We narrowed to 29,617 results for: ple

1. pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NEL-mcherry-Tnos

   Plasmid

   #211842

   Purpose

   Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Inserts

   LRR-NEL
   GAL4-VP16-GR
   

   Use

   Synthetic Biology

   Tags

   HA, Myc, and mCherry

   Expression

   Plant

   Mutation

   Promoter

   6xUAS-35Smini and pUBQ10

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB2793

   Plasmid

   #64043

   Purpose

   pCfB2793 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Insert

   GFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 promoter and none

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Opto-alpha1AR

   Plasmid

   #106064

   Purpose

   Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-alpha1AR; Opto-alpha1AR; F6; 717)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-alpha1AR
   

   Use

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMK281 (mCherry2-Hygro)

   Plasmid

   #72797

   Purpose

   C-terminal tagging with mCherry2 using CRIPSR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mCherry2-Hygro
   

   Use

   CRISPR

   Tags

   mCherry2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. SAE1

   Plasmid

   #175241

   Purpose

   Bacterial expression and purification, combined with SAE2 to form the E1 enzyme heterodimer

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   SAE1 (SAE1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. SAE2-mGarnet

   Plasmid

   #175240

   Purpose

   Bacterial expression and purification, combined with SAE1 to form the E1 enzyme heterodimer

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   UBA2 (UBA2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3xFLAG-SENP6

   Plasmid

   #42887

   Depositor

   Edward Yeh

   Article

   Dou et al Mol Cell. 2010 Aug 13. 39(3):333-45.

   Insert

   SENP6 (SENP6 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Please see depositor comments

   Promoter

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-OSF-VPS15

   Plasmid

   #99326

   Purpose

   Component for mammalian expression of recombinant PI3KC3-C1 complex

   Depositor

   James Hurley

   Article

   Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

   Insert

   VPS15 (PIK3R4 Human)
   

   Use

   Tags

   Strep-Strep-Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pCfB2798

   Plasmid

   #63659

   Purpose

   pCfB2798 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Insert

   GFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330A_D10A-1x5

    Plasmid

    #58775

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK5-HA-GPR35

    Plasmid

    #204708

    Purpose

    expresses HA-GPR35 in mammalian cells

    Depositor

    William Kaelin

    Article

    Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

    Insert

    G protein-coupled receptor 35 (GPR35 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Grp1-PH pGEX2TK

    Plasmid

    #71379

    Purpose

    bacterial expression of PH domain for phosphoinositide binding assay

    Depositor

    Mark Lemmon

    Article

    Kavran et al J Biol Chem. 1998 Nov 13;273(46):30497-5

    Insert

    Grp1-PH (Cyth3 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa 264-391

    Promoter

    tac

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pCRISPomyces-1

    Plasmid

    #61736

    Purpose

    Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA

    Depositor

    Huimin Zhao

    Article

    Cobb et al ACS Synth Biol. 2014 Dec 8.

    Inserts

    sSpCas9
    tracrRNA
    crRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    BbsI-flanked lacZ cassette inserted in place of s…

    Promoter

    gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. Opto-D2R

    Plasmid

    #106068

    Purpose

    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-D2R; Opto-D2R; F10; 721)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-D2R
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJRH623

    Plasmid

    #171633

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-non-targeting
    mNeon
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a and U6

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAT007

    Plasmid

    #171634

    Purpose

    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-TRAC
    mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. p403-BrdU-Inc

    Plasmid

    #71789

    Purpose

    BrdU-incorporating plasmid with selectable marker HIS3

    Depositor

    Oscar Aparicio

    Article

    Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

    Inserts

    Thymidine Kinase
    ENT1 (SLC29A1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 and GPD

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Opto-M2R

    Plasmid

    #106070

    Purpose

    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-M2R; Opto-M2R; F12; 723)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-M2R
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMuLE_ENTR_CBF-GLuc_L3-L2

    Plasmid

    #113868

    Purpose

    Single pathway reporter; notch promoter-driven expression of secreted Gaussia luciferase; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    CBF (notch dependent promoter)
    GLuc
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTU424

    Plasmid

    #178983

    Purpose

    pACYC containing Candidatus “Scalindua brodae” TPR-CHAT (codon-optimized)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Insert

    TPR-CHAT
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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