We narrowed to 13,845 results for: Cas9

1. EC70364

   Plasmid

   #209515

   Purpose

   Cloning in complete guide expression cassettes, with Cas9

   Depositor

   Wendy Harwood, Cristobal Uauy

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. EC67844

   Plasmid

   #209514

   Purpose

   Cloning in complete guide expression cassettes, with Cas9

   Depositor

   Wendy Harwood, Cristobal Uauy

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. EC64434

   Plasmid

   #209518

   Purpose

   Cloning in complete guide expression cassettes, with Cas9 & Cas12a(D156R)

   Depositor

   Wendy Harwood, Cristobal Uauy

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMLS338

   Plasmid

   #73719

   Purpose

   SapTrap Single plasmid targeting vector for tagging Unc-32 with GFP by CRISPR/Cas9

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   Unc-32::GFP(Cbr-unc-119)
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPN10-gCTG

   Plasmid

   #114385

   Purpose

   pPN10 with gRNA for spCRISPR-Cas9 targeted to CTG repeat, PAM: CTG

   Depositor

   Vincent Dion

   Article

   Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

   Insert

   gCTG
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDB4279

   Plasmid

   #98698

   Purpose

   A control plasmid containing the intact bsdMX marker

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPN10-gCAG

   Plasmid

   #114384

   Purpose

   pPN10 with gRNA for spCRISPR-Cas9 targeted to CAG repeat, PAM: CAG

   Depositor

   Vincent Dion

   Article

   Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

   Insert

   gCAG
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRB1081

   Plasmid

   #71479

   Purpose

   Expresses gRNA for dpy-10 conversion using NGAG PAM and VQR Cas9 variant

   Depositor

   Andrew Fire

   Article

   Bell et al Genetics. 2015 Dec 17. pii: genetics.115

   Insert

   dpy-10 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRB1084

   Plasmid

   #71516

   Purpose

   Expresses gRNA for dpy-10 conversion using NGCG PAM and VRER Cas9 mutant

   Depositor

   Andrew Fire

   Article

   Bell et al Genetics. 2015 Dec 17. pii: genetics.115

   Insert

   dpy-10 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPN10-gDM1d

    Plasmid

    #114387

    Purpose

    pPN10 with gRNA for spCRISPR-Cas9 against the 3' UTR of the DMPK gene target: TGCGAACCAACGATAGGTG PAM: GGG

    Depositor

    Vincent Dion

    Article

    Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

    Insert

    gDM1d
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSC50

    Plasmid

    #104824

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Glyma.08g081600, Glyma.05g126600 (Hen1ab). Also expresses Cas9 from Gmubi promoter.

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Insert

    Glyma.08g081600, Glyma.05g126600
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX333

    Plasmid

    #64073

    Purpose

    Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.

    Depositor

    Andrea Ventura

    Article

    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    3XFLAG-Cas9

    Expression

    Mammalian

    Mutation

    Promoter

    CBh; U6

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiCRISPRv2 EGFP sgRNA1

    Plasmid

    #164932

    Purpose

    Expresses EGFP sgRNA1 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    sgRNA1 targeting Enhanced green fluorescent protein (verified for knockout)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCTRE-CD4

    Plasmid

    #114010

    Purpose

    Dox-inducible Cas9 expression with a CD4 selection marker

    Depositor

    Charles Rudin

    Article

    Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDIV515

    Plasmid

    #177703

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    tRNA-sgRNA-tRNA
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAS1yl

    Plasmid

    #73226

    Purpose

    Constitutive expression of Cas9 and sgRNA in Yarrowia lipolytica cells

    Depositor

    Sheng Yang

    Article

    Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR; In y. lipolytica

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    unknown

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiCRISPRv2 NT sgRNA3

    Plasmid

    #164929

    Purpose

    Expresses Non-targeting sgRNA3 control in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    Non-targeting sgRNA3 (verified for knockout)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Non-specific sgRNA

    Plasmid

    #109432

    Purpose

    MLM3636 backbone containing a gRNA that does not bind to any sequence in the human genome.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Non-specific gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHDR-USR-eGFP

    Plasmid

    #208830

    Purpose

    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed eGFP but without Cas9. The selective PuroR reporter gene was designed to be repaired only by the sgRNA/Cas9-triggered HDR.

    Depositor

    Zhiying Zhang

    Article

    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. PXL

    Plasmid

    #75349

    Purpose

    PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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