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We narrowed to 17,501 results for: puro

Showing: 3901 - 3920 of 17501 results

  2. 4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO

    Plasmid
    #68356
    Purpose
    -The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoter
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
    FlpO recombinase
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Mutation
    Promoter
    Synthetic Probasin ARRx2 and U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68356-plasmid-map-sequence-id-119628

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  3. pDG459

    Plasmid
    #100901
    Purpose
    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100901-plasmid-map-sequence-id-197197

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  4. CROP-seq-opti

    Plasmid
    #106280
    Purpose
    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.
    Depositor
    Jay Shendure
    Article
    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    106280-plasmid-map-sequence-id-354474

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  12. pLKO.1 mCherry

    Plasmid
    #128073
    Purpose
    puro exchanged to mCherry
    Depositor
    Oskar Laur
    Insert
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    no
    Promoter
    Available since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128073-plasmid-map-sequence-id-255273

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  13. CROPSeq-BFP

    Plasmid
    #196718
    Purpose
    CROP-Seq library plasmid with Chen tracrRNA (PMID: 24360272) and TagBFP
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196718-plasmid-map-sequence-id-388068

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  14. pAK_DR30_CasRx_mCherry

    Plasmid
    #134844
    Purpose
    All in one overexpression of CasRx and guideRNA
    Depositor
    Michael Yaffe
    Insert
    RfxCas13d
    Use
    CRISPR
    Tags
    T2A mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Nov. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    134844-plasmid-map-sequence-id-266704

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  15. pAK_DR30_CasRx_GFP

    Plasmid
    #134843
    Purpose
    All in one overexpression of CasRx and guideRNA
    Depositor
    Michael Yaffe
    Insert
    RfxCas13d
    Use
    CRISPR
    Tags
    T2A EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    134843-plasmid-map-sequence-id-266705

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  16. PL-SIN-PGK-EiP

    Plasmid
    #21312
    Purpose
    Control Lentiviral vector with ubiquitous PGK promoter, expresses EGFP and Puro resistance
    Depositor
    James Ellis
    Article
    Hotta et al Nat Methods. 2009 May . 6(5):370-6.
    Insert
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available since
    June 1, 2009
    Availability
    Academic Institutions and Nonprofits only
    21312-plasmid-map-sequence-id-9853

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  17. pDG462

    Plasmid
    #100903
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100903-plasmid-map-sequence-id-196607

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Showing: 3901 - 3920 of 17501 results