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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 30,838 results for: gfp

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Showing: 3841 - 3860 of 30838 results

  3. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II

    Plasmid
    #52092
    Purpose
    Retroviral expression of all four WT CD3 subunits, co-expressed with EGFP
    Depositor
    Dario Vignali
    Publication
    Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:
    Inserts
    CD3 delta (Cd3d Mouse)
    CDC gamma (Cd3g Mouse)
    CD3 epsilon (Cd3e Mouse)
    CD3 zeta (Cd247 Mouse)
    Use
    Retroviral
    Tags
    F2A, IRES-EGFP, P2A, and T2A
    Expression
    Mammalian
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52092-plasmid-map-sequence-id-80506

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  5. NMD2

    Plasmid
    #216220
    Purpose
    Nonsense mediated decay GFP P2A RFP reporter with two b-globin introns
    Depositor
    Rebecca Voorhees
    Publication
    Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP P2A RFP_b-globin intron x2
    Expression
    Mammalian
    Available since
    April 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    216220-plasmid-map-sequence-id-429914

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  8. pcDNA3-GFP-GFP-FLARE-AKAR

    Plasmid
    #123332
    Purpose
    Green-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.
    Depositor
    Jin Zhang
    Publication
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP-GFP-FLARE-AKAR
    Tags
    6xHIS, EGFP, T7 tag (gene 10 leader), and Xpress …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    123332-plasmid-map-sequence-id-306919

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  10. pREF-GFP kan

    Plasmid
    #122061
    Purpose
    reporter plasmid for termination and terminator override to express GFP. Marked with URA3
    Depositor
    Daniel Reines
    Publication
    Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)
    Inserts
    intergenic terminator from yeast IMD2
    green fluorescent protein
    Expression
    Yeast
    Promoter
    GAL1 and GAL1 comes in pYES2
    Available since
    Feb. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    122061-plasmid-map-sequence-id-236157

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  11. pCAG-nucGFP11-3xControlgRNA

    Plasmid
    #224568
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Publication
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    GFP11-H2B
    Use
    CRISPR
    Tags
    Histone H2B (nuclear localization)
    Mutation
    Codon optimized
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224568-plasmid-map-sequence-id-451496

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  14. SeeSaw Reporter (SSR2.0)

    Plasmid
    #50393
    Purpose
    SSR2.0 has two target sites in opposite orientation for generate only one cycle of repairing after I-SceI endonuclease expression.
    Depositor
    Pablo Huertas Sánchez
    Publication
    Gomez-Cabello et al PLoS One. 2013 Oct 14;8(10):e77206. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SSR2.0
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50393-plasmid-map-sequence-id-271055

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  17. pCAG-3xGFP11-mem-3xControlgRNA

    Plasmid
    #224583
    Purpose
    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Publication
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    3xGFP11
    Use
    CRISPR
    Tags
    Membrane Localization Signal
    Mutation
    Codon optimized
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224583-plasmid-map-sequence-id-451495

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  18. JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

    Plasmid
    #104567
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
    Use
    CRISPR
    Tags
    NLS-3xHA-VPR
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    Jan. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104567-plasmid-map-sequence-id-201429

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Showing: 3841 - 3860 of 30838 results