We narrowed to 24,276 results for: Sis

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pOTTC840 - pAAV 5xATF6 secNLuc

Plasmid

#82497

Purpose

Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

Depositor

Brandon Harvey

Article

Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

Insert

secNLuc

Use

AAV

Expression

Mammalian

Promoter

5x(ATF6 binding site) c-Fos minimal promoter

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pKan-PCUP1-AID*(N)-9myc

Plasmid

#99526

Purpose

N-terminal AID*-9myc degron cassette with CUP1 promoter and KanR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Promoter

CUP1

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pHEE901-A3A

Plasmid

#229044

Purpose

nCas9 mediated-base editing with NGG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A in Cas9.

Depositor

Huawei Zhang

Article

Wang et al Genome Biol. 2024 Aug 9;25(1):215. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pMax_mOrange_A215C

Plasmid

#177828

Purpose

Site-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mOrange_A215C

Expression

Mammalian

Mutation

A215C

Promoter

CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMax_GFP_C289T

Plasmid

#177826

Purpose

Site-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

GFP_C289T

Expression

Mammalian

Mutation

C289T

Promoter

CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Gal4BD-CRY2(535)

Plasmid

#75369

Purpose

Yeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 (CRY2 Mustard Weed)

Tags

Gal4BD

Expression

Yeast

Promoter

ADH1

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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hHSF1 K80Q

Plasmid

#118351

Purpose

Plasmid expresses hHSF1 with K80 in DBD mutated to Q, which abolish DNA-binding activity of HSF1.

Depositor

Lea Sistonen

Article

Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K80Q

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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HBT-CPK32-Flag

Plasmid

#107432

Purpose

For Arabidopsis mesophyll protoplast transient expression assay

Depositor

Jen Sheen

Article

Liu et al Nature. 2017 May 18;545(7654):311-316. d

Insert

CPK32 (CPK32 Mustard Weed)

Tags

Flag

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pMax_BFP_A191G

Plasmid

#177823

Purpose

Site-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

tagBFP_A191G

Expression

Mammalian

Mutation

A191G

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pTRE-Tight-FLAG-CYLD-WT

Plasmid

#60030

Purpose

WT CYLD under control of Tet promoter with N-terminal FLAG tag

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Tags

FLAG

Expression

Mammalian

Promoter

TRE

Available Since

Nov. 25, 2014

Availability

Academic Institutions and Nonprofits only
pQE-BR1

Plasmid

#160333

Purpose

This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is improved compared to wild type.

Depositor

Sang Taek Jung

Article

Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12

Insert

BR1

Tags

6xHis tag

Expression

Bacterial

Promoter

T5

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKan-PCUP1-9myc-AID*(N)

Plasmid

#99527

Purpose

N-terminal 9myc-AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-117) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Promoter

CUP1

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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mRFP-mem

Plasmid

#55622

Purpose

This red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mRFP.

Depositor

Catherine Berlot

Article

Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

Insert

mRFP-Mem

Tags

The N-terminal 20 residues of neuromodulin are fu…

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-AtUBC8-S-AtUBA1-S

Plasmid

#222714

Purpose

Expression AtUBA1-S and AtUBC8-S in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Inserts

AtUBC8 (UBC8 Mustard Weed)
ATUBA1 (UBA1 Mustard Weed)

Tags

S-Tag

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pKan-PRFA1-9myc-AID*(N)

Plasmid

#99528

Purpose

N-terminal 9myc-AID* degron cassette with RFA1 promoter and KanR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-117) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Promoter

RFA1

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-HA-ERK5

Plasmid

#53175

Purpose

Expresses HA tagged human ERK5 from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK5 (MAPK7 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
PBE-NG

Plasmid

#229045

Purpose

Modified nCas9 mediated-base editing with NG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R in Cas9.

Depositor

Huawei Zhang

Article

Wang et al Genome Biol. 2024 Aug 9;25(1):215. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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Myc-CYLD-C601A

Plasmid

#60027

Purpose

Myc-tagged CYLD catalytic inactive (C601A) expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Tags

Myc

Expression

Mammalian

Mutation

Cysteine 601 changed to Alanine

Promoter

CMV

Available Since

Nov. 25, 2014

Availability

Academic Institutions and Nonprofits only

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3xFLAG-dCas9/MSCV-EGFP

Plasmid

#82613

Purpose

Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujita et al Genes Cells. 2018 Feb 26. doi: 10.1111/g

Insert

3xFLAG-dCas9

Use

Retroviral

Tags

3xFLAG tag and NLS (nuclear localization signal)

Mutation

human codon-optimized, D10A + H840A

Promoter

LTR

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pSNV2 CHER NLS cop1at(335-675)

Plasmid

#44972

Depositor

Thanos Halazonetis

Article

Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

Insert

mCherry-NLS-COP1 (COP1 Mustard Weed)

Tags

NLS and mCherry

Expression

Mammalian

Mutation

deleted amino acids 1-334

Promoter

pCMV IE

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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