We narrowed to 6,241 results for: nls

1. pLV-REPORT(PGK/CMV)-GRE

   Plasmid

   #172336

   Purpose

   Gal4 Response element

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   monomeric nuclear Tomato
   d2eGFP
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3x SV40 NLS

   Expression

   Mammalian

   Promoter

   PGK/CMV

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPIK16045_pX330_Flag-SuperFi-Cas9

   Plasmid

   #184370

   Purpose

   Mammalian expression of SuperFi-Cas9 nuclease

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   3X FLAG and NLS

   Expression

   Mammalian

   Mutation

   Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, K…

   Promoter

   CBh

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQAT07

   Plasmid

   #223379

   Purpose

   T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   AtUBQ10-SpRY-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3xFLAG-dCas9/pMXs-puro

   Plasmid

   #51240

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available Since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSQT834

   Plasmid

   #53371

   Purpose

   Csy4 and Cas9 nuclease expression plasmid

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   Csy4-T2A-Cas9-NLS
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pREDIT_MCP-RecT

   Plasmid

   #164803

   Purpose

   mammalian-optimized expression of SSAP RecT fused to MCP (for MS2 aptamer), pLenti-EF1A-MCP-EXTEN-RecT-NLS

   Depositor

   Le Cong

   Article

   Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

   Insert

   RecT
   

   Expression

   Mammalian

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ167

   Plasmid

   #69309

   Purpose

   Gateway entry vector with Cas9p

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   Cas9p (Plant codon-optimized; high GC content at 5 prime region)
   

   Use

   CRISPR

   Mutation

   NLS fusion

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-PE2-VQR

   Plasmid

   #159982

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-VQR
   

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. 3xFLAG-dCas9/pMSCVhyg

   Plasmid

   #134323

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ubiq-Cas9.874W

    Plasmid

    #112686

    Purpose

    Express hSpCas9 under Ubiquitin-63E promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    hSpCas9
    

    Tags

    3x FLAG, EGFP, and NLS

    Expression

    Insect

    Mutation

    Humanized Cas9 bacterial sequence

    Promoter

    Ubiquitin-63E promoter

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMLM3705

    Plasmid

    #47754

    Purpose

    Expresses mammalian cell codon-optimized dCas9-VP64

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    codon optimized dCas9-VP64
    

    Use

    CRISPR

    Tags

    3x FLAG, NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A and H840A mutations in Cas9 (catalytically i…

    Promoter

    CMV

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-I-SceI-Trex2

    Plasmid

    #44024

    Depositor

    Jeremy Stark

    Article

    Gunn et al J Biol Chem. 2011 Dec 9;286(49):42470-82

    Insert

    I-SceI fused to mTREX2
    

    Tags

    NLS-HA

    Expression

    Mammalian

    Promoter

    pCAGGS

    Available Since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMpGWB115

    Plasmid

    #68569

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    Nuclear localization signal-tagged Citrine (Citri…

    Expression

    Plant

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-Cas9-162A

    Plasmid

    #84918

    Purpose

    for in vitro transcription of Cas9 with polyA tail encoded

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Cas9
    

    Tags

    Flag, NLS, and polyA tail

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. sABE v3.22_N

    Plasmid

    #209553

    Purpose

    pCMV-bpSV40 NLS-TadA8e(2-76)-2xFRB-BGHpA

    Depositor

    Xue Gao

    Article

    Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

    Insert

    TadA-8e(2-76)-2xFRB
    

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pH-PABE-7-esgRNA

    Plasmid

    #115620

    Purpose

    Targeted A to G in rice

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    wtTadA-TadA7.10-nCas9-3*NLS
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL16

    Plasmid

    #158742

    Purpose

    Expresses influenza A virus tetrameric neuraminidase fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

    Insert

    Neuraminidase A/NL/354/16 H3N2
    

    Tags

    TwinStrep_sfGFP_tetrabrachion

    Expression

    Mammalian

    Mutation

    AA61-469

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMB34

    Plasmid

    #122245

    Purpose

    Lentivirus delivery for stable expression of LbCas12a-VPR

    Depositor

    Darjus Tschaharganeh

    Article

    Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

    Insert

    mammalian codon-optimized Lachnospiraceae bacterium Cas12a fused to VPR activator
    

    Use

    Lentiviral

    Tags

    NLS and VPR activator

    Promoter

    EFs

    Available Since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBK-YE1-BE3

    Plasmid

    #85174

    Purpose

    Expresses YE1-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    YE1-BE3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. EC2_3_dCas9_Mxi1_sgRNA

    Plasmid

    #163708

    Purpose

    Yeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressor

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    Mxi1+SV40 NLS
    

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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