development. 2014 Dec;141(23):4640-7. do

Inserts

rab-3 promoter
zif-1 coding (zif-1 Nematode)
unc-54 3' UTR

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pJexpress414_OmpA171_WT

Plasmid

#62646

Purpose

Wild-type OmpA (N-terminal domain) with an HA tag in the 4th external loop

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Promoter

T7

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCRA03

Plasmid

#103141

Purpose

Triple gRNA expression separated with 28nt Csy4 motif

Depositor

Jens Nielsen

Article

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

gRNAs

Expression

Yeast

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMZ288

Plasmid

#59897

Purpose

Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+

Depositor

Mikel Zaratiegui

Article

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Inserts

Cas9
rrk1:sgRNA

Use

CRISPR

Expression

Yeast

Mutation

Silent muation of the CspCI site

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pRS426-GFP-PLCdelta-PH

Plasmid

#71375

Purpose

For analysis of PH domain localization in yeast

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

PLCdelta-PH

Tags

EGFP

Expression

Yeast

Mutation

Pleckstrin homology (PH) domain

Available Since

Nov. 2, 2016

Availability

Academic Institutions and Nonprofits only
pAWp9

Plasmid

#73851

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-{BamHI+EcoRI}

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

UBCp-RFP-CMVp-GFP-{BamHI+EcoRI}

Use

Lentiviral

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pAM3558

Plasmid

#40252

Depositor

Susan Golden

Article

Chen et al Plasmid. 2008 May;59(3):176-92. Epub 200

Insert

Gm cassette (BssHII+SphI)

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pAW204

Plasmid

#113244

Purpose

E. coli expression vector for IHFa F13A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa F13A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

F13A

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMZ289

Plasmid

#59898

Purpose

Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-M210

Depositor

Mikel Zaratiegui

Article

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Inserts

Cas9
rrk1:sgRNA

Use

CRISPR

Expression

Yeast

Mutation

Silent muation of the CspCI site

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pMulti-sgRNA-LacZ

Plasmid

#99913

Purpose

Receptor plasmid for the assembly of multiple sgRNAs

Depositor

Yujie Sun

Article

Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAW206

Plasmid

#113246

Purpose

E. coli expression vector for IHFa K15A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa K15A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

K15A

Promoter

T7

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAW212

Plasmid

#113234

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->CCATC

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->CCATC

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMZ381

Plasmid

#59899

Purpose

Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-L469

Depositor

Mikel Zaratiegui

Article

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Inserts

Cas9
rrk1:sgRNA

Use

CRISPR

Expression

Yeast

Mutation

Silent muation of the CspCI site

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pJJH1242

Plasmid

#36909

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Insert

ScURA3

Use

Lentiviral

Expression

Bacterial, Mammalian, and Y…

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only

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pT7-stl

Plasmid

#51721

Purpose

conditional gene knockdowns in Trypanosoma brucei

Depositor

Arthur Gunzl

Article

Brandenburg et al EMBO J. 2007 Nov 28;26(23):4856-66. Epub

Type

Empty backbone

Use

Conditional gene knockdown in t. brucei

Promoter

T7 (2X)

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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