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pcDNA3.1(+)-mCherry-p115

Plasmid

#181996

Purpose

Mammalian expression vector encoding p115 RhoGEF tagged with mCherry.

Depositor

Jin Zhang

Article

Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

Insert

mCherry-p115 (ARHGEF1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pRGEN-CMV-VPR-L1-CjCas9 V-D

Plasmid

#169913

Purpose

Expression of a VPR-dCjCas9 fusion protein to activate gene expression.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Insert

dCjCas9

Use

CRISPR

Tags

2xSV40NLS, SV40NLS-HA, and VPR (VP64-p65-Rta)

Expression

Mammalian

Mutation

D8A, H559A in cjCas9

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5A 378-1027

Plasmid

#134611

Purpose

Mammalian expression of mouse KIF5A 378-1027

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF5A (Kif5a Mouse)

Tags

Halo

Expression

Mammalian

Mutation

WT with deletion of the 377 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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SFRS16

Plasmid

#156009

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SFRS16 (CLASRP Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pg-proPHD2-PHD2-EGFP

Plasmid

#87210

Purpose

PHD2-EGFP expressed under the control of the PHD2 promoter

Depositor

Violaine Sée

Article

Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

Insert

PHD2 (EGLN1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

H313N

Promoter

PHD2

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro1 Δ593-618

Plasmid

#47895

Purpose

Expresses myc tagged Miro1 lacking transmembrane domain

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro1 Δ593-618 (RHOT1 Human)

Tags

myc

Expression

Mammalian

Mutation

Δ593-618, lacks transmembrane domain

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pLJM1-PHD2

Plasmid

#223552

Purpose

Lentivirus transfer plasmid for expression of full length human PHD2

Depositor

Somshuvra Mukhopadhyay

Article

Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

Insert

PHD2 (EGLN1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCold I-Hero11

Plasmid

#187928

Purpose

Bacterial expression of heat-resistant obscure (Hero) protein Hero11

Depositor

Yukihide Tomari

Article

Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

Insert

Hero11 (C19orf53 Human)

Tags

Flag-6xHis

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pET19-MTdTc302

Plasmid

#207458

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

MTdTc302 (Dntt Mouse)

Tags

10xHis-MBP

Expression

Bacterial

Mutation

Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pPW3760 : CMVd3-HsIRE1-K599A_KinaseDead-HaloTag

Plasmid

#185682

Purpose

Low-level transient expression of kinase-dead K599A HsIRE1 with a C-terminal HaloTag.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 (ERN1 Synthetic, Human)

Tags

HaloTag

Expression

Mammalian

Mutation

K599A

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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Lrrc4c-Fc-His

Plasmid

#72085

Purpose

Expresses the extracellular region of the LRRC4C protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Lrrc4c (Lrrc4c Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK2 K235R

Plasmid

#80872

Purpose

mammalian expression of ALK2 K235R

Depositor

Aristidis Moustakas

Article

Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

Insert

ALK2 (ACVR1 Human)

Tags

HA

Expression

Mammalian

Mutation

K235R

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-CMV-Grid2(I677C)

Plasmid

#164782

Purpose

cysteine mutated glutamate delta2 receptor (I677C) for the attachment of a photoswitchable tethered ligand

Depositor

Alexandre Mourot

Article

Lemoine et al Elife. 2020 Oct 28;9. pii: 59026. doi: 1

Insert

Grid2 I677C (Grid2 Mouse)

Expression

Mammalian

Mutation

I677C

Promoter

CMV

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-KLF12/pcDNA3.1-Neo

Plasmid

#81069

Purpose

Expression of epitope-tagged human KLF12 in mammalian cells.

Depositor

Julian Downward

Article

Godin-Heymann et al Oncogene. 2016 Jun 23;35(25):3324-34. do

Insert

FLAG-KLF12 (KLF12 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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gh55

Plasmid

#106728

Purpose

expression of gRNA targeting B4GALT7

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B4GALT7 (B4GALT7 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC45A4

Plasmid

#132179

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC45A4 (SLC45A4 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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FXYD2-luc

Plasmid

#31056

Depositor

Gerhart Ryffel

Article

Adalat et al J Am Soc Nephrol. 2009 May . 20(5):1123-

Insert

FXYD2 (FXYD2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

FXYD2 promoter from -2382 to -1

Available Since

Aug. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pGEX Lyn-SH2

Plasmid

#46452

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Lyn (LYN Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 106-213

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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Flrt1-Fc-His

Plasmid

#72073

Purpose

Expresses the extracellular region of the FLRT1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Flrt1 (Flrt1 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pHH0103_LYN-1/1

Plasmid

#91536

Purpose

Protein expression and purification of human SH3 domain construct LYN-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

LYN-1/1 (LYN Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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