We narrowed to 4,215 results for: Bre;

1. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

   Plasmid

   #98973

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 2 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQZ210

   Plasmid

   #106414

   Purpose

   Co-expresses trans SNARE complex with pDC183 in E.coli

   Depositor

   Axel Brunger

   Article

   Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

   Inserts

   Rat Synaptobrevin-2 fragment 28-66 (Vamp2 Synthetic)
   Syntaxin-1A fragment 191-256 (Stx1a Rat, Synthetic)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Fragment 191-256 , Codon optimization and Fragmen…

   Promoter

   T7

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRRL-VinculinVenus-S1033A

   Plasmid

   #211896

   Purpose

   Vinculin (chicken) with S1033 unphosphorylatable point mutation (S1033A) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.

   Depositor

   Brenton Hoffman

   Article

   Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12

   Insert

   VinculinVenus-S1033A (VCL Chicken)
   

   Use

   Lentiviral

   Tags

   VenusA206K

   Expression

   Mutation

   mutated vinculin serine 1033 to alanine (S1033A),…

   Promoter

   CMV

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRRL-VinculinVenus-S1033D

   Plasmid

   #211897

   Purpose

   Vinculin (chicken) with S1033 phosphomimetic point mutation (S1033D) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.

   Depositor

   Brenton Hoffman

   Article

   Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12

   Insert

   VinculinVenus-S1033D (VCL Chicken)
   

   Use

   Lentiviral

   Tags

   VenusA206K

   Expression

   Mutation

   mutated vinculin serine 1033 to aspartic acid (S1…

   Promoter

   CMV

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SNX12-PX (1-162)

   Plasmid

   #119093

   Purpose

   Bacterial expression of human phox homology (PX) domain, SNX12-PX (1-162)

   Depositor

   Brett Collins

   Article

   Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

   Insert

   SNX12-PX (1-162) (SNX12 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. SNX11-PX (7-167)

   Plasmid

   #119092

   Purpose

   Bacterial expression of human phox homology (PX) domain, SNX11-PX (7-167)

   Depositor

   Brett Collins

   Article

   Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

   Insert

   SNX11-PX (7-167) (SNX11 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTol2CG2-QUASM1:mScarletNLS-SV40pA

   Plasmid

   #155124

   Purpose

   Tol2 vector containing QUASM1 reporter element upstream of mScarlet-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   QUASM1:mScarletNLS
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   QUASM1-E1b

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1

   Plasmid

   #98976

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 1 and Cas9(N863A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-EF1a-delta-TMD-VAMP1-IRES-Hygromycin

   Plasmid

   #134866

   Purpose

   Expresses EGFP-tagged mutant of VAMP1

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Vesicle-associated membrane protein 1 (VAMP1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 97-118

   Promoter

   EF1a

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. SNX3-PX (1-162)

    Plasmid

    #119084

    Purpose

    Bacterial expression of human phox homology (PX) domain, SNX3-PX (1-162)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SNX3-PX (1-162) (SNX3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SGK3-PX (1-126)

    Plasmid

    #119117

    Purpose

    Bacterial expression of human phox homology (PX) domain, SGK3-PX (1-126)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SGK3-PX (1-126) (SGK3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQTEV-EPSTI1

    Plasmid

    #34826

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    epithelial stromal interaction 1 (EPSTI1 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SNX31-PX (1-106)

    Plasmid

    #119110

    Purpose

    Bacterial expression of human phox homology (PX) domain, SNX31-PX (1-106)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SNX31-PX (1-106) (SNX31 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SNX27-PX (156-265)

    Plasmid

    #119106

    Purpose

    Bacterial expression of human phox homology (PX) domain, SNX27-PX (156-265)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SNX27-PX (156-265) (SNX27 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEW66

    Plasmid

    #232794

    Purpose

    COMPASS Fragment 1 (Cps60, Cps50, Cps35, Cps25, Cps15) in PBIG1a

    Depositor

    Cynthia Wolberger

    Article

    Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1

    Insert

    COMPASS Fragment 1 (Cps60, Cps50, Cps35, Cps25, Cps15) (SWD1, BRE2, SWD2, SDC1, SHG1 Budding Yeast)
    

    Use

    Tags

    No tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. L2125-FRB-GPA-NLuc-CyOFP1

    Plasmid

    #222885

    Purpose

    Positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, 10 GS linker, CyOFP1.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-20GS-NanoLuc-10GS-CyOFP1
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-VcnTS-E1015A

    Plasmid

    #213415

    Purpose

    Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).

    Depositor

    Brenton Hoffman

    Article

    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

    Insert

    VcnTS-E1015A (VCL Synthetic, Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutated vinculin glutamic acid 1015 to alanine (E…

    Promoter

    CMV

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-VcnTS-E1021A

    Plasmid

    #213416

    Purpose

    Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).

    Depositor

    Brenton Hoffman

    Article

    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

    Insert

    VcnTS-E1021A (VCL Synthetic, Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutated vinculin glutamic acid 1021 to alanine (E…

    Promoter

    CMV

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-VcnCS-E1015A

    Plasmid

    #213412

    Purpose

    Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).

    Depositor

    Brenton Hoffman

    Article

    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

    Insert

    VcnCS-E1015A (VCL Synthetic, Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutated vinculin glutamic acid 1015 to alanine (E…

    Promoter

    CMV

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-VcnCS-E1021A

    Plasmid

    #213413

    Purpose

    Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).

    Depositor

    Brenton Hoffman

    Article

    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

    Insert

    VcnCS-E1021A (VCL Synthetic, Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutated vinculin glutamic acid 1021 to alanine (E…

    Promoter

    CMV

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge