We narrowed to 15,931 results for: nans

1. pLysscys255

   Plasmid

   #36429

   Depositor

   Pete Lollar, Emine Gunhan

   Article

   Gunhan et al Protein Expr Purif. 2008 Apr;58(2):203-9

   Insert

   Saporin-3/cys255
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   cys added at position 255

   Promoter

   T7

   Available since

   May 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJH2970

   Plasmid

   #100954

   Purpose

   Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HIS3MX marker

   Depositor

   James Haber

   Article

   Ranjith et al Protocol Exchange (2017)

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28Teb1

   Plasmid

   #28173

   Depositor

   Kathleen Collins

   Article

   Min et al Mol Cell. 2009 Nov 25. 36(4):609-19.

   Insert

   Teb1
   

   Use

   Tags

   6xhistidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pmVirD2-GFP

   Plasmid

   #90315

   Purpose

   mVirD2-GFP expression plasmid

   Depositor

   Bjorn Hogberg

   Article

   Bernardinelli et al Nucleic Acids Res. 2017 Oct 13;45(18):e1

   Insert

   A. tumefaciens VirD2 from aa 1 to 204 - GFP
   

   Use

   Tags

   6xHis and GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7/T5 IPTG inducible

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNIC-HIS6-GST-TEV-GG

   Plasmid

   #215801

   Purpose

   Cloning of target gene with N terminal His6 GST tag and TEV cleavage

   Depositor

   ASAP Discovery, Frank von Delft

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pRSETB PKA Cat

   Plasmid

   #14920

   Depositor

   Susan Taylor

   Article

   Taylor et al Biochim Biophys Acta. 2005 Dec 30. 1754(

   Insert

   PKA catalytic subunit alpha (Prkaca Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2007

   Availability

   Academic Institutions and Nonprofits only

7. CIT96u

   Plasmid

   #128018

   Purpose

   K77A

   Depositor

   Uwe Sauer

   Article

   Ewald et al PLoS One. 2011;6(12):e28245. doi: 10.137

   Insert

   CitA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKM342

   Plasmid

   #71486

   Purpose

   Contains loxP-hyg-loxP cassette for recombineering; HygR, AmpR.

   Depositor

   Kenan Murphy

   Article

   Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

   Insert

   loxP-hyg-loxP cassette
   

   Use

   Source of loxp-hyg-loxp for pcr amplification.

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTK164

   Plasmid

   #13533

   Depositor

   Wolf Frommer

   Article

   Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

   Insert

   FLIPW-CTY
   

   Use

   Tags

   ECFP and Venus

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 27, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNIC-HIS6-THRX-TEV-GG

    Plasmid

    #215796

    Purpose

    Cloning of target gene with N terminal His6 Thioredoxin tag and TEV cleavage

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pMAK462

    Plasmid

    #140700

    Purpose

    NBαMs/Rt-IgG1-Fc

    Depositor

    Takayuki Iwaki

    Article

    Iwaki et al Protein Expr Purif. 2020 Feb 13;170:1056

    Insert

    NBαMs/Rt-IgG1-Fc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNIC-NHSTIIT-AVITAG-GG

    Plasmid

    #215802

    Purpose

    Cloning of target gene with N terminal His6 Twin Strep tag and TEV cleavage non cleavable N-terminal AVITAG

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pET24a MDHC1_ARATH

    Plasmid

    #204237

    Purpose

    Bacterial expression of Arabidopsis cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDHC1_ARATH (AT1G04410 Mustard Weed)
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FLIParaF.Ec-250n

    Plasmid

    #18855

    Depositor

    Wolf Frommer

    Article

    Kaper et al Biotechnol Biofuels. 2008 Jun 3. 1(1):11

    Insert

    arabinose FRET sensor
    

    Use

    Tags

    CFP and Venus

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pRSET FLIPmal-2u

    Plasmid

    #14479

    Depositor

    Wolf Frommer

    Article

    Fehr et al Proc Natl Acad Sci U S A. 2002 Jul 23. 9

    Insert

    FLIPmal
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    5AA

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDH22

    Plasmid

    #83798

    Purpose

    Useful for tagging yeast proteins with N-terminal YFP tag; G418 selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    YFP
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMAK463

    Plasmid

    #140701

    Purpose

    NBαMs-IgK

    Depositor

    Takayuki Iwaki

    Article

    Iwaki et al Protein Expr Purif. 2020 Feb 13;170:1056

    Insert

    NBαMs-IgK
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCasY1

    Plasmid

    #87687

    Purpose

    p15A plasmid expressing CasY1

    Depositor

    Jennifer Doudna

    Article

    Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

    Insert

    CasY1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBS4

    Plasmid

    #83793

    Purpose

    Useful for tagging yeast proteins with C-terminal CFP tag; hygromycin-B selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    CFP
    

    Use

    Tags

    CFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAR-IF reporter

    Plasmid

    #16628

    Depositor

    Bert Vogelstein

    Article

    Nicolaides et al Mol Cell Biol. 1998 Mar . 18(3):1635-41.

    Insert

    CAR-IF
    

    Use

    Tags

    beta-galactosidase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only