We narrowed to 7,695 results for: ALP

1. pGM79

   Plasmid

   #220171

   Purpose

   CRISRPi guide RNA against MARCHF5

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   MARCHF5 (sgRNA) (MARCHF5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRetroX-TRE3G_FLAG-CRTC1/MAML2_PGK-GpNLuc

   Plasmid

   #154265

   Purpose

   Dox/Tet-inducible expression of the CRTC1-MAML2 fusion oncogene in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

   Insert

   FLAG-CRTC1/MAML2 fusion oncogene
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-DIO-P-Ef1a-mCherry-T2A-RPL22-1xV5

   Plasmid

   #170328

   Purpose

   Double-floxed inverse Orientation V5 and mCherry tagged RPL22

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-CDC27 wt

   Plasmid

   #31673

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   cell division cycle 27 homolog (CDC27 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

5. QSOX1-bio-His

   Plasmid

   #51825

   Purpose

   Expresses full-length Sulfhydryl oxidase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   QSOX1 (QSOX1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

   Plasmid

   #141236

   Purpose

   AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)

   Depositor

   Guohong Cui

   Article

   Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

   Insert

   NES-jRGECO1a
   

   Use

   AAV and Cre/Lox; Cre-off

   Tags

   6xHIS tag and nuclear export signal

   Expression

   Mutation

   Promoter

   human elongation factor-1 alpha (EF-1 alpha)

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOTTC446 - pAAV EF1a hPREP(S554A)

   Plasmid

   #59968

   Purpose

   An AAV packaging vector that expresses catalytically-inactive hPREP under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

   Insert

   Prolyl oligopeptidase (PREP Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   S554A

   Promoter

   EF1a

   Available Since

   Oct. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EF1_Cre

   Plasmid

   #201198

   Purpose

   AAV vector for cre expression under EF1 promoter

   Depositor

   Tetsuo Yamamori

   Article

   Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

   Inserts

   cre
   EF1alpha promoter
   

   Use

   AAV

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   nuclear targeting cre

   Promoter

   N/A and hEF1alpha promoter

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-EF1a-hLMX1A-T2A-copGFP

   Plasmid

   #170444

   Purpose

   Lentiviral vector expressing human LMX1A (with silent mutation) with copGFP reporter gene, under control of EF1alpha promoter.

   Depositor

   Sang-Hun Lee

   Article

   Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

   Insert

   human LMX1A
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CD226-bio-His

    Plasmid

    #51646

    Purpose

    Expresses full-length CD226 antigen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    CD226 (CD226 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF2597

    Plasmid

    #142963

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RORA (RORA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. CD8-pTagRFP657-LOVpep (T406A, T407A, I532A)

    Plasmid

    #100742

    Purpose

    To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    CD8A (CD8A Human)
    

    Use

    Tags

    LOVpep and tagRFP657

    Expression

    Mammalian

    Mutation

    Truncated

    Promoter

    CMV

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Human b4 nicotinic receptor subunit EM construct

    Plasmid

    #163961

    Purpose

    pEZT-BM vector with beta4 gene after CMV promoter, published EM construct

    Depositor

    Ryan Hibbs

    Article

    Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do

    Insert

    Human beta4 nicotinic acetylcholine receptor subunit EM construct
    

    Use

    Baculovirus (bacmam) production and mammalian exp…

    Tags

    StrepII tag and apocytochrome b(562)RIL (BRIL)

    Expression

    Mammalian

    Mutation

    Pro341-Ser395 replaced with apocytochrome b(562)R…

    Promoter

    CMV

    Available Since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF0499

    Plasmid

    #143881

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PPARGC1A (PPARGC1A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. ECE1-His-bio

    Plasmid

    #53361

    Purpose

    Expresses full-length Endothelin converting enzyme 1 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    ECE1 (ECE1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEM-HSPA5P

    Plasmid

    #44150

    Purpose

    a fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)

    Depositor

    Joan Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    HSPA5 promoter (HSPA5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pLV-shEsr2

    Plasmid

    #120722

    Purpose

    Lentiviral vector that expresses GFP and an shRNA targeting Esr2 (in pLL3.7).

    Depositor

    Amy Lasek

    Article

    Vandegrift et al J Neurosci. 2020 Jul 1;40(27):5196-5207.

    Insert

    Esr2 (Esr2 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRC/CMV-TYK2K930R-VSV

    Plasmid

    #139346

    Purpose

    Expression of kinase-dead TYK2-K930R protein

    Depositor

    Sandra Pellegrini

    Article

    Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

    Insert

    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    V362F-K930R

    Promoter

    CMV

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBabe puro-HA-PIK3CA(H1047R;F486S;del)

    Plasmid

    #33255

    Depositor

    Thomas Roberts

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    Hemagglutinin (HA)

    Expression

    Mammalian

    Mutation

    changed histidine 1047 to arginine and phenylalan…

    Promoter

    SV40

    Available Since

    Jan. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pCR3-FLAG-Gephyrin GC

    Plasmid

    #68818

    Purpose

    Flag tagged mammalian expression of GC domain

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    NIL

    Promoter

    CMV

    Available Since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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