We narrowed to 4,523 results for: ARA-2

1. pX459-HypaCas9-mR2-ECT2_sgRNA

   Plasmid

   #183875

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ECT2 sgRNA spacer (ECT2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-hPIKfyve

   Plasmid

   #121148

   Purpose

   Expresses GFP-tagged human PIKfyve in mammalian cells.

   Depositor

   Geert van den Bogaart

   Article

   Baranov et al iScience. 2019 Jan 25;11:160-177. doi: 1

   Insert

   1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. mEos3.2FPP

   Plasmid

   #203758

   Purpose

   Encodes the membrane anchor of HRas, including the last 10 residues of the C terminus with 1 farnesylation and 2 palmitoylations, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   HRas c-term (HRAS Human)
   

   Tags

   mEos3.2

   Expression

   Mammalian

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. proUBI10::VIH2-FL::FLAG-H505A-H741A-H975A

   Plasmid

   #238337

   Purpose

   proUBI10::VIH2-FL::FLAG-H505A-H741A-H975A ubiquitous expression in Arabidopsis plants

   Depositor

   Michael Hothorn

   Article

   Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

   Insert

   VIP1 (VIP1 Mustard Weed)
   

   Tags

   3x Flag-tag

   Expression

   Plant

   Mutation

   AtVIH2-H505A-H741A-H975A

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. proUBI10::VIH2-FL::FLAG-R372A-H373A-R376A

   Plasmid

   #238333

   Purpose

   proUBI10::VIH2-FL::FLAG-R372A-H373A-R376A ubiquitous expression in Arabidopsis plants

   Depositor

   Michael Hothorn

   Article

   Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

   Insert

   VIP1 (VIP1 Mustard Weed)
   

   Tags

   3x Flag-tag

   Expression

   Plant

   Mutation

   AtVIH2-R372A-H373A-R376A

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. proUBI10::VIH2-FL::FLAG-P378V-G500V-G501A

   Plasmid

   #238338

   Purpose

   proUBI10::VIH2-FL::FLAG-P378V-G500V-G501A ubiquitous expression in Arabidopsis plants

   Depositor

   Michael Hothorn

   Article

   Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

   Insert

   VIP1 (VIP1 Mustard Weed)
   

   Tags

   3x Flag-tag

   Expression

   Plant

   Mutation

   AtVIH2-P378V-G500V-G501A

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. proUBI10::VIH2-FL::FLAG-R722S-D724A-T726A

   Plasmid

   #238339

   Purpose

   proUBI10::VIH2-FL::FLAG-R722S-D724A-T726A ubiquitous expression in Arabidopsis plants

   Depositor

   Michael Hothorn

   Article

   Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

   Insert

   VIP1 (VIP1 Mustard Weed)
   

   Tags

   3x Flag-tag

   Expression

   Plant

   Mutation

   AtVIH2-R722S-D724A-T726A

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. proUBI10::VIH2-FL::FLAG-K383A-K447A-K598A-R722A

   Plasmid

   #238340

   Purpose

   proUBI10::VIH2-FL::FLAG-K383A-K447A-K598A-R722A ubiquitous expression in Arabidopsis plants

   Depositor

   Michael Hothorn

   Article

   Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

   Insert

   VIP1 (VIP1 Mustard Weed)
   

   Tags

   3x Flag-tag

   Expression

   Plant

   Mutation

   AtVIH2-K383A-K447A-K598A-R722A

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. proUBI10::VIH2-FL::FLAG-R372A-H373A-R376A-E882A

   Plasmid

   #238334

   Purpose

   proUBI10::VIH2-FL::FLAG-R372A-H373A-R376A-E882A ubiquitous expression in Arabidopsis plants

   Depositor

   Michael Hothorn

   Article

   Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

   Insert

   VIP1 (VIP1 Mustard Weed)
   

