We narrowed to 13,395 results for: mpl

1. pTP341 pHAGE2 CMV TagBFP-3xFLAG

   Plasmid

   #199391

   Purpose

   Lentiviral transfer plasmid for constitutive expression of a TagBFP-3xFLAG in mammalian cells

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Insert

   TagBFP-3xFLAG
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. His6_MBP_FXR2P_P474S_P493N

   Plasmid

   #198695

   Purpose

   Expression of human FXR2 protein in E. coli

   Depositor

   Simpson Joseph

   Article

   Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

   Insert

   FXR2P (FXR2 Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Changed proline 474 to serine and proline 493 asp…

   Promoter

   T7

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMBP-SpyCatcher

   Plasmid

   #79053

   Purpose

   Expresses maltose binding protein (MBP) SpyCatcher fusion

   Depositor

   James Bowie

   Article

   Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

   Insert

   MBP-SpyCatcher
   

   Use

   Tags

   6xHis and Maltose binding protein

   Expression

   Bacterial

   Mutation

   Derived from pDEST14-SpyCatcher (Plasmid #35044)…

   Promoter

   T7

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. ESCRT-I

   Plasmid

   #17634

   Depositor

   James Hurley

   Article

   Kostelansky et al Cell. 2006 Apr 7. 125(1):113-26.

   Insert

   Vps23(1-385) – His/Vps28(1-242) – Vps37(1-213) – Mvb12(1-101) (VPS28, STP22, SRN2 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. Col2.3deltaTK

   Plasmid

   #114273

   Purpose

   Conditional ablation of mature bone cells via ganciclovir exposure of cells expressing a truncated thymidine kinase.

   Depositor

   David Rowe

   Article

   Visnjic et al J Bone Miner Res. 2001 Dec;16(12):2222-3

   Insert

   Col2.3 promoter + deltaTK + BGH polyA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   2.3 kb rat type 1 collagen

   Available since

   Oct. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac1 INI1

   Plasmid

   #1953

   Depositor

   Robert Kingston

   Article

   Phelan et al Mol Cell 1999 Feb;3(2):247-53.

   Insert

   INI1 (SMARCB1 Human)
   

   Use

   Tags

   flag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pCMV5B-TGFbeta receptor I K232R

   Plasmid

   #11763

   Depositor

   Jeff Wrana

   Article

   Wieser et al EMBO J. 1995 May 15. 14(10):2199-208.

   Insert

   TGFbeta receptor I (TGFBR1 Human)
   

   Use

   Tags

   6xHIS

   Expression

   Mammalian

   Mutation

   K232R: kinase dead

   Promoter

   CMV

   Available since

   May 12, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)

   Plasmid

   #219537

   Purpose

   a. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotor

   Depositor

   Jeffrey Gerst

   Article

   Haimovich et al bioRxiv 2024.11.06.622258

   Inserts

   Cre recombinase (cre )
   NLS-tdMCP-GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pMaCTag-Z05

   Plasmid

   #120048

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with eGFP

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   eGFP
   

   Use

   Pcr template

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-MS2-HA-HsSmg5_T

    Plasmid

    #147552

    Purpose

    Mammalian Expression of HsSmg5

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsSmg5 (SMG5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. L1_UBE2:mS-Lt-Ck3

    Plasmid

    #136131

    Purpose

    L1 in position 3, mark cell outline with mScarletI

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpUBE2:mScarletI-Lti6b_3tNos-35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pLH-sgRNA-Sirius-4X(MS2-PP7)

    Plasmid

    #121941

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-4X(MS2-PP7)
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. hMTP

    Plasmid

    #124281

    Purpose

    Expresses human MTP in mammalian cells.

    Depositor

    Mahmood Hussain

    Article

    Sellers et al J Biol Chem. 2003 May 30;278(22):20367-7

    Insert

    MTTP (MTTP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. p210 pCMV-CREM

    Plasmid

    #8395

    Purpose

    Cre with chimeric/β-globin intron within the Cre coding sequence

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    CREM
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    modified Cre

    Promoter

    CMV

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-5X1 Flag WDR5

    Plasmid

    #15553

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    Wdr5 (Wdr5 Mouse)
    

    Use

    Tags

    Flag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

16. PCMV-intron myc Rab2WT

    Plasmid

    #46779

    Purpose

    expression of WT Rab2a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB2 (RAB2A Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

17. 3TERM_Nos-35S

    Plasmid

    #136116

    Purpose

    3TERM after CDS/ CDS12-CTAG

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    3TERM_Nos-35S (A. tumefaciens, CaMV)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pcDNA-RanWT-mRFP1-polyA

    Plasmid

    #59750

    Purpose

    Express wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Ran-mRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV MDM-2

    Plasmid

    #16441

    Depositor

    Bert Vogelstein

    Article

    Oliner et al Nature. 1992 Jul 2. 358(6381):80-3.

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSFDuet1-Cdc45

    Plasmid

    #126878

    Purpose

    Expresses human Cdc45 in bacteria

    Depositor

    Luca Pellegrini

    Article

    Simon et al Nat Commun. 2016 May 18;7:11638. doi: 10

    Insert

    CDC45 (CDC45 Human)
    

    Use

    Tags

    No tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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