We narrowed to 9,154 results for: nls
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pDB22
Plasmid#61342PurposeMammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)DepositorInsertmCherry
TagsGS linker-AsLov2-cMyc1A NLS and PKIt NESExpressionMammalianPromoterCMVAvailable SinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
StrKDEL-IRES_mScarleti_Sec23A
Plasmid#117273PurposeExpresses an Str-KDEL Hook for the RUSH system and separately mScarleti-Sec23ADepositorInsertsTagsmScarlet-iExpressionMammalianMutationHuman Sec23A; Sequencing showed a mutation in Sec…PromoterCMV and IRESAvailable SinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pB-dCas9-PBE
Plasmid#98159PurposeExpresses CRISPR base editor in maizeDepositorInsertNLS-APOBEC1-XTEN-dCas9-UGI-NLS
UseMaizeExpressionPlantPromoterUbiAvailable SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
p4459 FLAG-hPLIC-1 NTF
Plasmid#8664DepositorInserthPLIC-1 (UBQLN1 Human)
TagsFLAGExpressionMammalianMutationN-terminal fragment: amino acids 1-112 of hPLIC-1…Available SinceJune 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
MSP2283
Plasmid#70702PurposeBacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialPromoterT7Available SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-PBE
Plasmid#98161PurposeExpresses CRISPR base editor in protoplastsDepositorInsertNLS-APOBEC1-XTEN-dCas9-UGI-NLS
ExpressionPlantPromoterUbiAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
A3A-PBE-ΔUGI
Plasmid#119770PurposeConversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)DepositorInsertAPOBEC3A-nCas9-ΔUGI-NLS
TagsNLSExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMM2-rPMCA2a
Plasmid#47762Purposemammalian expression of rat PMCA2aDepositorAvailable SinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET42b-A3A-PBE-ΔUGI
Plasmid#119771PurposeTo express the recombinant APOBEC3A in vitroDepositorInsertAPOBEC3A-nCas9-NLS
Tags6*HIS and NLSExpressionBacterialMutationnCas9 has a mutation D10A in wild type SpCas9PromoterT7 promoterAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSP2266
Plasmid#70705PurposeBacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialPromoterT7Available SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-CB1R-V2tail-TevN-BLITz1-TetR-VP16
Plasmid#89872Purposeexpresses CB1R-iTango component in mammalian cellsDepositorInsertCB1R-V2tail-TevN-BLITz1-TetR-VP16
ExpressionMammalianPromoterCMVAvailable SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD27-52_K5S
Plasmid#98679PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5SDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
Tags6xHisExpressionBacterialMutationK1838S, K1859S, K1866S, K1873S, K1887SAvailable SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD38-52
Plasmid#98685PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase IIDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
Tags6xHisExpressionBacterialMutationheptads 38-52, residues 1853-1970Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
kank2_pJet1.2
Plasmid#121980Purposefor generating kank2 riboprobes for WISH in zebrafishDepositorAvailable SinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-mCE(K294A)
Plasmid#82461PurposeExpression of myc epitope and catalytic inactive form of murine mRNA Capping Enzyme (K294A) in mammalian cellsDepositorInsertmCE (K294A) (Rngtt Mouse)
TagsmycExpressionMammalianMutationLysine 294 changed to AlaninePromoterCMVAvailable SinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMGF173
Plasmid#96999PurposeNLS-GFP-lacZ leu1 insertion vectorDepositorInsertlacZ
TagsNLS-GFPExpressionYeastPromoterPnmt1Available SinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