   Tags

   3x Flag-tag

   Expression

   Plant

   Mutation

   AtVIH2-R372A-H373A-R376A-E882A

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. proUBI10::VIH2-FL::FLAG-K379A-K381A-K498A

    Plasmid

    #238341

    Purpose

    proUBI10::VIH2-FL::FLAG-K379A-K381A-K498A ubiquitous expression in Arabidopsis plants

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Mustard Weed)
    

    Tags

    3x Flag-tag

    Expression

    Plant

    Mutation

    AtVIH2-K379A-K381A-K498A

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-4T1_GST-GBRP

    Plasmid

    #223728

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    GABARAP (GABARAP Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 2-116 only, with Glycine exposed

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCOG40-HRS (257-276) Hs

    Plasmid

    #83236

    Purpose

    pGST-HRS (257-276) Hs, Expresses the GST-HRS in E.coli

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Insert

    HRS (257-276) Hs (HGS Human)
    

    Expression

    Bacterial

    Mutation

    258-277

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 Flag Cl-sensitive YFP hNKCC1 WT (NT13)

    Plasmid

    #49060

    Purpose

    Expresses human NKCC1 with an N-terminal 3xFlag-YFP-(chloride-sensing) tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Tags

    3x Flag and YFP (chloride-sensitive)

    Expression

    Mammalian

    Mutation

    Cl-sensitive YFP (EYFP/ V163S A206K) in hNKCC1 in…

    Promoter

    CMV

    Available Since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV-EVA1ext-hFcIgG3-IRES-YFP

    Plasmid

    #64926

    Purpose

    To express extracellular portion of EVA1 (mouse) fused with human Fc(IgG3)

    Depositor

    Pau Serra

    Article

    Garabatos et al Monoclon Antib Immunodiagn Immunother. 2

    Insert

    mouse EVA1 fused with human FcIgG3 (Mpzl2, IGHG3 Mouse, Human)
    

    Use

    Retroviral

    Tags

    IRES-YFP

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations (NT51)

    Plasmid

    #49061

    Purpose

    Expresses human NKCC1 mutated Ser and Thr residues in the N-terminus and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    S150N, S155Q, S170Q, T177A, S183R, S193E, T203A, …

    Promoter

    CMV

    Available Since

    Nov. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. BAM2 X050_pECIA14

    Plasmid

    #114904

    Purpose

    Prey vector BAM2 X050_pECIA14 should be used with bait vector BAM2 X050_pECIA2.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT3G49670 (BAM2 Mustard Weed)
    

    Expression

    Insect

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAC-VIOL

    Plasmid

    #53087

    Purpose

    Produces the carotenoid violaxanthin in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Neuman et al Plant J. 2014 Apr;78(1):80-93. doi: 10.1

    Insert

    zep (ABA1 Mustard Weed)
    

    Use

    Low copy number bacterial cloning vector

    Mutation

    Lacks codons for the first 60 N-terminal amino ac…

    Promoter

    T7

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. SKM2 X057_pECIA2

    Plasmid

    #115114

    Purpose

    Bait vector SKM2 X057_pECIA2 should be used with prey vector SKM2 X057_pECIA14.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT5G56040 (AT5G56040 Mustard Weed)
    

    Tags

    Fc, V5 and hexahistidine tags

    Expression

    Insect

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLS2 FLS2_pECIA2

    Plasmid

    #115120

    Purpose

    Bait vector FLS2 FLS2_pECIA2 should be used with prey vector FLS2 FLS2_pECIA14.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT5G46330 (FLS2 Mustard Weed)
    

    Tags

    Fc, V5 and hexahistidine tags

    Expression

    Insect

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. CEPR2 X048_pECIA2

    Plasmid

    #115099

    Purpose

    Bait vector CEPR2 X048_pECIA2 should be used with prey vector CEPR2 X048_pECIA14.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT1G72180 (AT1G72180 Mustard Weed)
    

    Tags

    Fc, V5 and hexahistidine tags

    Expression

    Insect

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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