Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 6,354 results for: nls

Showing: 3821 - 3840 of 6354 results

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pEPOR0SP0008

Plasmid

#117535

Purpose

Level0 Golden Gate part: CDS, AsCas12a

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

AsCas12a (with stop codon)

Use

CRISPR

Tags

3xHA and NLS

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pDS2

Plasmid

#70135

Purpose

CEN/ARS yeast shuttle vector with DS2 selection marker

Depositor

Morten Sommer

Article

Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

Insert

DS2

Use

Synthetic Biology

Promoter

AgTEF1

Available Since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pGL4.23-CD69 CaRE3 (minus strand)

Plasmid

#91845

Purpose

Luciferase reporter for CD69 enhancer (IGI-P0622)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

CD69 CaRE3 (minus strand) (CD69 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Missing 1 base from 13 base polyT tract beginning…

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE4 (plus strand)

Plasmid

#91850

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0345)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE4 (plus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE4 scramble (plus strand)

Plasmid

#91852

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0347)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE4 scramble (plus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Sequence scrambled from ch10:6094588-6094934

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE4 (minus strand)

Plasmid

#91849

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0626)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE4 (minus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE1 (plus strand)

Plasmid

#91837

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0614)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE1 (plus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-CD69 CaRE3 (plus strand)

Plasmid

#91844

Purpose

Luciferase reporter for CD69 enhancer (IGI-P0621)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

CD69 CaRE3 (plus strand) (CD69 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Missing 1 base from 13 base polyT tract beginning…

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE5 (plus strand)

Plasmid

#91841

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0618)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE5 (plus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE3 (minus strand)

Plasmid

#91835

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0612)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE3 (minus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

rs7893324 A>G, rs7090530 G>T, rs7090512 A&g…

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE4 SNP (plus strand)

Plasmid

#91851

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0346)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE4 SNP (plus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

rs61839660 C>T

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE5 (minus strand)

Plasmid

#91836

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0613)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE5 (minus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE2 (plus strand)

Plasmid

#91846

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0623)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE2 (plus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

rs6602425 T>G, T>C at chr10:6117603

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE2 (minus strand)

Plasmid

#91847

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0624)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE2 (minus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

rs6602425 A>C, G>A mutation at chr10:611706…

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE6 (minus strand)

Plasmid

#91840

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0617)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE6 (minus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

rs7893467 C>A

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE6 (plus strand)

Plasmid

#91839

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0616)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE6 (plus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

rs7893467 G>T, rs11256448 A>G

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE1 (minus strand)

Plasmid

#91838

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0615)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE1 (minus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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CRE recombinase

Plasmid

#62730

Purpose

Expresses Cre recombinase protein in bacterial cells

Depositor

Niels Geijsen

Article

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

Cre recombinase

Use

Cre/Lox

Tags

His tag and NLS

Expression

Bacterial

Mutation

Codon optimazed for e.coli

Promoter

T7

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-HypaCAS9-pA

Plasmid

#131467

Purpose

Expresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Ikeda et al Commun Biol. 2019 Oct 10;2:371. doi: 10.

Insert

Codon optimized HypaCas9

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFC332

Plasmid

#87845

Purpose

AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

Depositor

Uffe Mortensen

Article

Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

Inserts

Cas9
hph (hygromycin resistance marker)

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

Aspergillus nidulans tef1 promoter and Aspergillu…

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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Showing: 3821 - 3840 of 6354 results

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