We narrowed to 2,346 results for: puc19

1. pHS1871

   Plasmid

   #205976

   Purpose

   U6-JAGBLH010000220 Cas8-HNH DR-BpiI-DR

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas8-HNH DR-BpiI-DR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. LADL Anchor

   Plasmid

   #127664

   Purpose

   Encodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoter

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Insert

   dCas9-CIBN-2A-Puro
   

   Use

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 (D10A,H840A) mutant

   Promoter

   EF1a

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJB556

   Plasmid

   #115966

   Purpose

   SGP insert for replicon MoClo

   Depositor

   Ron Weiss

   Article

   Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

   Insert

   Lvl0 -98/15 SGP 2xTetApt-STOP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pREMI-ZA

   Plasmid

   #183736

   Purpose

   Plasmid for a restriction enzyme mediated integration (REMI) in Komagataella phaffii

   Depositor

   Jun Ishii

   Article

   Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

   Insert

   Sh ble
   

   Use

   Random insertional gene deletion in komagataella …

   Tags

   Expression

   Mutation

   Promoter

   TEF1 promoter

   Available since

   March 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. ATP1A1_T804N_hPGK1_EBFP_Donor

   Plasmid

   #187455

   Purpose

   HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the EBFP cassette to ATP1A1 intron 17.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1-T804N EBFP donor (ATP1A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK1

   Available since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPN062

   Plasmid

   #91595

   Purpose

   Express sgRNA targeting human DFNA5

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   DFNA5 (GSDME Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPN063

   Plasmid

   #91596

   Purpose

   Express sgRNA targeting human DFNA5

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   DFNA5 (GSDME Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. phFluc-WT

   Plasmid

   #177714

   Purpose

   Cytosolic firefly luciferase reporter previously described in Shcherbakov et al. 2019

   Depositor

   Erik Böttger

   Article

   Shcherbakov et al Commun Biol. 2019 Oct 17;2:381. doi: 10.

   Insert

   hFluc WT
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-C-mNeon-Blast-TUBB4B

   Plasmid

   #207786

   Purpose

   Donor template for mNeon-2A-Blast insertion into the C-terminus of the TUBB4B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBB4B Addgene #207785

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBB4B Homology Arms flanking a mNeon-Blast Cassette (TUBB4B Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-C-moxGFP-Puro-H2BC11-MMEJ

    Plasmid

    #207760

    Purpose

    MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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