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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,824 results for: nls

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Showing: 3881 - 3900 of 18824 results

  5. pYUU

    Plasmid
    #159751
    Purpose
    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Publication
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Plant
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159751-plasmid-map-sequence-id-313821

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  6. TP63-T2A-Rox-EF1a-Rox-Venus-NLS repair template

    Plasmid
    #167971
    Purpose
    Tp63 reporter targeting repair template
    Depositor
    Emma Rawlins
    Publication
    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tp63 (TP63 Human)
    Use
    CRISPR; Donor template
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167971-plasmid-map-sequence-id-331132

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  11. AAV-pCamKII-Cre-pU6-SpCas9gRNAentry

    Plasmid
    #210391
    Purpose
    AAV plasmid encoding the CamKII promoter driving Cre expression, along with SpCas9 gRNA entry cassette (RFP dropout)
    Depositor
    Benjamin Kleinstiver
    Publication
    Huynh et al Nature. 2024 Nov;635(8037):168-177. doi:
    Insert
    AAV-(ITR)-pCamKII-NLS-Cre-WPRE-bGHpA-(rev)-SpCas9_gRNA-BsmBI_RFPcassette-hU6-(ITR) (NJA158)
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Promoter
    CamKII promoter for Cre, human U6 promoter for Sp…
    Available since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    210391-plasmid-map-sequence-id-415458

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  14. pCMV-bpNLS-Stab-exin21-eeBxb1-Stab (CJT607)

    Plasmid
    #248212
    Purpose
    bpNLS-, stabilon-, and exin21-tagged eeBxb1 recombinase, expressed from a CMV promoter.
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586
    Insert
    bpNLS-Stab-exin21-eeBxb1-Stab
    Use
    CRISPR; In vitro transcription
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    eeBxb1(V74A, E229K, V375I)
    Promoter
    CMV and T7
    Available since
    May 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    248212-plasmid-map-sequence-id-509831

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  18. TV_LGR5-ires-mNeon-nls-p2a-dtr-wpre_pgk-puro

    Plasmid
    #251173
    Purpose
    Targeting vector for C-terminal knockin at LGR5 locus to enable reporting of expression by mNeonGreen and depletion of positive cells with DTR
    Depositor
    Hugo Snippert
    Insert
    ires-mNeon-nls-p2a-dtr-wpre_pgk-puro flanked by homology arms for C-terminal targeting of human LGR5 (LGR5 Human)
    Use
    CRISPR
    Tags
    ires-mNeon-nls-p2a-dtr
    Available since
    Feb. 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    251173-plasmid-map-sequence-id-500690

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Showing: 3881 - 3900 of 18824 results