We narrowed to 10,822 results for: nls

1. pMP597

   Plasmid

   #134999

   Purpose

   Inducible ZF-m6A Writer. Use abscisic acid (ABA) to direct GATC methylation to ZF binding sites. pMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastR

   Depositor

   Ahmad Khalil

   Article

   Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

   Insert

   pMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastR
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ166-iSpyMac

   Plasmid

   #149553

   Purpose

   Gateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMs

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

   Insert

   iSpyMacCas9
   

   Use

   CRISPR; Gateway compatible zispymaccas9 entry clo…

   Tags

   3X FLAG, NLS and NLS

   Expression

   Plant

   Mutation

   R221K, N394K, Protospacer interacting domain form…

   Available Since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-dLbCpf1-BE-YEE

   Plasmid

   #154148

   Purpose

   Expresses dLbCpf1-BE-YEE(dLbCas12a-BE-YEE) in mammalian cells.

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

   Insert

   dLbCpf1-BE-YEE (dCas12a-BE-YEE)
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   APOBEC1(W90Y+R126E+R132E), dLbCpf1(D832A+E925A+D1…

   Promoter

   CMV

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTriEx-HTNC

   Plasmid

   #13763

   Purpose

   For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT promotes cellular uptake of recombinant Cre into mammalian cells.

   Depositor

   Klaus Rajewsky

   Article

   Peitz et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99

   Insert

   His-TAT-NLS-Cre
   

   Use

   Baculovirus

   Tags

   His Tag, NLS, and TAT

   Expression

   Bacterial

   Promoter

   p10 promoter

   Available Since

   April 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. SauCas9 PE2*

   Plasmid

   #169851

   Purpose

   Mammalian Expression, sauCas9 based prime editor

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SaCas9-N580A-M-MLV
   

   Tags

   bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-eBE-S3

   Plasmid

   #101093

   Purpose

   Expresses eBE-S3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

   Insert

   eBE-S3
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-PE2-SpG (HES824)

   Plasmid

   #242689

   Purpose

   Prime editing in mammalian cells with SpG-based PE2 prime editors

   Depositor

   Benjamin Kleinstiver

   Insert

   pCMV-NLS-SpCas9(H840A)-M-MLV-RT(D200N,T306K,W313F,T330P,L603W)-NLS
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   PE2 mutations in MMLV RT, SpG mutations in SpCas9…

   Promoter

   CMV

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-sadCas9-VP64

   Plasmid

   #115790

   Purpose

   An AAV vector expressing S. aureus dCas9 fused to VP64.

   Depositor

   Nadav Ahituv

   Article

   Matharu et al Science. 2018 Dec 13. pii: science.aau06

   Insert

   Sa-dCas9
   

   Use

   AAV

   Tags

   NLS and NLS-VP64

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Nme2Cas9_AAV

   Plasmid

   #119924

   Purpose

   All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   Nme2Cas9
   

   Use

   AAV

   Tags

   NLS-3xHA-NLS and NLS-NLS

   Expression

   Mammalian

   Promoter

   U1a

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. SauKKHCas9 PE2*

    Plasmid

    #169852

    Purpose

    Mammalian Expression, sauCas9-KKH based prime editor

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SaCas9KKH-N580A-M-MLV
    

    Tags

    bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. HBT_pcoCASphi_version1

    Plasmid

    #197931

    Purpose

    Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Insert

    pcoCasphi-2
    

    Use

    CRISPR

    Tags

    FLAG tag and SV40 NLS and nucleoplasmin NLS

    Expression

    Plant

    Promoter

    35SPPDK promoter

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. MTK3b_030

    Plasmid

    #123808

    Purpose

    Encodes NLS::dCAS9::NLS as a Type 3b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    NLS::dCAS9::NLS
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. BII-tdT-inv-GFP

    Plasmid

    #133393

    Purpose

    Piggybac vector for bidirectional promoter reporter assay

    Depositor

    Blair Madison

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    No promoter

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. BII-ChPtW-smURFP

    Plasmid

    #133399

    Purpose

    Piggybac vector for constitutive expression of smURFP-NLS

    Depositor

    Blair Madison

    Insert

    smURFP-NLS
    

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6-SacB-Cas9-T2A-mCherry_(BbsI)

    Plasmid

    #117070

    Purpose

    Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection

    Depositor

    Kim Failor

    Inserts

    Cas9
    SacB
    

    Use

    CRISPR

    Tags

    3x FLAG, NLS, and T2A-mCherry

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT22iuasvApoWtEos3N(#486)

    Plasmid

    #184107

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-2nls-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transgenesis (blue eyes)

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    4xUAS

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. BII-ChPtW-iRFP670

    Plasmid

    #133397

    Purpose

    Piggybac vector for constitutive expression of iRFP670-NLS

    Depositor

    Blair Madison

    Insert

    iRFP-670-NLS
    

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pY125 (pcDNA3.1-huBfCpf1)

    Plasmid

    #92301

    Purpose

    Expression of humanized BfCpf1

    Depositor

    Feng Zhang

    Insert

    huBfCpf1
    

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Available Since

    June 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pY115 (pcDNA3.1-huMlCpf1)

    Plasmid

    #92275

    Purpose

    Expression of humanized MlCpf1

    Depositor

    Feng Zhang

    Insert

    huMlCpf1
    

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Available Since

    June 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pY112 (pcDNA3.1-huSaCpf1)

    Plasmid

    #92269

    Purpose

    Expression of humanized SaCpf1

    Depositor

    Feng Zhang

    Insert

    huSaCpf1
    

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Available Since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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