We narrowed to 87,860 results for: lias

1. PH-miRFP-FRB

   Plasmid

   #193568

   Purpose

   Expresses PH domain from PLC delta1 tagged with miRFP703 and FRB mutant (T2098L) in mammalian cells.

   Depositor

   Justin Taraska

   Article

   Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

   Insert

   PH domain from PLC delta1 (Plcd1 Rat)
   

   Use

   Tags

   FRB (T2098L) and miRFP703

   Expression

   Mammalian

   Mutation

   PH domain

   Promoter

   CMV

   Available since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-CB1R-V2tail-TevN-BLITz1-TetR-VP16

   Plasmid

   #89872

   Purpose

   expresses CB1R-iTango component in mammalian cells

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   CB1R-V2tail-TevN-BLITz1-TetR-VP16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLJM1-CRY2-mCherry-(GGGGS)3-hRGS2box

   Plasmid

   #112257

   Purpose

   Expresses RGS component of opto-RGS2 in mammalian cells

   Depositor

   Brian Chow

   Article

   Hannanta-Anan et al ACS Synth Biol. 2018 Jun 15;7(6):1488-14

   Insert

   CRY2-mCherry-(GGGGS)3-hRGS2box
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry_LD 0_CS G_tTA-BFP

   Plasmid

   #58856

   Purpose

   MESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   MESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
   

   Use

   AAV

   Tags

   EBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3HA-AMOT p130∆PPxY1

   Plasmid

   #166711

   Purpose

   expresses AMOT with inactivating mutation in first PPxY motif in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   Angiomotin p130 L129A P130A
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   changed leucine 129 to alanine, proline 130 to al…

   Promoter

   CMV promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-mAkt-ER

   Plasmid

   #39530

   Depositor

   Julian Downward

   Article

   Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

   Insert

   Akt (Akt1 Mouse)
   

   Use

   Tags

   ER

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA-HA-hTRAF3 88-C

   Plasmid

   #44034

   Depositor

   Shao-Cong Sun

   Article

   Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

   Insert

   TNF receptor-associated factor 3 (TRAF3 Human)
   

   Use

   Tags

   1xHA

   Expression

   Mammalian

   Mutation

   Contains amino acids 89-568; V274D and S393G

   Promoter

   Available since

   March 27, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pDx_mScarlet-I-SYFP2

   Plasmid

   #189761

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mScarlet-I-SYFP2 fusion protein for FRET analysis

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet-I-SYFP2
   

   Use

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV & Rha

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. dTomato_LD 0_CS M_tTA-BFP

   Plasmid

   #58875

   Purpose

   MESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   MESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
   

   Use

   AAV

   Tags

   EBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-Akt(R25C)

    Plasmid

    #39532

    Depositor

    Julian Downward

    Article

    Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

    Insert

    Akt(R25C) (Akt1 Mouse)
    

    Use

    Tags

    6G linker, EGFP, and HA

    Expression

    Mammalian

    Mutation

    R25C

    Promoter

    Available since

    Sept. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3_HLA-cmyc-GRM3 C127A

    Plasmid

    #140451

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK-flag-USP19 494-1318

    Plasmid

    #78587

    Purpose

    Expression flag-USP19 494-1318 in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-USP19 494-1318 (USP19 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-493

    Promoter

    CMV

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pLL7.0: Venus-oLID-Mito (From ActA)

    Plasmid

    #60414

    Purpose

    Mammalian Expression of Venus-oLID-Mito

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    Venus-oLID-Mito
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP_E1770Q-talin1 (1-2541)

    Plasmid

    #166121

    Purpose

    For expression of full-length mouse talin-1 carrying mutation E1770Q in mammalian cells. Contains C-terminal EGFP tag.

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    E1770Q-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    E1770Q

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. optoPKAW196R/F327A

    Plasmid

    #104547

    Purpose

    Expresses Cry2-mCherry-PKA W196R/F327A in mammalian cells.

    Depositor

    David Lawrence

    Article

    O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

    Insert

    Cry2-mCherry-PKA W196R/F327A
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    PKA W196R and Y204A

    Promoter

    CMV

    Available since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKF-D2irTNP2AG-T2APuroR-5kwh

    Plasmid

    #128255

    Purpose

    Mammalian linearizer gene circuit based on negative feedback in a Flp-In expression system controlling the PuroR gene.

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    hTetR::P2A::EGFP::T2A::PuroR
    

    Use

    Synthetic Biology; Flp-in expression vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-D2ir promoter

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcdna3 T7 Jip1b

    Plasmid

    #52122

    Purpose

    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

    Insert

    JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

18. FnoCas12a-DR-cr in PBCSK-

    Plasmid

    #126642

    Purpose

    Cloning vector FnoCas12a DR-crRNA for expression in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    FnoCas12a Full Length Direct Repeat crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLL7.0: Venus-oLID-CAAX (from KRas4B)

    Plasmid

    #60412

    Purpose

    Mammalian Expression of Venus-oLID-CAAX

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    Venus-oLID-CAAX
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mSTIM1_1-310-CFP

    Plasmid

    #69551

    Purpose

    Expresses a STIM1_1-310 fragment mammalian cells

    Depositor

    Yubin Zhou

    Article

    Ma et al Nat Commun. 2015 Jul 17;6:7826. doi: 10.

    Insert

    mSTIM1_1-310
    

    Use

    Tags

    Cyan Fluorescent Protein (CFP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only