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pLPCX cyto Grx1-roGFP2

Plasmid

#64975

Purpose

Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

Depositor

Tobias Dick

Article

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pJAM1.19_PB-CIT-PGK-Blast

Plasmid

#205564

Purpose

Express human CIT

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

CIT (CIT Human)

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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mEGFP-HRas

Plasmid

#18662

Depositor

Karel Svoboda

Article

Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

Insert

H-Ras (HRAS Human)

Tags

monomeric EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-2E2-HA-mEGFP

Plasmid

#129595

Purpose

The encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

Depositor

Tim Stasevich

Article

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pHage2-EF1a-TERT-IRES-Puro

Plasmid

#182590

Purpose

expresses TERT in mammalian cells

Depositor

Agnel Sfeir

Article

Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

Insert

hTERT (TERT Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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ColorfulCell

Plasmid

#62449

Purpose

MXS_chaining vector with 6 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMV::GTS-Citrine-bGHpA
CMV::Lyn-Ceruleanx3-bGHpA
CMV::NLS-TagBFPx3-bGHpA
CMV::MTS-AzamiGreen-bGHpA
CMV::iRFP670-SKL-bGHpA
CMV::ETS-mCherry-KDEL-bGHpA

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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TUBB5-Halo

Plasmid

#64691

Purpose

Expresses beta-tubulin labeled with Halo-tag

Depositor

Yasushi Okada

Article

Uno et al Nat Chem. 2014 Aug;6(8):681-9. doi: 10.1

Insert

TUBB

Tags

Halo-tag

Expression

Mammalian

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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N1-Tq-Ca-FLITS

Plasmid

#191454

Purpose

Genetically encoded calcium sensor (GECI) Tq-Ca-FLITS that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

Tq-Ca-FLITS

Expression

Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pK18msB

Plasmid

#177839

Purpose

Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size.

Depositor

Christopher Johnson

Article

Ling et al Nat Commun. 2022 Aug 22;13(1):4925. doi:

Type

Empty backbone

Use

Bacterial gene replacement

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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Pyronic/pcDNA3.1(-)

Plasmid

#51308

Purpose

Pyronic, FRET sensor that allow quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resolution.

Depositor

Luis Felipe Barros

Article

San Martin et al PLoS One. 2014 Jan 21;9(1):e85780. doi:

Insert

Pyronic

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3

Plasmid

#112005

Purpose

For enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV5 and AAV9

Insert

axon-GCaMP6s-P2A-mRuby3

Use

AAV

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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145-pGL3ctrl-3' UTR

Plasmid

#28175

Depositor

Michael Kastan

Article

Chen et al Genes Dev. 2010 Oct 1. 24(19):2146-56.

Insert

Luciferase flanked by tp53 UTRs (TP53 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Note that insert length is based on sum of the 2 …

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only
TFAM-SNAPf

Plasmid

#187909

Purpose

Mammalian cell expression of SNAPf-labeled TFAM

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

TFAM (TFAM Human)

Tags

SNAPf

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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nesprin TSmod 25

Plasmid

#125555

Purpose

Nesprin-2G force sensor with cerulean-F25-venus TSmodule

Depositor

Daniel Conway

Article

Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

Insert

mini-nesprin-2G (Syne2 Mouse)

Tags

cerulean, venus

Expression

Mammalian

Mutation

TSmod changed from mTFP to cerulean, flageliform …

Promoter

CMV

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-GPHN-FingR-mGold2s-CCR5TC

Plasmid

#231785

Purpose

Visualization of inhibitory post-synapse using GPHN-FingR-mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

GPHN-FingR-mGold2s-CCR5TC

Expression

Mammalian

Promoter

Actin promoter with CMV enhancer

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-R-iLACCO1

Plasmid

#208025

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-iLACCO1

Expression

Mammalian

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pOTTC407 - pAAV EF1a eGFP

Plasmid

#60058

Purpose

An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

enhanced Green Fluorescent Protein

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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EGFP-Ubiquitin

Plasmid

#187910

Purpose

Mammalian cell expression of EGFP-tagged Ubiquitin

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

UBB (UBB Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-R-iLACCO1.1

Plasmid

#208026

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-iLACCO1.1

Expression

Mammalian

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA4-NHK-MYC-His6

Plasmid

#185350

Purpose

Assessing of ubiquitination in cells

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

a1-antitrypsin Hong Kong Null mutant

Tags

Myc, 6xHis

Expression

Mammalian

Mutation

NHK mutant

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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