We narrowed to 5,563 results for: cycs

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pFP.R267

Plasmid

#138179

Purpose

Expresses the split mCherry reporter interrupted by the gp41-1 intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(SGY).gp41-1.(SSS)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pIE2-DBD

Plasmid

#21602

Depositor

Takahiro Kusakabe

Article

Mon et al Anal Biochem. 2009 Sep 15;392(2):180-2.

Insert

GAL4 DBD (GAL4 Budding Yeast)

Use

Gateway destination vector

Tags

3xHA and Gateway destination cassette

Expression

Insect

Available Since

March 9, 2010

Availability

Academic Institutions and Nonprofits only

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pCDM8 hCD81

Plasmid

#11588

Depositor

Shoshana Levy

Article

Oren et al Mol Cell Biol. 1990 Aug . 10(8):4007-15.

Insert

CD81 (CD81 Human)

Expression

Mammalian

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
pJM251 (miniCTX1-araC-ParaBAD)

Plasmid

#110567

Purpose

Integration vector for Pseudomonas aeruginosa with araC-ParaBAD inducible promoter

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

araC-ParaBAD

Expression

Bacterial

Promoter

araC-ParaBAD

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pFP.R377

Plasmid

#138247

Purpose

Expresses the split mCherry reporter interrupted by the Mja-KlbA intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(HDG).Mja-KlbA*.(CSG)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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Hygro-iDEST

Plasmid

#75339

Purpose

GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

TRE-DEST

Expression

Mammalian

Promoter

TRE-tight

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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pTS93 pHAGE2 CMVtetO2 GFP-22xGS-MCS

Plasmid

#199346

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-YFP-SLBP(18-126)-IRES-H1-Skylan62A

Plasmid

#205760

Purpose

Single-color FUCCI4 sensor for S/G2 cell cycle transition and G2/M transition

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

YFP-SLBP(18-126)-IRES-H1-Skylan62A

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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LT2_12_GlyRNA

Plasmid

#177710

Purpose

Plasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pEB2_mScarletI_mVenusNB-MrkH

Plasmid

#182291

Purpose

expresses c-di-GMP biosensor in E. coli

Depositor

Howard Berg

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2022 Feb 8;119

Insert

mVenusNB-MrkH

Tags

mVenusNB internal fusion to MrkH

Expression

Bacterial

Promoter

proC

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pRG706_lexO-SbfI_LexA-TF_LEU2MX

Plasmid

#154818

Purpose

Inducible expression of SbfI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

SbfI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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XLone-Cas9-2A-GFP

Plasmid

#170159

Purpose

Express Cas9 and GFP upon doxycycline treatment

Depositor

Xiaojun Lian

Article

Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

Insert

Cas9

Use

Synthetic Biology; Piggybac

Tags

GFP

Expression

Mammalian

Promoter

TRE3G promoter

Available Since

July 14, 2024

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-NLS-PTEN

Plasmid

#206984

Purpose

Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with NLS

Depositor

Ming Chen

Article

Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

Insert

PTEN (PTEN Human)

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Promoter

tet-on promoter

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pTK-Slug

Plasmid

#36986

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

slug (Snai2 Mouse)

Use

Lentiviral

Available Since

May 31, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-rTH-tTA

Plasmid

#133268

Purpose

An AAV genome that expresses tTA from the rat TH promoter

Depositor

Viviana Gradinaru

Article

Robinson et al Elife. 2019 Sep 23;8. pii: 48983. doi: 1

Insert

tTA

Use

AAV

Expression

Mammalian

Mutation

*(see below)

Promoter

rTH

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pPLtetO-FtsA

Plasmid

#107558

Purpose

Plasmid for titrating synthesis of FtsA with doxycycline

Depositor

Uwe Sauer

Article

Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

Insert

ftsA

Use

Synthetic Biology

Promoter

pLtetO

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pAF259

Plasmid

#105494

Purpose

pRPF185 derived plasmid encoding an anhydrotetracyclin inducible BitlucOpt

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

Insert

Bitlucopt

Use

Atc-dependent expression in c. difficile

Promoter

Ptet

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pTS1017-Tier1-OTetO7-PCMVmin-SEAP

Plasmid

#169586

Purpose

Tier-1 vector encoding PTetO7-driven SEAP (OTetO7-PCMVmin-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP expression

Expression

Mammalian

Promoter

TetO7-PCMVmin

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-TERF1

Plasmid

#222542

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of TERF1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

TERF1 (TERF1 Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pRG659_lexO-FseI_LexA-TF_LEU2MX

Plasmid

#154816

Purpose

Inducible expression of FseI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

FseI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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