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pMito-Electra2

Plasmid

#184927

Purpose

Mitochondrial labeling with blue fluorescent protein Electra2 fused to partial COX8A

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

COX8A-Electra2

Use

Tags

Electra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pIZ-Flag6His-BmAgo3

Plasmid

#50559

Purpose

Expresses FLAG, His tagged BmAgo3 in silkworm cell line BmN4 or insect cells

Depositor

Yukihide Tomari

Article

Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

Insert

BmAgo3

Use

Tags

6xHIS and Flag

Expression

Insect

Mutation

Promoter

OpIE2

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pCX-SponGee

Plasmid

#134775

Purpose

mammalian expression of SponGee, a buffer of cGMP that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

Insert

SponGee

Use

Tags

FLAG and mRFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Hygro DEST H2B-mRuby2

Plasmid

#90236

Purpose

Lentiviral vector to express H2B-mRuby2 under PGK promoter (With Hygromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Hygro DEST H2B-mCerulean3

Plasmid

#90234

Purpose

Lentiviral vector to express H2B-mCerulean3 under PGK promoter (With Hygromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCytERM-Electra2

Plasmid

#179477

Purpose

ER Visualization (OSER assay) Expresses Electra2 in fusion with N-terminal part of p450

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

CytERM-Electra2

Use

Tags

Electra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-CBS (WT)

Plasmid

#182923

Purpose

Mammalian expression plasmid for human CBS

Depositor

Philip Eaton

Article

Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

Insert

Cystathionine Beta-Synthase (CBS Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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HA-RIPLET ΔRING

Plasmid

#218506

Purpose

RIPLET lacking N-terminal RING domain in pcDNA3.1

Depositor

Sun Hur

Article

Cadena et al Cell. 2019 May 16;177(5):1187-1200.e16.

Insert

RIPLET delta RING domain (RNF135 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 11, 2024

Availability

Academic Institutions and Nonprofits only

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pTol2-nbt-Electra2-P2A-mScarlet

Plasmid

#196114

Purpose

zebrafish expression of nbt-Electra2-P2A-mScarlet

Depositor

Reinhard Köster

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

nbt-Electra2-P2A-mScarlet

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pEThT-SAHS1

Plasmid

#90025

Purpose

To express His-tagged SAHS1 in bacteria

Depositor

Takekazu Kunieda

Article

Yamaguchi et al PLoS One. 2012;7(8):e44209.

Insert

SAHS1

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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884 pGEX2T PTEN G129E

Plasmid

#10747

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

G129E

Promoter

Available since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pCS2-FLAG-hEcto/Tif1g

Plasmid

#20902

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (TRIM33 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal deletion of 37 aminoacids; Ectodermin …

Promoter

Available since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
pH2B-Electra1

Plasmid

#179478

Purpose

Nuclear labeling with blue fluorescent protein Electra1 fused to H2B

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

H2B-Electra1

Use

Tags

Electra1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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SpRY

Plasmid

#198544

Purpose

Expresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpRY

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pPaxillin-Electra2

Plasmid

#184926

Purpose

Expresses paxillin fused to blue fluorescent protein Electra2

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

paxillin-Electra2

Use

Tags

Electra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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qMaLioffG

Plasmid

#212825

Purpose

green fluorescent FLIM indicator for endogenous ATP imaging

Depositor

Tetsuya Kitaguchi

Article

Arai et al bioRxiv 2023.08.29.555275

Insert

qMaLioffG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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819 pGEX2T PTEN C124S

Plasmid

#10745

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

C124S

Promoter

Available since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pTol2-nbt-mTagBFP2-P2A-mScarlet

Plasmid

#196115

Purpose

zebrafish expression of nbt-mTagBFP2-P2A-mScarlet

Depositor

Reinhard Köster

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

nbt-mTagBFP2-P2A-mScarlet

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBR BsmAIM Kan + p15A BsaIR Amp

Plasmid

#206208

Purpose

Contains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIR

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

BsamAIM and BsaIR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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882 pSG5L HA PTEN G129E

Plasmid

#10746

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Use

Tags

Expression

Mammalian

Mutation

G129E

Promoter

Available since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only

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